PDB entry 6at2

View 6at2 on RCSB PDB site
Description: E. coli phosphoenolpyruvate carboxykinase G209N mutant bound to thiosulfate
Class: lyase
Keywords: Nonnative ligand, LYASE
Deposited on 2017-08-27, released 2018-08-29
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-12-04, with a file datestamp of 2019-11-29.
Experiment type: XRAY
Resolution: 1.44 Å
R-factor: N/A
AEROSPACI score: 0.51 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Phosphoenolpyruvate carboxykinase (ATP)
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: pckA, pck, b3403, JW3366
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22259 (Start-539)
      • engineered mutation (208)
      • expression tag (540-541)
    Domains in SCOPe 2.08: d6at2a1, d6at2a2, d6at2a3
  • Heterogens: THJ, ATP, MN, MG, TRS, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >6at2A (A:)
    mrvnngltpqeleaygisdvhdivynpsydllyqeeldpsltgyergvltnlgavavdtg
    iftgrspkdkyivrddttrdtfwwadkgkgkndnkplspetwqhlkglvtrqlsgkrlfv
    vdafcganpdtrlsvrfitevawqahfvknmfirpsdeelagfkpdfivmngakctnpqw
    keqglnsenfvafnltermqliggtwygnemkkgmfsmmnyllplkgiasmhcsanvgek
    gdvavffglsgtgkttlstdpkrrligddehgwdddgvfnfeggcyaktiklskeaepei
    ynairrdallenvtvredgtidfddgsktentrvsypiyhidnivkpvskaghatkvifl
    tadafgvlppvsrltadqtqyhflsgftaklagtergiteptptfsacfgaaflslhptq
    yaevlvkrmqaagaqaylvntgwngtgkrisikdtraiidailngsldnaetftlpmfnl
    aiptelpgvdtkildprntyaspeqwqekaetlaklfidnfdkytdtpagaalvaagpkl
    hhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >6at2A (A:)
    gltpqeleaygisdvhdivynpsydllyqeeldpsltgyergvltnlgavavdtgiftgr
    spkdkyivrddttrdtfwwadkgkndnkplspetwqhlkglvtrqlsgkrlfvvdafcga
    npdtrlsvrfitevawqahfvknmfirpsdeelagfkpdfivmngakctnpqwkeqglns
    enfvafnltermqliggtwygnemkkgmfsmmnyllplkgiasmhcsanvgekgdvavff
    glsgtgkttlstdpkrrligddehgwdddgvfnfeggcyaktiklskeaepeiynairrd
    allenvtvredgtidfddgsktentrvsypiyhidnivkpvskaghatkvifltadafgv
    lppvsrltadqtqyhflsgftaklagtergiteptptfsacfgaaflslhptqyaevlvk
    rmqaagaqaylvntgwngtgkrisikdtraiidailngsldnaetftlpmfnlaiptelp
    gvdtkildprntyaspeqwqekaetlaklfidnfdkytdtpagaalvaagpklhh