PDB entry 6eec

View 6eec on RCSB PDB site
Description: mycobacterium tuberculosis rnap promoter unwinding intermediate complex with rbpa/card and ap3 promoter captured by corallopyronin
Deposited on 2018-08-13, released 2018-11-21
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 3.55 Å
R-factor: N/A
AEROSPACI score: 0.09 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoA
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoA
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: DNA-directed RNA polymerase subunit beta
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoB, SAMEA2682864_01701
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: DNA-directed RNA polymerase subunit beta'
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoC
    Database cross-references and differences (RAF-indexed):
    • Uniprot A5U053 (2-End)
      • expression tag (0-1)
  • Chain 'E':
    Compound: DNA-directed RNA polymerase subunit omega
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoZ, ERS007672_03979, ERS007703_04032, ERS007720_04749, ERS027652_00548, ERS027654_02543, ERS027656_03959, ERS124361_02246
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: RNA polymerase sigma factor SigA
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: sigA, mysA, rpoD, rpoV
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: RNA polymerase-binding protein RbpA
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rbpA
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: RNA polymerase-binding transcription factor CarD
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: carD
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: DNA (65-mer)
    Species: MYCOBACTERIUM TUBERCULOSIS, synthetic [TaxId:1773]
  • Chain 'P':
    Compound: DNA (63-mer)
    Species: MYCOBACTERIUM TUBERCULOSIS, synthetic [TaxId:1773]
  • Heterogens: C0L, ZN, MG

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6eecA (A:)
mlisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvl
hefttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhn
pgmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvda
trveqrtdfdklildvetknsisprdalasagktlvelfglarelnveaegieigpspae
adhiasfalpiddldltvrsynclkregvhtvgelvartesdlldirnfgqksidevkik
lhqlglslkdsppsfdpsevagydvatgtwstegaydeqdyaeteql

    Sequence, based on observed residues (ATOM records):
    >6eecA (A:)
lisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvlh
efttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhnp
gmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvdat
rveqrtdfdklildvetknsisprdalasagktlvelfglareln

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6eecB (B:)
mlisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvl
hefttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhn
pgmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvda
trveqrtdfdklildvetknsisprdalasagktlvelfglarelnveaegieigpspae
adhiasfalpiddldltvrsynclkregvhtvgelvartesdlldirnfgqksidevkik
lhqlglslkdsppsfdpsevagydvatgtwstegaydeqdyaeteql

    Sequence, based on observed residues (ATOM records):
    >6eecB (B:)
mlisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvl
hefttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhn
pgmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvda
trveqrtdfdklildvetknsisprdalasagktlvelfglarelnveaegieigps

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6eecC (C:)
madsrqsktaaspspsrpqsssnnsvpgapnrvsfaklreplevpglldvqtdsfewlig
sprwresaaergdvnpvggleevlyelspiedfsgsmslsfsdprfddvkapvdeckdkd
mtyaaplfvtaefinnntgeiksqtvfmgdfpmmtekgtfiingtervvvsqlvrspgvy
fdetidkstdktlhsvkvipsrgawlefdvdkrdtvgvridrkrrqpvtvllkalgwtse
qiverfgfseimrstlekdntvgtdealldiyrklrpgepptkesaqtllenlffkekry
dlarvgrykvnkklglhvgepitsstlteedvvatieylvrlhegqttmtvpggvevpve
tddidhfgnrrlrtvgeliqnqirvgmsrmervvrermttqdveaitpqtlinirpvvaa
ikeffgtsqlsqfmdqnnplsglthkrrlsalgpgglsreraglevrdvhpshygrmcpi
etpegpnigligslsvyarvnpfgfietpyrkvvdgvvsdeivyltadeedrhvvaqans
pidadgrfveprvlvrrkageveyvpssevdymdvsprqmvsvatamipflehddanral
mganmqrqavplvrseaplvgtgmelraaidagdvvvaeesgvieevsadyitvmhdngt
rrtyrmrkfarsnhgtcanqcpivdagdrveagqviadgpctddgemalgknllvaimpw
eghnyedaiilsnrlveedvltsihieeheidardtklgaeeitrdipnisdevladlde
rgivrigaevrdgdilvgkvtpkgeteltpeerllraifgekarevrdtslkvphgesgk
vigirvfsrededelpagvnelvrvyvaqkrkisdgdklagrhgnkgvigkilpvedmpf
ladgtpvdiilnthgvprrmnigqilethlgwcahsgwkvdaakgvpdwaarlpdellea
qpnaivstpvfdgaqeaelqgllsctlpnrdgdvlvdadgkamlfdgrsgepfpypvtvg
ymyimklhhlvddkiharstgpysmitqqplggkaqfggqrfgemecwamqaygaaytlq
elltiksddtvgrvkvyeaivkgenipepgipesfkvllkelqslclnvevlssdgaaie
lregededleraaanlginlsrnesasvedlalarhggs

