PDB entry 5gwt

View 5gwt on RCSB PDB site
Description: 4-hydroxyisoleucine dehydrogenase mutant complexed with NADH and succinate
Class: oxidoreductase
Keywords: NADH-dependent, short-chain, dehydrogenase, reductase, OXIDOREDUCTASE
Deposited on 2016-09-13, released 2017-10-25
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-11-08, with a file datestamp of 2017-11-03.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: N/A
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: 4-hydroxyisolecuine dehydrogenase
    Species: Bacillus thuringiensis [TaxId:1428]
    Gene: AC241_05390
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A0K0Q8K4 (0-End)
      • engineered mutation (139)
      • engineered mutation (237)
    Domains in SCOPe 2.07: d5gwta_
  • Chain 'B':
    Compound: 4-hydroxyisolecuine dehydrogenase
    Species: Bacillus thuringiensis [TaxId:1428]
    Gene: AC241_05390
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A0K0Q8K4 (0-End)
      • engineered mutation (139)
      • engineered mutation (237)
    Domains in SCOPe 2.07: d5gwtb_
  • Chain 'C':
    Compound: 4-hydroxyisolecuine dehydrogenase
    Species: Bacillus thuringiensis [TaxId:1428]
    Gene: AC241_05390
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A0K0Q8K4 (0-End)
      • engineered mutation (139)
      • engineered mutation (237)
    Domains in SCOPe 2.07: d5gwtc_
  • Chain 'D':
    Compound: 4-hydroxyisolecuine dehydrogenase
    Species: Bacillus thuringiensis [TaxId:1428]
    Gene: AC241_05390
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A0K0Q8K4 (0-End)
      • engineered mutation (139)
      • engineered mutation (237)
    Domains in SCOPe 2.07: d5gwtd_
  • Heterogens: NAD, SIN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >5gwtA (A:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gknllekgelnsklegkpipnpllgldstrtghhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >5gwtA (A:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gkn
    

  • Chain 'B':
    Sequence, based on SEQRES records: (download)
    >5gwtB (B:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gknllekgelnsklegkpipnpllgldstrtghhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >5gwtB (B:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gkn
    

  • Chain 'C':
    Sequence, based on SEQRES records: (download)
    >5gwtC (C:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gknllekgelnsklegkpipnpllgldstrtghhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >5gwtC (C:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gkn
    

  • Chain 'D':
    Sequence, based on SEQRES records: (download)
    >5gwtD (D:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gknllekgelnsklegkpipnpllgldstrtghhhhhh
    

    Sequence, based on observed residues (ATOM records): (download)
    >5gwtD (D:)
    kiimisgansgighacikyfleksfhvialdinnnnlidymktdmplkvvqidlsnseai
    hnlftqldleklspdilinaagireitpvlhlsddmfkkvidvnlvapfilsrevakrwc
    eskikgcivniasvsglmakperaayvaskhaligltkqmamefgkqnirvnsispgvir
    telteeyfsnkalmsmiksnqsldtwglpqdivscieylisdqarfitgsnfvidggqta
    gkn