PDB entry 5usr

View 5usr on RCSB PDB site
Description: Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms
Class: transferase
Keywords: LYR, Fe-S cluster assembly, NFS1, acyl-carrier protein, TRANSFERASE
Deposited on 2017-02-13, released 2017-06-21
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-11-27, with a file datestamp of 2019-11-22.
Experiment type: XRAY
Resolution: 3.09 Å
R-factor: N/A
AEROSPACI score: 0.14 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Cysteine desulfurase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: NFS1, NIFS, HUSSY-08
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9Y697 (24-End)
      • expression tag (22-23)
    Domains in SCOPe 2.08: d5usra1, d5usra2
  • Chain 'B':
    Compound: LYR motif-containing protein 4
    Species: Homo sapiens [TaxId:9606]
    Gene: LYRM4, C6orf149, ISD11, CGI-203
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9HD34
      • engineered mutation (10)
    Domains in SCOPe 2.08: d5usrb_
  • Chain 'C':
    Compound: Cysteine desulfurase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: NFS1, NIFS, HUSSY-08
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5usrc_
  • Chain 'D':
    Compound: LYR motif-containing protein 4
    Species: Homo sapiens [TaxId:9606]
    Gene: LYRM4, C6orf149, ISD11, CGI-203
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9HD34
      • engineered mutation (10)
    Domains in SCOPe 2.08: d5usrd_
  • Chain 'E':
    Compound: Cysteine desulfurase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: NFS1, NIFS, HUSSY-08
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9Y697 (24-End)
      • expression tag (15-23)
    Domains in SCOPe 2.08: d5usre1, d5usre2
  • Chain 'F':
    Compound: LYR motif-containing protein 4
    Species: Homo sapiens [TaxId:9606]
    Gene: LYRM4, C6orf149, ISD11, CGI-203
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9HD34
      • engineered mutation (10)
    Domains in SCOPe 2.08: d5usrf_
  • Chain 'G':
    Compound: Cysteine desulfurase, mitochondrial
    Species: Homo sapiens [TaxId:9606]
    Gene: NFS1, NIFS, HUSSY-08
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9Y697 (24-End)
      • expression tag (22-23)
    Domains in SCOPe 2.08: d5usrg1, d5usrg2
  • Chain 'H':
    Compound: LYR motif-containing protein 4
    Species: Homo sapiens [TaxId:9606]
    Gene: LYRM4, C6orf149, ISD11, CGI-203
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9HD34
      • engineered mutation (10)
    Domains in SCOPe 2.08: d5usrh_
  • Chain 'I':
    Compound: Acyl carrier protein
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5usri_
  • Chain 'J':
    Compound: Acyl carrier protein
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5usrj_
  • Chain 'K':
    Compound: Acyl carrier protein
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5usrk_
  • Chain 'L':
    Compound: Acyl carrier protein
    Species: Escherichia coli (strain K12) [TaxId:83333]
    Gene: acpP, b1094, JW1080
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d5usrl_
  • Heterogens: 8Q1

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5usrA (A:)
mgsshhhhhhssglvprgshmlemlrplymdvqattpldprvldamlpylinyygnphsr
thaygweseaamerarqqvasligadpreiiftsgatesnniaikgvarfyrsrkkhlit
tqtehkcvldscrsleaegfqvtylpvqksgiidlkeleaaiqpdtslvsvmtvnneigv
kqpiaeigricssrkvyfhtdaaqavgkipldvndmkidlmsisghkiygpkgvgaiyir
rrprvrvealqsgggqergmrsgtvptplvvglgaacevaqqemeydhkrisklserliq
nimkslpdvvmngdpkhhypgcinlsfayvegesllmalkdvalssgsactsaslepsyv
lraigtdedlahssirfgigrftteeevdytvekciqhvkrlremsplwemvqdgidlks
ikwtqh

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrA (A:)
emlrplymdvqattpldprvldamlpylinyygnphsrthaygweseaamerarqqvasl
igadpreiiftsgatesnniaikgvarfyrsrkkhlittqtehkcvldscrsleaegfqv
tylpvqksgiidlkeleaaiqpdtslvsvmtvnneigvkqpiaeigricssrkvyfhtda
aqavgkipldvndmkidlmsisghkiygpkgvgaiyirrrprvrvealqgtvptplvvgl
gaacevaqqemeydhkrisklserliqnimkslpdvvmngdpkhhypgcinlsfayvege
sllslepsyvlraigtdedlahssirfgigrftteeevdytvekciqhvkrlremsplwe
mvqd

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5usrB (B:)
maassraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkak
rdlgvirrqvhigqlystdkliienrdmprt

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrB (B:)
ssraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkakrdl
gvirrqvhigqlystd

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5usrC (C:)
mgsshhhhhhssglvprgshmlemlrplymdvqattpldprvldamlpylinyygnphsr
thaygweseaamerarqqvasligadpreiiftsgatesnniaikgvarfyrsrkkhlit
tqtehkcvldscrsleaegfqvtylpvqksgiidlkeleaaiqpdtslvsvmtvnneigv
kqpiaeigricssrkvyfhtdaaqavgkipldvndmkidlmsisghkiygpkgvgaiyir
rrprvrvealqsgggqergmrsgtvptplvvglgaacevaqqemeydhkrisklserliq
nimkslpdvvmngdpkhhypgcinlsfayvegesllmalkdvalssgsactsaslepsyv
lraigtdedlahssirfgigrftteeevdytvekciqhvkrlremsplwemvqdgidlks
ikwtqh

