SCOPe 2.07: Structural Classification of Proteins

PDB entry 5kaf

View 5kaf on RCSB PDB site
Description: RT XFEL structure of Photosystem II in the dark state at 3.0 A resolution
Class: electron transport
Keywords: photosystems, transmembrane, room temperature, electron transport
Deposited on 2016-06-01, released 2016-11-23
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-09-27, with a file datestamp of 2017-09-22.
Experiment type: XRAY
Resolution: 3 Å
R-factor: N/A
AEROSPACI score: 0.14 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: Photosystem II protein D1 1
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P0A444 (Start-343)
Domains in SCOPe 2.07: d5kafa_
Chain 'B':
Compound: Photosystem II CP47 reaction center protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIQ1
Domains in SCOPe 2.07: d5kafb_
Chain 'C':
Compound: Photosystem II CP43 reaction center protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIF8 (Start-460)
Domains in SCOPe 2.07: d5kafc_
Chain 'D':
Compound: Photosystem II D2 protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8CM25 (Start-351)
Domains in SCOPe 2.07: d5kafd_
Chain 'E':
Compound: Cytochrome b559 subunit alpha
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIP0 (Start-83)
Domains in SCOPe 2.07: d5kafe_
Chain 'F':
Compound: Cytochrome b559 subunit beta
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIN9 (Start-44)
Chain 'H':
Compound: Photosystem II reaction center protein H
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DJ43
Domains in SCOPe 2.07: d5kafh_
Chain 'I':
Compound: Photosystem II reaction center protein I
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DJZ6 (1-End)
  - expression tag (0)
Domains in SCOPe 2.07: d5kafi1, d5kafi2
Chain 'J':
Compound: Photosystem II reaction center protein J
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P59087 (Start-39)
Domains in SCOPe 2.07: d5kafj_
Chain 'K':
Compound: Photosystem II reaction center protein K
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q9F1K9 (Start-45)
Chain 'L':
Compound: Photosystem II reaction center protein L
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIN8 (0-36)
Chain 'M':
Compound: Photosystem II reaction center protein M
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DHA7 (1-End)
  - expression tag (0)
Domains in SCOPe 2.07: d5kafm1, d5kafm2
Chain 'O':
Compound: Photosystem II manganese-stabilizing polypeptide
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P0A431 (Start-271)
Chain 'R':
Compound: Photosystem II protein Y
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DKM3
Chain 'T':
Compound: Photosystem II reaction center protein T
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIQ0 (1-End)
  - expression tag (0)
Domains in SCOPe 2.07: d5kaft1, d5kaft2
Chain 'U':
Compound: Photosystem II 12 kDa extrinsic protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q9F1L5 (Start-133)
Domains in SCOPe 2.07: d5kafu_
Chain 'V':
Compound: cytochrome c-550
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P0A386 (Start-162)
Chain 'X':
Compound: Photosystem II reaction center X protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q9F1R6
Domains in SCOPe 2.07: d5kafx_
Chain 'Y':
Compound: Photosystem II reaction center protein ycf12
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DJI1 (Start-45)
Chain 'Z':
Compound: Photosystem II reaction center protein Z
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DHJ2 (0-61)
Domains in SCOPe 2.07: d5kafz_
Chain 'a':
Compound: Photosystem II protein D1 1
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P0A444 (Start-343)
Chain 'b':
Compound: Photosystem II CP47 reaction center protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIQ1
Chain 'c':
Compound: Photosystem II CP43 reaction center protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIF8 (Start-460)
Chain 'd':
Compound: Photosystem II D2 protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8CM25 (Start-351)
Chain 'e':
Compound: Cytochrome b559 subunit alpha
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIP0 (Start-83)
Chain 'f':
Compound: Cytochrome b559 subunit beta
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIN9 (Start-44)
Domains in SCOPe 2.07: d5kaff_
Chain 'h':
Compound: Photosystem II reaction center protein H
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DJ43
Chain 'i':
Compound: Photosystem II reaction center protein I
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DJZ6 (1-End)
  - expression tag (0)
Chain 'j':
Compound: Photosystem II reaction center protein J
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P59087 (Start-39)
Chain 'k':
Compound: Photosystem II reaction center protein K
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q9F1K9 (Start-45)
Domains in SCOPe 2.07: d5kafk_
Chain 'l':
Compound: Photosystem II reaction center protein L
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIN8 (0-36)
Domains in SCOPe 2.07: d5kafl_
Chain 'm':
Compound: Photosystem II reaction center protein M
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DHA7 (1-End)
  - expression tag (0)
Chain 'o':
Compound: Photosystem II manganese-stabilizing polypeptide
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P0A431 (Start-271)
Domains in SCOPe 2.07: d5kafo_
Chain 'r':
Compound: Photosystem II protein Y
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DKM3
Chain 't':
Compound: Photosystem II reaction center protein T
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DIQ0 (1-End)
  - expression tag (0)
Chain 'u':
Compound: Photosystem II 12 kDa extrinsic protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q9F1L5 (Start-133)
Chain 'v':
Compound: cytochrome c-550
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot P0A386 (Start-162)
Domains in SCOPe 2.07: d5kafv_
Chain 'x':
Compound: Photosystem II reaction center X protein
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q9F1R6
Chain 'y':
Compound: Photosystem II reaction center protein ycf12
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DJI1 (Start-45)
Chain 'z':
Compound: Photosystem II reaction center protein Z
Species: Thermosynechococcus elongatus (strain BP-1) [TaxId:197221]
Database cross-references and differences (RAF-indexed):
- Uniprot Q8DHJ2 (0-61)
Heterogens: OEX, FE2, SQD, CL, CLA, PHO, BCR, PL9, LMG, UNL, LHG, BCT, DGD, HEM, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>5kafA (A:)
mtttlqrresanlwerfcnwvtstdnrlyvgwfgvimiptllaaticfviafiaappvdi
dgirepvsgsllygnniitgavvpssnaiglhfypiweaasldewlynggpyqliifhfl
lgascymgrqwelsyrlgmrpwicvaysaplasafavfliypigqgsfsdgmplgisgtf
nfmivfqaehnilmhpfhqlgvagvfggalfcamhgslvtsslirettetesanygykfg
qeeetynivaahgyfgrlifqyasfnnsrslhfflaawpvvgvwftalgistmafnlngf
nfnhsvidakgnvintwadiinranlgmevmhernahnfpldla

