PDB entry 5z0t

View 5z0t on RCSB PDB site
Description: Thermoactinomyces vulgaris R-47 alpha-amylase I (TVA I) mutant A357V/Q359N/Y360E (AQY/VNE)
Class: hydrolase
Keywords: alpha-amylase, neopullulanase, GH13, HYDROLASE
Deposited on 2017-12-21, released 2018-02-21
The last revision prior to the SCOPe 2.07 freeze date was dated 2018-03-28, with a file datestamp of 2018-03-23.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: N/A
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Neopullulanase 1
    Species: Thermoactinomyces vulgaris [TaxId:2026]
    Gene: tvaI
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q60053 (0-636)
      • engineered mutation (356)
      • engineered mutation (358-359)
    Domains in SCOPe 2.07: d5z0ta1, d5z0ta2, d5z0ta3
  • Chain 'B':
    Compound: Neopullulanase 1
    Species: Thermoactinomyces vulgaris [TaxId:2026]
    Gene: tvaI
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q60053 (0-636)
      • engineered mutation (356)
      • engineered mutation (358-359)
    Domains in SCOPe 2.07: d5z0tb1, d5z0tb2, d5z0tb3
  • Heterogens: CA, MPD, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5z0tA (A:)
aandnnvewnglfhdqgplfdnapeptstqsvtlklrtfkgditsanikywdtadnafhw
vpmvwdsndptgtfdywkgtipaspsikyyrfqindgtstawyngngpsstepnaddfyi
ipnfktpdwlkngvmyqifpdrfyngdssndvqtgsytyngtptekkawgssvyadpgyd
nslvffggdlagidqklgyikktlganilylnpifkaptnhkydtqdymavdpafgdnst
lqtlindihstangpkgylildgvfnhtgdshpwfdkynnfssqgayesqsspwynyytf
ytwpdsyasflgfnslpklnygnsgsavrgviynnsnsvaktylnppysvdgwrldvane
vdangnngsdvtnhqiwsefrnavkgvnsnaaiigeywgnanpwtaqgnqwdaatnfdgf
tqpvsewitgkdyqnnsasisttqfdswlrgtranyptnvqqsmmnflsnhditrfatrs
ggdlwktylalifqmtyvgtptiyygdeygmqggadpdnrrsfdwsqatpsnsavaltqk
litirnqypalrtgsfmtlitddtnkiysygrfdnvnriavvlnndsvshtvnvpvwqls
mpngstvtdkitghsytvqngmvtvavdghygavlaq

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5z0tB (B:)
aandnnvewnglfhdqgplfdnapeptstqsvtlklrtfkgditsanikywdtadnafhw
vpmvwdsndptgtfdywkgtipaspsikyyrfqindgtstawyngngpsstepnaddfyi
ipnfktpdwlkngvmyqifpdrfyngdssndvqtgsytyngtptekkawgssvyadpgyd
nslvffggdlagidqklgyikktlganilylnpifkaptnhkydtqdymavdpafgdnst
lqtlindihstangpkgylildgvfnhtgdshpwfdkynnfssqgayesqsspwynyytf
ytwpdsyasflgfnslpklnygnsgsavrgviynnsnsvaktylnppysvdgwrldvane
vdangnngsdvtnhqiwsefrnavkgvnsnaaiigeywgnanpwtaqgnqwdaatnfdgf
tqpvsewitgkdyqnnsasisttqfdswlrgtranyptnvqqsmmnflsnhditrfatrs
ggdlwktylalifqmtyvgtptiyygdeygmqggadpdnrrsfdwsqatpsnsavaltqk
litirnqypalrtgsfmtlitddtnkiysygrfdnvnriavvlnndsvshtvnvpvwqls
mpngstvtdkitghsytvqngmvtvavdghygavlaq