PDB entry 5oke

View 5oke on RCSB PDB site
Description: Conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with cellobiose-6-phosphate
Class: hydrolase
Keywords: 6-phospho-beta-galactosidase, Geobacillus stearothermophilus, Glycoside Hydrolase, GH1 family, HYDROLASE
Deposited on 2017-07-25, released 2017-10-18
The last revision prior to the SCOPe 2.06 freeze date was dated 2017-10-25, with a file datestamp of 2017-10-20.
Experiment type: XRAY
Resolution: 1.31 Å
R-factor: N/A
AEROSPACI score: 0.58 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Putative 6-phospho-beta-galactobiosidase
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: gan1D
    Database cross-references and differences (RAF-indexed):
    • Uniprot W8QF82 (Start-484)
      • engineered mutation (176)
    Domains in SCOPe 2.06: d5okea_
  • Chain 'B':
    Compound: Putative 6-phospho-beta-galactobiosidase
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: gan1D
    Database cross-references and differences (RAF-indexed):
    • Uniprot W8QF82 (Start-484)
      • engineered mutation (176)
    Domains in SCOPe 2.06: d5okeb_
  • Chain 'C':
    Compound: Putative 6-phospho-beta-galactobiosidase
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: gan1D
    Database cross-references and differences (RAF-indexed):
    • Uniprot W8QF82 (Start-484)
      • engineered mutation (176)
    Domains in SCOPe 2.06: d5okec_
  • Chain 'D':
    Compound: Putative 6-phospho-beta-galactobiosidase
    Species: Geobacillus stearothermophilus [TaxId:1422]
    Gene: gan1D
    Database cross-references and differences (RAF-indexed):
    • Uniprot W8QF82 (Start-484)
      • engineered mutation (176)
    Domains in SCOPe 2.06: d5oked_
  • Heterogens: BG6, GOL, IMD, XYT, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5okeA (A:)
mihhhhhhehrhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtn
gdvavdhyhryqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrn
hgiepivtlyhwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqni
fisfgyrlglhppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypy
dsrpenvlafenaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakp
dfmgvnyyqtttvehnppdgvgegvmnttgkkgtstssgipglfktvrnphvdttnwdwa
idpvglriglrrianryqlpilitenglgefdtlepgdivnddyridylrrhvqeiqrai
tdgvdvlgycawsftdllswlngyqkrygfvyvnrddesekdlrrikkksfywyqrviet
ngael

    Sequence, based on observed residues (ATOM records): (download) 
    >5okeA (A:)
rhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtngdvavdhyhr
yqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrnhgiepivtly
hwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqnifisfgyrlgl
hppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypydsrpenvlaf
enaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakpdfmgvnyyqt
ttvehnppdgvgtssgipglfktvrnphvdttnwdwaidpvglriglrrianryqlpili
tenglgefdtlepgdivnddyridylrrhvqeiqraitdgvdvlgycawsftdllswlng
yqkrygfvyvnrddesekdlrrikkksfywyqrvietngael

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5okeB (B:)
mihhhhhhehrhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtn
gdvavdhyhryqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrn
hgiepivtlyhwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqni
fisfgyrlglhppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypy
dsrpenvlafenaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakp
dfmgvnyyqtttvehnppdgvgegvmnttgkkgtstssgipglfktvrnphvdttnwdwa
idpvglriglrrianryqlpilitenglgefdtlepgdivnddyridylrrhvqeiqrai
tdgvdvlgycawsftdllswlngyqkrygfvyvnrddesekdlrrikkksfywyqrviet
ngael

    Sequence, based on observed residues (ATOM records): (download) 
    >5okeB (B:)
rhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtngdvavdhyhr
yqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrnhgiepivtly
hwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqnifisfgyrlgl
hppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypydsrpenvlaf
enaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakpdfmgvnyyqt
ttvehnppdgvgtssgipglfktvrnphvdttnwdwaidpvglriglrrianryqlpili
tenglgefdtlepgdivnddyridylrrhvqeiqraitdgvdvlgycawsftdllswlng
yqkrygfvyvnrddesekdlrrikkksfywyqrvietngael

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5okeC (C:)
mihhhhhhehrhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtn
gdvavdhyhryqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrn
hgiepivtlyhwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqni
fisfgyrlglhppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypy
dsrpenvlafenaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakp
dfmgvnyyqtttvehnppdgvgegvmnttgkkgtstssgipglfktvrnphvdttnwdwa
idpvglriglrrianryqlpilitenglgefdtlepgdivnddyridylrrhvqeiqrai
tdgvdvlgycawsftdllswlngyqkrygfvyvnrddesekdlrrikkksfywyqrviet
ngael

    Sequence, based on observed residues (ATOM records): (download) 
    >5okeC (C:)
rhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtngdvavdhyhr
yqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrnhgiepivtly
hwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqnifisfgyrlgl
hppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypydsrpenvlaf
enaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakpdfmgvnyyqt
ttvehnppdgvgtssgipglfktvrnphvdttnwdwaidpvglriglrrianryqlpili
tenglgefdtlepgdivnddyridylrrhvqeiqraitdgvdvlgycawsftdllswlng
yqkrygfvyvnrddesekdlrrikkksfywyqrvietngael

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5okeD (D:)
mihhhhhhehrhlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtn
gdvavdhyhryqedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrn
hgiepivtlyhwdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqni
fisfgyrlglhppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypy
dsrpenvlafenaeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakp
dfmgvnyyqtttvehnppdgvgegvmnttgkkgtstssgipglfktvrnphvdttnwdwa
idpvglriglrrianryqlpilitenglgefdtlepgdivnddyridylrrhvqeiqrai
tdgvdvlgycawsftdllswlngyqkrygfvyvnrddesekdlrrikkksfywyqrviet
ngael

    Sequence, based on observed residues (ATOM records): (download) 
    >5okeD (D:)
hlkpfppeflwgaasaayqvegawnedgkglsvwdvfakqpgrtfkgtngdvavdhyhry
qedvalmaemglkayrfsvswsrvfpdgngavnekgldfydrlieelrnhgiepivtlyh
wdvpqalmdaygawesrriiddfdryavtlfqrfgdrvkywvtlnqqnifisfgyrlglh
ppgvkdmkrmyeanhianlanakviqsfrhyvpdgkigpsfayspmypydsrpenvlafe
naeefqnhwwmdvyawgmypqaawnylesqgleptvapgdwellqaakpdfmgvnyyqtt
tvehnppdgvgtssgipglfktvrnphvdttnwdwaidpvglriglrrianryqlpilit
englgefdtlepgdivnddyridylrrhvqeiqraitdgvdvlgycawsftdllswlngy
qkrygfvyvnrddesekdlrrikkksfywyqrvietngael