Sequence, based on observed residues (ATOM records): (download)

>5kafA (A:)
anlwerfcnwvtstdnrlyvgwfgvimiptllaaticfviafiaappvdidgirepvsgs
llygnniitgavvpssnaiglhfypiweaasldewlynggpyqliifhfllgascymgrq
welsyrlgmrpwicvaysaplasafavfliypigqgsfsdgmplgisgtfnfmivfqaeh
nilmhpfhqlgvagvfggalfcamhgslvtsslirettetesanygykfgqeeetyniva
ahgyfgrlifqyasfnnsrslhfflaawpvvgvwftalgistmafnlngfnfnhsvidak
gnvintwadiinranlgmevmhernahnfpldla

Chain 'B':
Sequence, based on SEQRES records: (download)

>5kafB (B:)
mglpwyrvhtvlindpgrliaahlmhtalvagwagsmalyelatfdpsdpvlnpmwrqgm
fvlpfmarlgvtgswsgwsitgetgidpgfwsfegvalahivlsgllflaacwhwvywdl
elfrdprtgepaldlpkmfgihlflagllcfgfgafhltglfgpgmwvsdpygltgsvqp
vapewgpdgfnpynpggvvahhiaagivgiiaglfhilvrppqrlykalrmgnietvlss
siaavffaafvvagtmwygsattpielfgptryqwdssyfqqeinrrvqaslasgatlee
awsaipeklafydyignnpakgglfrtgpmnkgdgiaqawkghavfrnkegeelfvrrmp
affesfpviltdkngvvkadipfrraeskysfeqqgvtvsfyggelngqtftdpptvksy
arkaifgeifefdtetlnsdgifrtsprgwftfahavfallfffghiwhgartlfrdvfs
gidpelspeqvewgfyqkvgdvttrrkeav

Sequence, based on observed residues (ATOM records): (download)

>5kafB (B:)
glpwyrvhtvlindpgrliaahlmhtalvagwagsmalyelatfdpsdpvlnpmwrqgmf
vlpfmarlgvtgswsgwsitgetgidpgfwsfegvalahivlsgllflaacwhwvywdle
lfrdprtgepaldlpkmfgihlflagllcfgfgafhltglfgpgmwvsdpygltgsvqpv
apewgpdgfnpynpggvvahhiaagivgiiaglfhilvrppqrlykalrmgnietvlsss
iaavffaafvvagtmwygsattpielfgptryqwdssyfqqeinrrvqaslasgatleea
wsaipeklafydyignnpakgglfrtgpmnkgdgiaqawkghavfrnkegeelfvrrmpa
ffesfpviltdkngvvkadipfrraeskysfeqqgvtvsfyggelngqtftdpptvksya
rkaifgeifefdtetlnsdgifrtsprgwftfahavfallfffghiwhgartlfrdvfsg
idpelspeqvewgfyqkvgdvttr

Chain 'C':
Sequence, based on SEQRES records: (download)

