PDB entry 6ee8

View 6ee8 on RCSB PDB site
Description: mycobacterium tuberculosis rnap promoter unwinding intermediate complex with rbpa/card and ap3 promoter
Deposited on 2018-08-13, released 2018-11-21
The last revision was dated 2019-12-18, with a file datestamp of 2019-12-13.
Experiment type: EM
Resolution: 3.92 Å
R-factor: N/A
AEROSPACI score: 0.07 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoA
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoA
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: DNA-directed RNA polymerase subunit beta
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoB, SAMEA2682864_01701
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: DNA-directed RNA polymerase subunit beta'
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoC
    Database cross-references and differences (RAF-indexed):
    • Uniprot A5U053 (2-End)
      • expression tag (0-1)
  • Chain 'E':
    Compound: DNA-directed RNA polymerase subunit omega
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rpoZ, ERS007672_03979, ERS007703_04032, ERS007720_04749, ERS027652_00548, ERS027654_02543, ERS027656_03959, ERS124361_02246
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: RNA polymerase sigma factor SigA
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: sigA, mysA, rpoD, rpoV
    Database cross-references and differences (RAF-indexed):
  • Chain 'J':
    Compound: RNA polymerase-binding protein RbpA
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: rbpA
    Database cross-references and differences (RAF-indexed):
  • Chain 'M':
    Compound: RNA polymerase-binding transcription factor CarD
    Species: Mycobacterium tuberculosis [TaxId:1773]
    Gene: carD
    Database cross-references and differences (RAF-indexed):
  • Chain 'O':
    Compound: DNA (65-mer)
    Species: MYCOBACTERIUM TUBERCULOSIS, synthetic [TaxId:1773]
  • Chain 'P':
    Compound: DNA (60-mer)
    Species: MYCOBACTERIUM TUBERCULOSIS, synthetic [TaxId:1773]
  • Heterogens: ZN, MG

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >6ee8A (A:)
    mlisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvl
    hefttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhn
    pgmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvda
    trveqrtdfdklildvetknsisprdalasagktlvelfglarelnveaegieigpspae
    adhiasfalpiddldltvrsynclkregvhtvgelvartesdlldirnfgqksidevkik
    lhqlglslkdsppsfdpsevagydvatgtwstegaydeqdyaeteql
    

    Sequence, based on observed residues (ATOM records):
    >6ee8A (A:)
    lisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvlh
    efttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhnp
    gmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvdat
    rveqrtdfdklildvetknsisprdalasagktlvelfglareln
    

  • Chain 'B':
    Sequence, based on SEQRES records:
    >6ee8B (B:)
    mlisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvl
    hefttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhn
    pgmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvda
    trveqrtdfdklildvetknsisprdalasagktlvelfglarelnveaegieigpspae
    adhiasfalpiddldltvrsynclkregvhtvgelvartesdlldirnfgqksidevkik
    lhqlglslkdsppsfdpsevagydvatgtwstegaydeqdyaeteql
    

    Sequence, based on observed residues (ATOM records):
    >6ee8B (B:)
    mlisqrptlsedvltdnrsqfvieplepgfgytlgnslrrtllssipgaavtsiridgvl
    hefttvpgvkedvteiilnlkslvvsseedepvtmylrkqgpgevtagdivppagvtvhn
    pgmhiatlndkgklevelvvergrgyvpavqnrasgaeigripvdsiyspvlkvtykvda
    trveqrtdfdklildvetknsisprdalasagktlvelfglarelnveaegieigps
    