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrC (C:)
lrplymdvqattpldprvldamlpylinyyggweseaamerarqqvasligadpreiift
sgatesnniaikgvarfyrsrkkhlittqtehkcvldscrsleaegfqvtylpvqksgii
dlkeleaaiqpdtslvsvmtvnneigvkqpiaeigricssrkvyfhtdaaqavgkipldv
ndmkidlmsisghkiygpkgvgaiyirrrprvrvealqtvptplvvglgaacevaqqeme
ydhkrisklserliqnimkslpdvvmngdpkhhypgcinlsfayvegesllmalkdsyvl
raigtdedlahssirfgigrftteeevdytvekciqhvkrlremsplwemvq

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5usrD (D:)
maassraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkak
rdlgvirrqvhigqlystdkliienrdmprt

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrD (D:)
ssraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkakrdl
gvirrqvhigqlystdklii

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >5usrE (E:)
mgsshhhhhhssglvprgshmlemlrplymdvqattpldprvldamlpylinyygnphsr
thaygweseaamerarqqvasligadpreiiftsgatesnniaikgvarfyrsrkkhlit
tqtehkcvldscrsleaegfqvtylpvqksgiidlkeleaaiqpdtslvsvmtvnneigv
kqpiaeigricssrkvyfhtdaaqavgkipldvndmkidlmsisghkiygpkgvgaiyir
rrprvrvealqsgggqergmrsgtvptplvvglgaacevaqqemeydhkrisklserliq
nimkslpdvvmngdpkhhypgcinlsfayvegesllmalkdvalssgsactsaslepsyv
lraigtdedlahssirfgigrftteeevdytvekciqhvkrlremsplwemvqdgidlks
ikwtqh

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrE (E:)
prgshmlemlrplymdvqattpldprvldamlpylinyygygweseaamerarqqvasli
gadpreiiftsgatesnniaikgvarfyrsrkkhlittqtehkcvldscrsleaegfqvt
ylpvqksgiidlkeleaaiqpdtslvsvmtvnneigvkqpiaeigricssrkvyfhtdaa
qavgkipldvndmkidlmsisghkiygpkgvgaiyirrrprtvptplvvglgaacevaqq
emeydhkrisklserliqnimkslpdvvmngdpkhhypgcinlsfaahssirfgigrftt
eeevdytvekciqhvkrlrem

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >5usrF (F:)
maassraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkak
rdlgvirrqvhigqlystdkliienrdmprt

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrF (F:)
ssraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkakrdl
gvirrqvhigqlyst

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >5usrG (G:)
mgsshhhhhhssglvprgshmlemlrplymdvqattpldprvldamlpylinyygnphsr
thaygweseaamerarqqvasligadpreiiftsgatesnniaikgvarfyrsrkkhlit
tqtehkcvldscrsleaegfqvtylpvqksgiidlkeleaaiqpdtslvsvmtvnneigv
kqpiaeigricssrkvyfhtdaaqavgkipldvndmkidlmsisghkiygpkgvgaiyir
rrprvrvealqsgggqergmrsgtvptplvvglgaacevaqqemeydhkrisklserliq
nimkslpdvvmngdpkhhypgcinlsfayvegesllmalkdvalssgsactsaslepsyv
lraigtdedlahssirfgigrftteeevdytvekciqhvkrlremsplwemvqdgidlks
ikwtqh

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrG (G:)
emlrplyttpldprvldamlpylinyygeseaamerarqqvasligadpreiiftsgate
snniaikgvarfyrsrkkhlittqtehkcvldscrsleaegfqvtylpvqksgiidlkel
eaaiqpdtslvsvmtvnneigvkqpiaeigricssrkvyfhtdaaqavgkipldvndmki
dlmsisghkiygpkgvgaiyirrrprtvptplvvglgaacevaqqemeydhkrisklser
liqnimkslpdvvmngdpkhhypgcinlsfayvegeslhssirfgigrftteeevdytve
kciqhvkrlremsplw

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >5usrH (H:)
maassraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkak
rdlgvirrqvhigqlystdkliienrdmprt

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrH (H:)
assraqvlalyramlreskrfsaynyrtyavrrirdafrenknvkdpveiqtlvnkakrd
lgvirrqvhigqlystd

  • Chain 'I':
    Sequence, based on SEQRES records: (download) 
    >5usrI (I:)
mstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeea
ekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrI (I:)
stieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeeae
kittvqaaidyinghq

  • Chain 'J':
    Sequence, based on SEQRES records: (download) 
    >5usrJ (J:)
mstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeea
ekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrJ (J:)
tieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeeaek
ittvqaaidyinghq

  • Chain 'K':
    Sequence, based on SEQRES records: (download) 
    >5usrK (K:)
mstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeea
ekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrK (K:)
eervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeeaekit
tvqaaidyi

  • Chain 'L':
    Sequence, based on SEQRES records: (download) 
    >5usrL (L:)
mstieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeea
ekittvqaaidyinghqa

    Sequence, based on observed residues (ATOM records): (download) 
    >5usrL (L:)
tieervkkiigeqlgvkqeevtnnasfvedlgadsldtvelvmaleeefdteipdeeaek
ittvqaaidyi