>5kafC (C:)
mvtlssnsifatnrdqessgfawwagnarlinlsgkllgahvahaglivfwagamtlfel
ahfipekpmyeqgliliphiatlgwgvgpggevvdtfpffvvgvvhlissavlgfggvyh
airgpetleeyssffgydwkdknkmttilgfhlivlgigalllvakamffgglydtwapg
ggdvrvitnptldprvifgyllkspfggegwivsvnnledvvgghiwigliciaggiwhi
lttpfgwarrafiwsgeaylsyslgalsmmgfiatcfvwfnntvypsefygptgpeasqa
qamtflirdqklganvgsaqgptglgkylmrsptgeiifggetmrfwdfrgpwleplrgp
ngldlnkikndiqpwqerraaeymthaplgslnsvggvateinsvnfvsprswlatshfv
laffflvghlwhagraraaaagfekgidresepvlsmpsld

Sequence, based on observed residues (ATOM records): (download)

>5kafC (C:)
atnrdqessgfawwagnarlinlsgkllgahvahaglivfwagamtlfelahfipekpmy
eqgliliphiatlgwgvgpggevvdtfpffvvgvvhlissavlgfggvyhairgpetlee
yssffgydwkdknkmttilgfhlivlgigalllvakamffgglydtwapgggdvrvitnp
tldprvifgyllkspfggegwivsvnnledvvgghiwigliciaggiwhilttpfgwarr
afiwsgeaylsyslgalsmmgfiatcfvwfnntvypsefygptgpeasqaqamtflirdq
klganvgsaqgptglgkylmrsptgeiifggetmrfwdfrgpwleplrgpngldlnkikn
diqpwqerraaeymthaplgslnsvggvateinsvnfvsprswlatshfvlaffflvghl
whagraraaaagfekgidresepvlsmpsld

Chain 'D':
Sequence, based on SEQRES records: (download)

>5kafD (D:)
mtiaigrapaergwfdilddwlkrdrfvfvgwsgillfpcaylalggwltgttfvtswyt
hglassylegcnfltvavstpansmghsllllwgpeaqgdftrwcqlgglwtfialhgaf
gligfmlrqfeiarlvgvrpynaiafsapiavfvsvfliyplgqsswffapsfgvaaifr
fllffqgfhnwtlnpfhmmgvagvlggallcaihgatventlfqdgegastfrafnptqa
eetysmvtanrfwsqifgiafsnkrwlhffmlfvpvtglwmsaigvvglalnlrsydfis
qeiraaedpefetfytknlllnegirawmapqdqphenfvfpeevlprgnal

Sequence, based on observed residues (ATOM records): (download)

>5kafD (D:)
rgwfdilddwlkrdrfvfvgwsgillfpcaylalggwltgttfvtswythglassylegc
nfltvavstpansmghsllllwgpeaqgdftrwcqlgglwtfialhgafgligfmlrqfe
iarlvgvrpynaiafsapiavfvsvfliyplgqsswffapsfgvaaifrfllffqgfhnw
tlnpfhmmgvagvlggallcaihgatventlfqdgegastfrafnptqaeetysmvtanr
fwsqifgiafsnkrwlhffmlfvpvtglwmsaigvvglalnlrsydfisqeiraaedpef
etfytknlllnegirawmapqdqphenfvfpeevlprgnal

Chain 'E':
Sequence, based on SEQRES records: (download)

>5kafE (E:)
magttgerpfsdiitsvrywvihsitipalfiagwlfvstglaydvfgtprpdsyyaqeq
rsiplvtdrfeakqqvetfleqlk

Sequence, based on observed residues (ATOM records): (download)

>5kafE (E:)
ttgerpfsdiitsvrywvihsitipalfiagwlfvstglaydvfgtprpdsyyaqeqrsi
plvtdrfeakqqvetfleqlk

Chain 'F':
No sequence available.

Chain 'H':
Sequence, based on SEQRES records: (download)

>5kafH (H:)
marrtwlgdilrplnseygkvapgwgttplmavfmglflvflliileiynstlildgvnv
swkalg

Sequence, based on observed residues (ATOM records): (download)

>5kafH (H:)
arrtwlgdilrplnseygkvapgwgttplmavfmglflvflliileiynstlildgvnvs
wka

Chain 'I':
Sequence, based on SEQRES records: (download)
```
>5kafI (I:)
metlkitvyivvtffvllfvfgflsgdparnpkrkdle
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kafI (I:)
metlkitvyivvtffvllfvfgflsgdparnpkrkd
```
Chain 'J':
Sequence, based on SEQRES records: (download)
```
>5kafJ (J:)
mmseggriplwivatvagmgvivivglffygayaglgssl
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kafJ (J:)
ggriplwivatvagmgvivivglffygayaglgssl
```
Chain 'K':
No sequence available.
Chain 'L':
No sequence available.
Chain 'M':
Sequence, based on SEQRES records: (download)
```
>5kafM (M:)
mevnqlgliatalfvlvpsvfliilyvqtesqqkss
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kafM (M:)
mevnqlgliatalfvlvpsvfliilyvqtesqq
```
Chain 'O':
No sequence available.
Chain 'R':
No sequence available.
Chain 'T':
Sequence, based on SEQRES records: (download)
```
>5kafT (T:)
metityvfifaciialfffaiffreppritkk
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kafT (T:)
metityvfifaciialfffaiffrepprit
```