  • Chain 'C':
    Sequence, based on SEQRES records:
    >6ee8C (C:)
    madsrqsktaaspspsrpqsssnnsvpgapnrvsfaklreplevpglldvqtdsfewlig
    sprwresaaergdvnpvggleevlyelspiedfsgsmslsfsdprfddvkapvdeckdkd
    mtyaaplfvtaefinnntgeiksqtvfmgdfpmmtekgtfiingtervvvsqlvrspgvy
    fdetidkstdktlhsvkvipsrgawlefdvdkrdtvgvridrkrrqpvtvllkalgwtse
    qiverfgfseimrstlekdntvgtdealldiyrklrpgepptkesaqtllenlffkekry
    dlarvgrykvnkklglhvgepitsstlteedvvatieylvrlhegqttmtvpggvevpve
    tddidhfgnrrlrtvgeliqnqirvgmsrmervvrermttqdveaitpqtlinirpvvaa
    ikeffgtsqlsqfmdqnnplsglthkrrlsalgpgglsreraglevrdvhpshygrmcpi
    etpegpnigligslsvyarvnpfgfietpyrkvvdgvvsdeivyltadeedrhvvaqans
    pidadgrfveprvlvrrkageveyvpssevdymdvsprqmvsvatamipflehddanral
    mganmqrqavplvrseaplvgtgmelraaidagdvvvaeesgvieevsadyitvmhdngt
    rrtyrmrkfarsnhgtcanqcpivdagdrveagqviadgpctddgemalgknllvaimpw
    eghnyedaiilsnrlveedvltsihieeheidardtklgaeeitrdipnisdevladlde
    rgivrigaevrdgdilvgkvtpkgeteltpeerllraifgekarevrdtslkvphgesgk
    vigirvfsrededelpagvnelvrvyvaqkrkisdgdklagrhgnkgvigkilpvedmpf
    ladgtpvdiilnthgvprrmnigqilethlgwcahsgwkvdaakgvpdwaarlpdellea
    qpnaivstpvfdgaqeaelqgllsctlpnrdgdvlvdadgkamlfdgrsgepfpypvtvg
    ymyimklhhlvddkiharstgpysmitqqplggkaqfggqrfgemecwamqaygaaytlq
    elltiksddtvgrvkvyeaivkgenipepgipesfkvllkelqslclnvevlss
    

    Sequence, based on observed residues (ATOM records):
    >6ee8C (C:)
    nsvpgapnrvsfaklreplevpglldvqtdsfewligsprwresaaergdvnpvggleev
    lyelspiedfsgsmslsfsdprfddvkapvdeckdkdmtyaaplfvtaefinnntgeiks
    qtvfmgdfpmmtekgtfiingtervvvsqlvrspgvyfdetidkstdktlhsvkvipsrg
    awlefdvdkrdtvgvridrkrrqpvtvllkalgwtseqiverfgfseimrstlekdntvg
    tdealldiyrklrpgepptkesaqtllenlffkekrydlarvgrykvnkklglhvgepit
    sstlteedvvatieylvrlhegqttmtvpggvevpvetddidhfgnrrlrtvgeliqnqi
    rvgmsrmervvrermttqdveaitpqtlinirpvvaaikeffgtsqlsqfmdqnnplsgl
    thkrrlsalgpgglsreraglevrdvhpshygrmcpietpegpnigligslsvyarvnpf
    gfietpyrkvvdgvvsdeivyltadeedrhvvaqanspidadgrfveprvlvrrkageve
    yvpssevdymdvsprqmvsvatamipflehddanralmganmqrqavplvrseaplvgtg
    melraaidagdvvvaeesgvieevsadyitvmhdngtrrtyrmrkfarsnhgtcanqcpi
    vdagdrveagqviadgpctddgemalgknllvaimpweghnyedaiilsnrlveedvlts
    ihieeheidardtklgaeeitrdipnisdevladldergivrigaevrdgdilvgkvtpk
    geteltpeerllraifgekarevrdtslkvphgesgkvigirvfsrededelpagvnelv
    rvyvaqkrkisdgdklagrhgnkgvigkilpvedmpfladgtpvdiilnthgvprrmnig
    qilethlgwcahsgwkvdaakgvpdwaarlpdelleaqpnaivstpvfdgaqeaelqgll
    sctlpnrdgdvlvdadgkamlfdgrsgepfpypvtvgymyimklhhlvddkiharstgpy
    smitqqplggkaqfggqrfgemecwamqaygaaytlqelltiksddtvgrvkvyeaivkg
    enipepgipesfkvllkelqslclnvevlss
    