    Sequence, based on observed residues (ATOM records):
    >6eecC (C:)
nsvpgapnrvsfaklreplevpglldvqtdsfewligsprwresaaergdvnpvggleev
lyelspiedfsgsmslsfsdprfddvkapvdeckdkdmtyaaplfvtaefinnntgeiks
qtvfmgdfpmmtekgtfiingtervvvsqlvrspgvyfdetidkstdktlhsvkvipsrg
awlefdvdkrdtvgvridrkrrqpvtvllkalgwtseqiverfgfseimrstlekdntvg
tdealldiyrklrpgepptkesaqtllenlffkekrydlarvgrykvnkklglhvgepit
sstlteedvvatieylvrlhegqttmtvpggvevpvetddidhfgnrrlrtvgeliqnqi
rvgmsrmervvrermttqdveaitpqtlinirpvvaaikeffgtsqlsqfmdqnnplsgl
thkrrlsalgpgglsreraglevrdvhpshygrmcpietpegpnigligslsvyarvnpf
gfietpyrkvvdgvvsdeivyltadeedrhvvaqanspidadgrfveprvlvrrkageve
yvpssevdymdvsprqmvsvatamipflehddanralmganmqrqavplvrseaplvgtg
melraaidagdvvvaeesgvieevsadyitvmhdngtrrtyrmrkfarsnhgtcanqcpi
vdagdrveagqviadgpctddgemalgknllvaimpweghnyedaiilsnrlveedvlts
ihieeheidardtklgaeeitrdipnisdevladldergivrigaevrdgdilvgkvtpk
geteltpeerllraifgekarevrdtslkvphgesgkvigirvfsrededelpagvnelv
rvyvaqkrkisdgdklagrhgnkgvigkilpvedmpfladgtpvdiilnthgvprrmnig
qilethlgwcahsgwkvdaakgvpdwaarlpdelleaqpnaivstpvfdgaqeaelqgll
sctlpnrdgdvlvdadgkamlfdgrsgepfpypvtvgymyimklhhlvddkiharstgpy
smitqqplggkaqfggqrfgemecwamqaygaaytlqelltiksddtvgrvkvyeaivkg
enipepgipesfkvllkelqslclnvevlss

