PDB entry 5hq3

View 5hq3 on RCSB PDB site
Description: Stable, high-expression variant of human acetylcholinesterase
Class: hydrolase
Keywords: Design, De Novo Protein, hydrolase
Deposited on 2016-01-21, released 2016-07-27
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-11-08, with a file datestamp of 2017-11-03.
Experiment type: XRAY
Resolution: 2.6 Å
R-factor: N/A
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: acetylcholinesterase
    Species: Homo sapiens [TaxId:9606]
    Gene: ACHE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22303
      • engineered mutation (12)
      • engineered mutation (23)
      • engineered mutation (42)
      • engineered mutation (48)
      • engineered mutation (60)
      • engineered mutation (67)
      • engineered mutation (91)
      • engineered mutation (109-110)
      • engineered mutation (112)
      • engineered mutation (115)
      • engineered mutation (127)
      • engineered mutation (140-141)
      • engineered mutation (144)
      • engineered mutation (187)
      • engineered mutation (226)
      • engineered mutation (234)
      • engineered mutation (238)
      • engineered mutation (240-242)
      • engineered mutation (249)
      • engineered mutation (253)
      • engineered mutation (275)
      • engineered mutation (278)
      • engineered mutation (280)
      • engineered mutation (309)
      • engineered mutation (318)
      • engineered mutation (322)
      • engineered mutation (325)
      • engineered mutation (331)
      • engineered mutation (357)
      • engineered mutation (361)
      • engineered mutation (378)
      • engineered mutation (393-394)
      • engineered mutation (396)
      • engineered mutation (408)
      • engineered mutation (414)
      • engineered mutation (416)
      • engineered mutation (418)
      • engineered mutation (421)
      • engineered mutation (434)
      • engineered mutation (438)
      • engineered mutation (441)
      • engineered mutation (467)
      • engineered mutation (474)
      • engineered mutation (506-507)
      • engineered mutation (509)
    Domains in SCOPe 2.07: d5hq3a_
  • Chain 'B':
    Compound: acetylcholinesterase
    Species: Homo sapiens [TaxId:9606]
    Gene: ACHE
    Database cross-references and differences (RAF-indexed):
    • Uniprot P22303
      • engineered mutation (12)
      • engineered mutation (23)
      • engineered mutation (42)
      • engineered mutation (48)
      • engineered mutation (60)
      • engineered mutation (67)
      • engineered mutation (91)
      • engineered mutation (109-110)
      • engineered mutation (112)
      • engineered mutation (115)
      • engineered mutation (127)
      • engineered mutation (140-141)
      • engineered mutation (144)
      • engineered mutation (187)
      • engineered mutation (226)
      • engineered mutation (234)
      • engineered mutation (238)
      • engineered mutation (240-242)
      • engineered mutation (249)
      • engineered mutation (253)
      • engineered mutation (275)
      • engineered mutation (278)
      • engineered mutation (280)
      • engineered mutation (309)
      • engineered mutation (318)
      • engineered mutation (322)
      • engineered mutation (325)
      • engineered mutation (331)
      • engineered mutation (357)
      • engineered mutation (361)
      • engineered mutation (378)
      • engineered mutation (393-394)
      • engineered mutation (396)
      • engineered mutation (408)
      • engineered mutation (414)
      • engineered mutation (416)
      • engineered mutation (418)
      • engineered mutation (421)
      • engineered mutation (434)
      • engineered mutation (438)
      • engineered mutation (441)
      • engineered mutation (467)
      • engineered mutation (474)
      • engineered mutation (506-507)
      • engineered mutation (509)
    Domains in SCOPe 2.07: d5hq3b_
  • Heterogens: VX, MES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5hq3A (A:)
megredaellvttrggrlrgirlttpggpvsaflgipfaeppvgprrfrppepkqpwsgv
wdattfqnvcyqyvdtlypgfegtemwnpnrnlsedclylnvwtpyprpknpapvmvwiy
gggfysgsssldvydgrflvrtervvlvsmnyrvgafgflalpgsreapgnvglldqrla
lqwvqeniaafggdptsvtlfgesagaasvgmhllsppsrglfhrailqsgapnapwayv
sreearrralqlaklvgcppggtggndtelvaclrnrppqelvnhewhvlpqesvfrfsf
vpvvdgdflpdtpealinngdfkgldvlvgvnkdegsyflvygapgfskdneslisreef
legvrvgvpqvsdlaaeaivlhytdwlhpedpaknrdalsdvvgdhnvicpvaqfaqrya
angarvyayvfehrsstlpwpewmgvphgyeiefifgipldpsrnytkeekifarrlmry
wanfartgdpneprdpkapqwppytadeqkyvsldlrplevrrglraqacafwnrflpkl
lsatdtld