  • Chain 'D':
    Sequence, based on SEQRES records:
    >6ee8D (D:)
    gamldvnffdelriglataedirqwsygevkkpetinyrtlkpekdglfcekifgptrdw
    ecycgkykrvrfkgiicercgvevtrakvrrermghielaapvthiwyfkgvpsrlgyll
    dlapkdlekiiyfaayvitsvdeemrhnelstleaemaverkavedqrdgelearaqkle
    adlaeleaegakadarrkvrdggeremrqirdraqreldrlediwstftklapkqlivde
    nlyrelvdrygeyftgamgaesiqklienfdidaeaeslrdvirngkgqkklralkrlkv
    vaafqqsgnspmgmvldavpvippelrpmvqldggrfatsdlndlyrrvinrnnrlkrli
    dlgapeiivnnekrmlqesvdalfdngrrgrpvtgpgnrplkslsdllkgkqgrfrqnll
    gkrvdysgrsvivvgpqlklhqcglpklmalelfkpfvmkrlvdlnhaqniksakrmver
    qrpqvwdvleeviaehpvllnraptlhrlgiqafepmlvegkaiqlhplvceafnadfdg
    dqmavhlplsaeaqaearilmlssnnilspasgrplamprldmvtglyylttevpgdtge
    yqpasgdhpetgvysspaeaimaadrgvlsvrakikvrltqlrppveieaelfghsgwqp
    gdawmaettlgrvmfnellplgypfvnkqmhkkvqaaiindlaerypmivvaqtvdklkd
    agfywatrsgvtvsmadvlvpprkkeildhyeeradkvekqfqrgalnhdernealveiw
    keatdevgqalrehypddnpiitivdsgatgnftqtrtlagmkglvtnpkgefiprpvks
    sfregltvleyfinthgarkgladtalrtadsgyltrrlvdvsqdvivrehdcqtergiv
    velaerapdgtlirdpyietsayartlgtdavdeagnvivergqdlgdpeidallaagit
    qvkvrsvltcatstgvcatcygrsmatgklvdigeavgivaaqsigepgtqltmrtfhqg
    gvgeditgglprvqelfearvprgkapiadvtgrvrledgerfykitivpddggeevvyd
    kiskrqrlrvfkhedgservlsdgdhvevgqqlmegsadphevlrvqgprevqihlvrev
    qevyraqgvsihdkhievivrqmlrrvtiidsgsteflpgslidraefeaenrrvvaegg
    epaagrpvlmgitkaslatdswlsaasfqettrvltdaaincrsdklnglkenviigkli
    pagtginryrniavqpteearaaaytipsyedqyyspdfgaatgaavplddygysdyrhh
    hhhhhh
    

    Sequence, based on observed residues (ATOM records):
    >6ee8D (D:)
    gamldvnffdelriglataedirqwsygevkkpetinyrtlkpekdglfcekifgptrdw
    ecycgkykrvrfkgiicercgvevtrakvrrermghielaapvthiwyfkgvpsrlgyll
    dlapkdlekiiyfaayvitsvdeemrhnelstleaemaverkavedqrdgelearaqkle
    adlaeleaegakadarrkvrdggeremrqirdraqreldrlediwstftklapkqlivde
    nlyrelvdrygeyftgamgaesiqklienfdidaeaeslrdvirngkgqkklralkrlkv
    vaafqqsgnspmgmvldavpvippelrpmvqldggrfatsdlndlyrrvinrnnrlkrli
    dlgapeiivnnekrmlqesvdalfdngrrgrpvtgpgnrplkslsdllkgkqgrfrqnll
    gkrvdysgrsvivvgpqlklhqcglpklmalelfkpfvmkrlvdlnhaqniksakrmver
    qrpqvwdvleeviaehpvllnraptlhrlgiqafepmlvegkaiqlhplvceafnadfdg
    dqmavhlplsaeaqaearilmlssnnilspasgrplamprldmvtglyylttevpgdtge
    yqpasgdhpetgvysspaeaimaadrgvlsvrakikvrltqlrppveieaelfghsgwqp
    gdawmaettlgrvmfnellplgypfvnkqmhkkvqaaiindlaerypmivvaqtvdklkd
    agfywatrsgvtvsmadvlvpprkkeildhyeeradkvekqfqrgalnhdernealveiw
    keatdevgqalrehypddnpiitivdsgatgnftqtrtlagmkglvtnpkgefiprpvks
    sfregltvleyfinthgarkgladtalrtadsgyltrrlvdvsqdvivrehdcqtergiv
    velaerapdgtlirdpyietsayartlgtdavdeagnvivergqdlgdpeidallaagit
    qvkvrsvltcatstgvcatcygrsmatgklvdigeavgivaaqsigepgtqltmtgglpr
    vqelfearvprgkapiadvtgrvrledgerfykitivpddggeevvydkiskrqrlrvfk
    rvlsdgdhvevgqqlmegsadphevlrvqgprevqihlvrevqevyraqgvsihdkhiev
    ivrqmlrrvtiidsgsteflpgslidraefeaenrrvvaeggepaagrpvlmgitkasla
    tdswlsaasfqettrvltdaaincrsdklnglkenviigklipagtginryrniavqpte
    earaaa
    