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6eecD (D:)
gamldvnffdelriglataedirqwsygevkkpetinyrtlkpekdglfcekifgptrdw
ecycgkykrvrfkgiicercgvevtrakvrrermghielaapvthiwyfkgvpsrlgyll
dlapkdlekiiyfaayvitsvdeemrhnelstleaemaverkavedqrdgelearaqkle
adlaeleaegakadarrkvrdggeremrqirdraqreldrlediwstftklapkqlivde
nlyrelvdrygeyftgamgaesiqklienfdidaeaeslrdvirngkgqkklralkrlkv
vaafqqsgnspmgmvldavpvippelrpmvqldggrfatsdlndlyrrvinrnnrlkrli
dlgapeiivnnekrmlqesvdalfdngrrgrpvtgpgnrplkslsdllkgkqgrfrqnll
gkrvdysgrsvivvgpqlklhqcglpklmalelfkpfvmkrlvdlnhaqniksakrmver
qrpqvwdvleeviaehpvllnraptlhrlgiqafepmlvegkaiqlhplvceafnadfdg
dqmavhlplsaeaqaearilmlssnnilspasgrplamprldmvtglyylttevpgdtge
yqpasgdhpetgvysspaeaimaadrgvlsvrakikvrltqlrppveieaelfghsgwqp
gdawmaettlgrvmfnellplgypfvnkqmhkkvqaaiindlaerypmivvaqtvdklkd
agfywatrsgvtvsmadvlvpprkkeildhyeeradkvekqfqrgalnhdernealveiw
keatdevgqalrehypddnpiitivdsgatgnftqtrtlagmkglvtnpkgefiprpvks
sfregltvleyfinthgarkgladtalrtadsgyltrrlvdvsqdvivrehdcqtergiv
velaerapdgtlirdpyietsayartlgtdavdeagnvivergqdlgdpeidallaagit
qvkvrsvltcatstgvcatcygrsmatgklvdigeavgivaaqsigepgtqltmrtfhqg
gvgeditgglprvqelfearvprgkapiadvtgrvrledgerfykitivpddggeevvyd
kiskrqrlrvfkhedgservlsdgdhvevgqqlmegsadphevlrvqgprevqihlvrev
qevyraqgvsihdkhievivrqmlrrvtiidsgsteflpgslidraefeaenrrvvaegg
epaagrpvlmgitkaslatdswlsaasfqettrvltdaaincrsdklnglkenviigkli
pagtginryrniavqpteearaaaytipsyedqyyspdfgaatgaavplddygysdyrhh
hhhhhh

    Sequence, based on observed residues (ATOM records):
    >6eecD (D:)
gamldvnffdelriglataedirqwsygevkkpetinyrtlkpekdglfcekifgptrdw
ecycgkykrvrfkgiicercgvevtrakvrrermghielaapvthiwyfkgvpsrlgyll
dlapkdlekiiyfaayvitsvdeemrhnelstleaemaverkavedqrdgelearaqkle
adlaeleaegakadarrkvrdggeremrqirdraqreldrlediwstftklapkqlivde
nlyrelvdrygeyftgamgaesiqklienfdidaeaeslrdvirngkgqkklralkrlkv
vaafqqsgnspmgmvldavpvippelrpmvqldggrfatsdlndlyrrvinrnnrlkrli
dlgapeiivnnekrmlqesvdalfdngrrgrpvtgpgnrplkslsdllkgkqgrfrqnll
gkrvdysgrsvivvgpqlklhqcglpklmalelfkpfvmkrlvdlnhaqniksakrmver
qrpqvwdvleeviaehpvllnraptlhrlgiqafepmlvegkaiqlhplvceafnadfdg
dqmavhlplsaeaqaearilmlssnnilspasgrplamprldmvtglyylttevpgdtge
yqpasgdhpetgvysspaeaimaadrgvlsvrakikvrltqlrppveieaelfghsgwqp
gdawmaettlgrvmfnellplgypfvnkqmhkkvqaaiindlaerypmivvaqtvdklkd
agfywatrsgvtvsmadvlvpprkkeildhyeeradkvekqfqrgalnhdernealveiw
keatdevgqalrehypddnpiitivdsgatgnftqtrtlagmkglvtnpkgefiprpvks
sfregltvleyfinthgarkgladtalrtadsgyltrrlvdvsqdvivrehdcqtergiv
velaerapdgtlirdpyietsayartlgtdavdeagnvivergqdlgdpeidallaagit
qvkvrsvltcatstgvcatcygrsmatgklvdigeavgivaaqsigepgtqltmtgglpr
vqelfearvprgkapiadvtgrvrledgerfykitivpddggeevvydkiskrqrlrvfk
rvlsdgdhvevgqqlmegsadphevlrvqgprevqihlvrevqevyraqgvsihdkhiev
ivrqmlrrvtiidsgsteflpgslidraefeaenrrvvaeggepaagrpvlmgitkasla
tdswlsaasfqettrvltdaaincrsdklnglkenviigklipagtginryrniavqpte
earaaa

