PDB entry 5m34

View 5m34 on RCSB PDB site
Description: Structure of cobinamide-bound BtuF mutant W66Y, the periplasmic vitamin B12 binding protein in E.coli
Class: transport protein
Keywords: BtuF, Cobinamide, periplasmic binding protein, ABC transporter, Transport protein
Deposited on 2016-10-14, released 2017-03-01
The last revision prior to the SCOPe 2.06 freeze date was dated 2017-03-01, with a file datestamp of 2017-02-24.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: N/A
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Vitamin B12-binding protein
    Species: Escherichia coli [TaxId:83333]
    Gene: btuF, yadT, b0158, JW0154
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37028 (24-268)
      • engineered mutation (68)
    Domains in SCOPe 2.06: d5m34a_
  • Chain 'B':
    Compound: Vitamin B12-binding protein
    Species: Escherichia coli [TaxId:83333]
    Gene: btuF, yadT, b0158, JW0154
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37028 (24-End)
      • expression tag (23)
      • engineered mutation (68)
    Domains in SCOPe 2.06: d5m34b1, d5m34b2
  • Heterogens: CBY, CYN, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >5m34A (A:)
    mkktaiaiavalagfatvaqaasmaaprvitlspantelafaagitpvgvssysdyppqa
    qkieqvstyqgmnlerivalkpdlviawrggnaerqvdqlaslgikvmwvdatsieqian
    alrqlapwspqpdkaeqaaqslldqyaqlkaqyadkpkkrvflqfginppftsgkesiqn
    qvlevcggenifkdsrvpwpqvsreqvlarspqaivitggpdqipkikqywgeqlkipvi
    pltsdwferaspriilaaqqlcnalsqvdsgslevlfqgpggshhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >5m34A (A:)
    aaprvitlspantelafaagitpvgvssysdyppqaqkieqvstyqgmnlerivalkpdl
    viawrggnaerqvdqlaslgikvmwvdatsieqianalrqlapwspqpdkaeqaaqslld
    qyaqlkaqyadkpkkrvflqfginppftsgkesiqnqvlevcggenifkdsrvpwpqvsr
    eqvlarspqaivitkipvipltsdwferaspriilaaqqlcnalsqvd
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >5m34B (B:)
    mkktaiaiavalagfatvaqaasmaaprvitlspantelafaagitpvgvssysdyppqa
    qkieqvstyqgmnlerivalkpdlviawrggnaerqvdqlaslgikvmwvdatsieqian
    alrqlapwspqpdkaeqaaqslldqyaqlkaqyadkpkkrvflqfginppftsgkesiqn
    qvlevcggenifkdsrvpwpqvsreqvlarspqaivitggpdqipkikqywgeqlkipvi
    pltsdwferaspriilaaqqlcnalsqvdsgslevlfqgpggshhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >5m34B (B:)
    maaprvitlspantelafaagitpvgvssysdyppqaqkieqvstyqgmnlerivalkpd
    lviawrggnaerqvdqlaslgikvmwvdatsieqianalrqlapwspqpdkaeqaaqsll
    dqyaqlkaqyadkpkkrvflqfginppftsgkesiqnqvlevcggenifkdsrvpwpqvs
    reqvlarspqaivitgkipvipltsdwferaspriilaaqqlcnalsqv