  • Chain 'E':
    Sequence, based on SEQRES records:
    >6ee8E (E:)
    gsisqsdaslaavpavdqfdpssgasggydtplgitnppidelldrvsskyalviyaakr
    arqindyynqlgegileyvgplvepglqekplsialreihadllehtege
    

    Sequence, based on observed residues (ATOM records):
    >6ee8E (E:)
    ggydtplgitnppidelldrvsskyalviyaakrarqindyynqlgegileyvgplvepg
    lqekplsialreihadllehteg
    

  • Chain 'F':
    Sequence, based on SEQRES records:
    >6ee8F (F:)
    gphmaatkastatdepvkrtatkspaasasgaktgakrtaaksasgsppakratkpaars
    vkpasapqdtttstipkrktraaaksaaakapsarghatkprapkdaqheaatdpedald
    sveeldaepdldvepgedldldaadlnlddleddvapdadddldsgddedhedleaeaav
    apgqtadddeeiaeptekdkasgdfvwdedesealrqarkdaeltasadsvraylkqigk
    vallnaeeevelakrieaglyatqlmtelsergeklpaaqrrdmmwicrdgdraknhlle
    anlrlvvslakrytgrgmafldliqegnlgliravekfdytkgykfstyatwwirqaitr
    amadqartiripvhmvevinklgriqrellqdlgreptpeelakemditpekvleiqqya
    repisldqtigdegdsqlgdfiedseavvavdavsftllqdqlqsvldtlsereagvvrl
    rfgltdgqprtldeigqvygvtrerirqiesktmsklrhpsrsqvlrdyld
    

    Sequence, based on observed residues (ATOM records):
    >6ee8F (F:)
    sealrqarkdaeltasadsvraylkqigkvallnaeeevelakrieaglyatqlmtelse
    rgeklpaaqrrdmmwicrdgdraknhlleanlrlvvslakrytgrgmafldliqegnlgl
    iravekfdytkgykfstyatwwirqaitramadqartiripvhmvevinklgriqrellq
    dlgreptpeelakemditpekvleiqqyarepisldqtigdegdsqlgdfiedseavvav
    davsftllqdqlqsvldtlsereagvvrlrfgltdgqprtldeigqvygvtrerirqies
    ktmsklrhpsrsqvlrdyl
    

  • Chain 'J':
    Sequence, based on SEQRES records:
    >6ee8J (J:)
    madrvlrgsrlgavsyetdrnhdlaprqiaryrtdngeefevpfaddaeipgtwlcrngm
    egtliegdlpepkkvkpprthwdmllerrsieeleellkerlelirsrrrg
    

    Sequence, based on observed residues (ATOM records):
    >6ee8J (J:)
    rvlrgsrlgavsyetdrnhdlaprqiaryrtdngeefevpfaddaeipgtwlcrngmegt
    liegdlpepkkvkpprthwdmllerrsieeleellkerlelirsrrrg
    

  • Chain 'M':
    Sequence, based on SEQRES records:
    >6ee8M (M:)
    mifkvgdtvvyphhgaalveaietrtikgeqkeylvlkvaqgdltvrvpaenaeyvgvrd
    vvgqegldkvfqvlraphteeptnwsrrykanleklasgdvnkvaevvrdlwrrdqergl
    sagekrmlakarqilvgelalaestddakaetildevlaaas
    

    Sequence, based on observed residues (ATOM records):
    >6ee8M (M:)
    ifkvgdtvvyphhgaalveaietrtikgeqkeylvlkvaqgdltvrvpaenaeyvgvrdv
    vgqegldkvfqvlraphteeptnwsrrykanleklasgdvnkvaevvrdlwrrdqergls
    agekrmlakarqilvgelalaestddakaetildevlaa
    

  • Chain 'O':
    No sequence available.

  • Chain 'P':
    No sequence available.