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6eecE (E:)
gsisqsdaslaavpavdqfdpssgasggydtplgitnppidelldrvsskyalviyaakr
arqindyynqlgegileyvgplvepglqekplsialreihadllehtege

    Sequence, based on observed residues (ATOM records):
    >6eecE (E:)
ggydtplgitnppidelldrvsskyalviyaakrarqindyynqlgegileyvgplvepg
lqekplsialreihadllehteg

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6eecF (F:)
gphmaatkastatdepvkrtatkspaasasgaktgakrtaaksasgsppakratkpaars
vkpasapqdtttstipkrktraaaksaaakapsarghatkprapkdaqheaatdpedald
sveeldaepdldvepgedldldaadlnlddleddvapdadddldsgddedhedleaeaav
apgqtadddeeiaeptekdkasgdfvwdedesealrqarkdaeltasadsvraylkqigk
vallnaeeevelakrieaglyatqlmtelsergeklpaaqrrdmmwicrdgdraknhlle
anlrlvvslakrytgrgmafldliqegnlgliravekfdytkgykfstyatwwirqaitr
amadqartiripvhmvevinklgriqrellqdlgreptpeelakemditpekvleiqqya
repisldqtigdegdsqlgdfiedseavvavdavsftllqdqlqsvldtlsereagvvrl
rfgltdgqprtldeigqvygvtrerirqiesktmsklrhpsrsqvlrdyld

    Sequence, based on observed residues (ATOM records):
    >6eecF (F:)
sealrqarkdaeltasadsvraylkqigkvallnaeeevelakrieaglyatqlmtelse
rgeklpaaqrrdmmwicrdgdraknhlleanlrlvvslakrytgrgmafldliqegnlgl
iravekfdytkgykfstyatwwirqaitramadqartiripvhmvevinklgriqrellq
dlgreptpeelakemditpekvleiqqyarepisldqtigdegdsqlgdfiedseavvav
davsftllqdqlqsvldtlsereagvvrlrfgltdgqprtldeigqvygvtrerirqies
ktmsklrhpsrsqvlrdyl

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6eecJ (J:)
madrvlrgsrlgavsyetdrnhdlaprqiaryrtdngeefevpfaddaeipgtwlcrngm
egtliegdlpepkkvkpprthwdmllerrsieeleellkerlelirsrrrg

    Sequence, based on observed residues (ATOM records):
    >6eecJ (J:)
rvlrgsrlgavsyetdrnhdlaprqiaryrtdngeefevpfaddaeipgtwlcrngmegt
liegdlpepkkvkpprthwdmllerrsieeleellkerlelirsrrrg

  • Chain 'M':
    Sequence, based on SEQRES records:
    >6eecM (M:)
mifkvgdtvvyphhgaalveaietrtikgeqkeylvlkvaqgdltvrvpaenaeyvgvrd
vvgqegldkvfqvlraphteeptnwsrrykanleklasgdvnkvaevvrdlwrrdqergl
sagekrmlakarqilvgelalaestddakaetildevlaaas

    Sequence, based on observed residues (ATOM records):
    >6eecM (M:)
ifkvgdtvvyphhgaalveaietrtikgeqkeylvlkvaqgdltvrvpaenaeyvgvrdv
vgqegldkvfqvlraphteeptnwsrrykanleklasgdvnkvaevvrdlwrrdqergls
agekrmlakarqilvgelalaestddakaetildevlaa

  • Chain 'O':
    No sequence available.

  • Chain 'P':
    No sequence available.