PDB entry 5tot

View 5tot on RCSB PDB site
Description: Crystal structure of AAT H143L:H189L double mutant
Class: transferase
Keywords: transferase
Deposited on 2016-10-18, released 2017-03-01
The last revision prior to the SCOPe 2.06 freeze date was dated 2017-03-01, with a file datestamp of 2017-02-24.
Experiment type: XRAY
Resolution: 1.4 Å
R-factor: N/A
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Aspartate aminotransferase, cytoplasmic
    Species: Sus scrofa [TaxId:9823]
    Gene: GOT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00503 (1-413)
      • expression tag (0)
      • conflict (64)
      • engineered mutation (144)
      • engineered mutation (190)
      • conflict (289)
      • conflict (377)
    Domains in SCOPe 2.06: d5tota1, d5tota2
  • Chain 'B':
    Compound: Aspartate aminotransferase, cytoplasmic
    Species: Sus scrofa [TaxId:9823]
    Gene: GOT1
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00503 (1-End)
      • expression tag (0)
      • conflict (64)
      • engineered mutation (144)
      • engineered mutation (190)
      • conflict (289)
      • conflict (377)
    Domains in SCOPe 2.06: d5totb1, d5totb2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5totA (A:)
gmappsvfaevpqaqpvlvfkliadfredpdprkvnlgvgayrtddcqpwvlpvvrkveq
riannsslnheylpilglaefrtcasrlalgddspalqekrvggvqslggtgalrigaef
larwyngtnnkdtpvyvssptwenlngvfttagfkdirsyrywdtekrgldlqgflsdle
napefsifvllacahnptgtdptpeqwkqiasvmkrrflfpffdsayqgfasgnlekdaw
airyfvsegfelfcaqsfsknfglynervgnltvvakepdsilrvlsqmqkivrvtwsnp
paqgarivartlsdpelfhewtgnvktmadrilsmrselrarlealktpgtwnhitdqig
mfsftglnpkqveylinqkhiyllpsgrinmcglttknldyvatsiheavtkiq

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5totB (B:)
gmappsvfaevpqaqpvlvfkliadfredpdprkvnlgvgayrtddcqpwvlpvvrkveq
riannsslnheylpilglaefrtcasrlalgddspalqekrvggvqslggtgalrigaef
larwyngtnnkdtpvyvssptwenlngvfttagfkdirsyrywdtekrgldlqgflsdle
napefsifvllacahnptgtdptpeqwkqiasvmkrrflfpffdsayqgfasgnlekdaw
airyfvsegfelfcaqsfsknfglynervgnltvvakepdsilrvlsqmqkivrvtwsnp
paqgarivartlsdpelfhewtgnvktmadrilsmrselrarlealktpgtwnhitdqig
mfsftglnpkqveylinqkhiyllpsgrinmcglttknldyvatsiheavtkiq

    Sequence, based on observed residues (ATOM records): (download) 
    >5totB (B:)
gmappsvfaevpqaqprkvnlgvgayrtddcqpwvlpvvrkveqriannsslnheylpil
glaefrtcasrlalgddspalqekrvggvqslggtgalrigaeflarwyngtnnkdtpvy
vssptwenlngvfttagfkdirsyrywdtekrgldlqgflsdlenapefsifvllacahn
ptgtdptpeqwkqiasvmkrrflfpffdsayqgfasgnlekdawairyfvsegfelfcaq
sfsknfglynervgnltvvakepdsilrvlsqmqkivrvtwsnppaqgarivartlsdpe
lfhewtgnvktmadrilsmrselrarlealktpgtwnhitdqigmfsftglnpkqveyli
nqkhiyllpsgrinmcglttknldyvatsiheavtki