PDB entry 5kja

View 5kja on RCSB PDB site
Description: Synechocystis apocarotenoid oxygenase (ACO) mutant - Trp149Ala
Class: oxidoreductase
Keywords: iron-coordination, active site, carotenoid binding, ligand interaction, non-heme iron, mutagenesis, OXIDOREDUCTASE
Deposited on 2016-06-18, released 2016-08-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-12-11, with a file datestamp of 2019-12-06.
Experiment type: XRAY
Resolution: 2.8 Å
R-factor: N/A
AEROSPACI score: 0.16 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (Start-489)
      • engineered mutation (148)
    Domains in SCOPe 2.08: d5kjaa_
  • Chain 'B':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (Start-489)
      • engineered mutation (148)
    Domains in SCOPe 2.08: d5kjab_
  • Chain 'C':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (Start-489)
      • engineered mutation (148)
    Domains in SCOPe 2.08: d5kjac_
  • Chain 'D':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (Start-489)
      • engineered mutation (148)
    Domains in SCOPe 2.08: d5kjad_
  • Chain 'E':
    Compound: Apocarotenoid-15,15'-oxygenase
    Species: Synechocystis sp. (strain PCC 6803 / Kazusa) [TaxId:1111708]
    Gene: sll1541
    Database cross-references and differences (RAF-indexed):
    • Uniprot P74334 (Start-489)
      • engineered mutation (148)
    Domains in SCOPe 2.08: d5kjae_
  • Heterogens: CL, FE2, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5kjaA (A:)
mvtspptsspsqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpgllei
gdrplkhpfdgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpagg
wlktifdlrlkniantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqpl
sahpridpastfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdf
aitphyaiflqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqag
fvfhhanafeengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatve
kqlmvsrccefpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfap
hgfagepifvprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhip
yplhgswaqt

    Sequence, based on observed residues (ATOM records): (download) 
    >5kjaA (A:)
spsqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhp
fdgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdl
rlkniantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqplsahpridp
astfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyai
flqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhana
feengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrc
cefpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepi
fvprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswa
qt

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5kjaB (B:)
mvtspptsspsqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpgllei
gdrplkhpfdgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpagg
wlktifdlrlkniantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqpl
sahpridpastfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdf
aitphyaiflqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqag
fvfhhanafeengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatve
kqlmvsrccefpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfap
hgfagepifvprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhip
yplhgswaqt

    Sequence, based on observed residues (ATOM records): (download) 
    >5kjaB (B:)
qrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpfdg
dgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlrlk
niantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqplsahpridpast
fdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaiflq
nnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanafee
ngkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccef
pvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvp
rpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5kjaC (C:)
mvtspptsspsqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpgllei
gdrplkhpfdgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpagg
wlktifdlrlkniantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqpl
sahpridpastfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdf
aitphyaiflqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqag
fvfhhanafeengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatve
kqlmvsrccefpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfap
hgfagepifvprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhip
yplhgswaqt

    Sequence, based on observed residues (ATOM records): (download) 
    >5kjaC (C:)
qrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpfdg
dgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlrlk
niantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqplsahpridpast
fdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaiflq
nnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanafee
ngkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccef
pvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvp
rpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5kjaD (D:)
mvtspptsspsqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpgllei
gdrplkhpfdgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpagg
wlktifdlrlkniantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqpl
sahpridpastfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdf
aitphyaiflqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqag
fvfhhanafeengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatve
kqlmvsrccefpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfap
hgfagepifvprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhip
yplhgswaqt

    Sequence, based on observed residues (ATOM records): (download) 
    >5kjaD (D:)
qrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpfdg
dgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlrlk
niantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqplsahpridpast
fdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaiflq
nnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanafee
ngkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccef
pvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvp
rpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >5kjaE (E:)
mvtspptsspsqrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpgllei
gdrplkhpfdgdgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpagg
wlktifdlrlkniantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqpl
sahpridpastfdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdf
aitphyaiflqnnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqag
fvfhhanafeengkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatve
kqlmvsrccefpvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfap
hgfagepifvprpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhip
yplhgswaqt

    Sequence, based on observed residues (ATOM records): (download) 
    >5kjaE (E:)
qrsyspqdwlrgyqsqpqewdywvedvegsippdlqgtlyrngpglleigdrplkhpfdg
dgmvtafkfpgdgrvhfqskfvrtqgyveeqkagkmiyrgvfgsqpaggwlktifdlrlk
niantnitywgdrllalaeggqphrlepsnlatiglddlggilaegqplsahpridpast
fdggqpcyvtfsiksslsstltlleldpqgkllrqktetfpgfafihdfaitphyaiflq
nnvtlnglpylfglrgagecvqfhpdkpaqiilvprdggeikripvqagfvfhhanafee
ngkiildsicynslpqvdtdgdfrstnfdnldpgqlwrftidpaaatvekqlmvsrccef
pvvhpqqvgrpyryvymgaahhstgnaplqailkvdlesgtetlrsfaphgfagepifvp
rpggvaeddgwllcliykadlhrselvildaqditapaiatlklkhhipyplhgswaqt