Chain 'U':
Sequence, based on SEQRES records: (download)

>5kafU (U:)
mqrlgrwlalayfvgvsllgwinwsaptlaatasteeelvnvvdeklgtaygekidlnnt
niaafiqyrglyptlaklivknapyesvedvlnipglterqkqilrenlehftvteveta
lveggdrynnglyk

Sequence, based on observed residues (ATOM records): (download)

>5kafU (U:)
elvnvvdeklgtaygekidlnntniaafiqyrglyptlaklivknapyesvedvlnipgl
terqkqilrenlehftvtevetalveggdrynnglyk

Chain 'V':
No sequence available.
Chain 'X':
Sequence, based on SEQRES records: (download)
```
>5kafX (X:)
mtitpslkgffigllsgavvlgltfavliaisqidkvqrsl
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kafX (X:)
titpslkgffigllsgavvlgltfavliaisqidkvqr
```
Chain 'Y':
No sequence available.

Chain 'Z':
Sequence; same for both SEQRES and ATOM records: (download)

>5kafZ (Z:)
mtilfqlalaalvilsfvmvigvpvayaspqdwdrskqliflgsglwialvlvvgvlnff
vv

Chain 'a':
No sequence available.
Chain 'b':
No sequence available.
Chain 'c':
No sequence available.
Chain 'd':
No sequence available.
Chain 'e':
No sequence available.
Chain 'f':
Sequence, based on SEQRES records: (download)
```
>5kaff (f:)
mtsntpnqepvsypiftvrwvavhtlavptifflgaiaamqfiqr
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kaff (f:)
sypiftvrwvavhtlavptifflgaiaamqfiqr
```
Chain 'h':
No sequence available.
Chain 'i':
No sequence available.
Chain 'j':
No sequence available.
Chain 'k':
Sequence, based on SEQRES records: (download)
```
>5kafk (k:)
midalvlvaklpeayaifdplvdvlpvipvlflalafvwqaavgfr
```
Sequence, based on observed residues (ATOM records): (download)
```
>5kafk (k:)
klpeayaifdplvdvlpvipvlflalafvwqaavgfr
```
Chain 'l':
Sequence; same for both SEQRES and ATOM records: (download)
```
>5kafl (l:)
mepnpnrqpvelnrtslylglllilvlallfssyffn
```
Chain 'm':
No sequence available.

Chain 'o':
Sequence, based on SEQRES records: (download)

>5kafo (o:)
mkyrilmatllavclgifslsapafaakqtltyddivgtglankcptlddtargaypids
sqtyriarlclqpttflvkeepknkrqeaefvptklvtrettsldqiqgelkvnsdgslt
fveedgidfqpvtvqmaggeripllftvknlvastqpnvtsittstdfkgefnvpsyrta
nfldpkgrglasgydsaialpqakeeelaranvkrfsltkgqislnvakvdgrtgeiagt
feseqlsdddmgahephevkiqgvfyasiepa

Sequence, based on observed residues (ATOM records): (download)

>5kafo (o:)
qtltyddivgtglankcptlddtargaypidssqtyriarlclqpttflvkeepknkrqe
aefvptklvtrettsldqiqgelkvnsdgsltfveedgidfqpvtvqmaggeripllftv
knlvastqpnvtsittstdfkgefnvpsyrtanfldpkgrglasgydsaialpqakeeel
aranvkrfsltkgqislnvakvdgrtgeiagtfeseqlsdddmgahephevkiqgvfyas
iepa

Chain 'r':
No sequence available.
Chain 't':
No sequence available.
Chain 'u':
No sequence available.

Chain 'v':
Sequence, based on SEQRES records: (download)

>5kafv (v:)
mlkkcvwlavalclclwqftmgtalaaeltpevltvplnsegktitltekqylegkrlfq
yacaschvggitktnpsldlrtetlalatpprdnieglvdymknpttydgeqeiaevhps
lrsadifpkmrnltekdlvaiaghilvepkilgdkwgggkvyy

Sequence, based on observed residues (ATOM records): (download)

>5kafv (v:)
aeltpevltvplnsegktitltekqylegkrlfqyacaschvggitktnpsldlrtetla
latpprdnieglvdymknpttydgeqeiaevhpslrsadifpkmrnltekdlvaiaghil
vepkilgdkwgggkvyy

Chain 'x':
No sequence available.
Chain 'y':
No sequence available.
Chain 'z':
No sequence available.