PDB entry 5m3b

View 5m3b on RCSB PDB site
Description: Structure of cobinamide-bound BtuF mutant W66L, the periplasmic vitamin B12 binding protein in E.coli
Class: transport protein
Keywords: BtuF, Cobinamide, periplasmic binding protein, ABC transporter, Transport protein
Deposited on 2016-10-14, released 2017-03-01
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-10-16, with a file datestamp of 2019-10-11.
Experiment type: XRAY
Resolution: 1.5 Å
R-factor: N/A
AEROSPACI score: 0.48 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Vitamin B12-binding protein
    Species: Escherichia coli [TaxId:83333]
    Gene: btuF, yadT, b0158, JW0154
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37028 (Start-268)
      • engineered mutation (68)
    Domains in SCOPe 2.08: d5m3ba_
  • Chain 'B':
    Compound: Vitamin B12-binding protein
    Species: Escherichia coli [TaxId:83333]
    Gene: btuF, yadT, b0158, JW0154
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37028 (24-268)
      • expression tag (23)
      • engineered mutation (68)
    Domains in SCOPe 2.08: d5m3bb1, d5m3bb2
  • Heterogens: CYN, CBY, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5m3bA (A:)
mkktaiaiavalagfatvaqaasmaaprvitlspantelafaagitpvgvssysdyppqa
qkieqvstlqgmnlerivalkpdlviawrggnaerqvdqlaslgikvmwvdatsieqian
alrqlapwspqpdkaeqaaqslldqyaqlkaqyadkpkkrvflqfginppftsgkesiqn
qvlevcggenifkdsrvpwpqvsreqvlarspqaivitggpdqipkikqywgeqlkipvi
pltsdwferaspriilaaqqlcnalsqvdsgslevlfqgpggshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5m3bA (A:)
aprvitlspantelafaagitpvgvssysdyppqaqkieqvstlqgmnlerivalkpdlv
iawrggnaerqvdqlaslgikvmwvdatsieqianalrqlapwspqpdkaeqaaqslldq
yaqlkaqyadkpkkrvflqfginppftsgkesiqnqvlevcggenifkdsrvpwpqvsre
qvlarspqaivitgkipvipltsdwferaspriilaaqqlcnalsqvd

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5m3bB (B:)
mkktaiaiavalagfatvaqaasmaaprvitlspantelafaagitpvgvssysdyppqa
qkieqvstlqgmnlerivalkpdlviawrggnaerqvdqlaslgikvmwvdatsieqian
alrqlapwspqpdkaeqaaqslldqyaqlkaqyadkpkkrvflqfginppftsgkesiqn
qvlevcggenifkdsrvpwpqvsreqvlarspqaivitggpdqipkikqywgeqlkipvi
pltsdwferaspriilaaqqlcnalsqvdsgslevlfqgpggshhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >5m3bB (B:)
maaprvitlspantelafaagitpvgvssysdyppqaqkieqvstlqgmnlerivalkpd
lviawrggnaerqvdqlaslgikvmwvdatsieqianalrqlapwspqpdkaeqaaqsll
dqyaqlkaqyadkpkkrvflqfginppftsgkesiqnqvlevcggenifkdsrvpwpqvs
reqvlarspqaivitgkipvipltsdwferaspriilaaqqlcnalsqvd