    Sequence, based on observed residues (ATOM records): (download) 
    >5hq3A (A:)
daellvttrggrlrgirlttpggpvsaflgipfaeppvgprrfrppepkqpwsgvwdatt
fqnvcyqyvdtlypgfegtemwnpnrnlsedclylnvwtpyprpknpapvmvwiygggfy
sgsssldvydgrflvrtervvlvsmnyrvgafgflalpgsreapgnvglldqrlalqwvq
eniaafggdptsvtlfgesagaasvgmhllsppsrglfhrailqsgapnapwayvsreea
rrralqlaklvgcppndtelvaclrnrppqelvnhewhvlpqesvfrfsfvpvvdgdflp
dtpealinngdfkgldvlvgvnkdegsyflvygapgfskdneslisreeflegvrvgvpq
vsdlaaeaivlhytdwlhpedpaknrdalsdvvgdhnvicpvaqfaqryaangarvyayv
fehrsstlpwpewmgvphgyeiefifgipldpsrnytkeekifarrlmrywanfartgdp
neprpqwppytadeqkyvsldlrplevrrglraqacafwnrflpkllsa

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5hq3B (B:)
megredaellvttrggrlrgirlttpggpvsaflgipfaeppvgprrfrppepkqpwsgv
wdattfqnvcyqyvdtlypgfegtemwnpnrnlsedclylnvwtpyprpknpapvmvwiy
gggfysgsssldvydgrflvrtervvlvsmnyrvgafgflalpgsreapgnvglldqrla
lqwvqeniaafggdptsvtlfgesagaasvgmhllsppsrglfhrailqsgapnapwayv
sreearrralqlaklvgcppggtggndtelvaclrnrppqelvnhewhvlpqesvfrfsf
vpvvdgdflpdtpealinngdfkgldvlvgvnkdegsyflvygapgfskdneslisreef
legvrvgvpqvsdlaaeaivlhytdwlhpedpaknrdalsdvvgdhnvicpvaqfaqrya
angarvyayvfehrsstlpwpewmgvphgyeiefifgipldpsrnytkeekifarrlmry
wanfartgdpneprdpkapqwppytadeqkyvsldlrplevrrglraqacafwnrflpkl
lsatdtlde

    Sequence, based on observed residues (ATOM records): (download) 
    >5hq3B (B:)
aellvttrggrlrgirlttpggpvsaflgipfaeppvgprrfrppepkqpwsgvwdattf
qnvcyqyvdtlypgfegtemwnpnrnlsedclylnvwtpyprpknpapvmvwiygggfys
gsssldvydgrflvrtervvlvsmnyrvgafgflalpgsreapgnvglldqrlalqwvqe
niaafggdptsvtlfgesagaasvgmhllsppsrglfhrailqsgapnapwayvsreear
rralqlaklvgcppndtelvaclrnrppqelvnhewhvlpqesvfrfsfvpvvdgdflpd
tpealinngdfkgldvlvgvnkdegsyflvygapgfskdneslisreeflegvrvgvpqv
sdlaaeaivlhytdwlhpedpaknrdalsdvvgdhnvicpvaqfaqryaangarvyayvf
ehrsstlpwpewmgvphgyeiefifgipldpsrnytkeekifarrlmrywanfartgdpn
eppqwppytadeqkyvsldlrplevrrglraqacafwnrflpkllsat