PDB entry 5gqd

View 5gqd on RCSB PDB site
Description: Crystal structure of covalent glycosyl-enzyme intermediate of xylanase mutant (T82A, N127S, and E128H) from Streptomyces olivaceoviridis E-86
Class: hydrolase
Keywords: xylanase, mutant, pNP-xylobioside, covalent glycosyl-enzyme intermediate, HYDROLASE
Deposited on 2016-08-07, released 2017-08-09
The last revision prior to the SCOPe 2.06 freeze date was dated 2017-08-09, with a file datestamp of 2017-08-04.
Experiment type: XRAY
Resolution: 1.8 Å
R-factor: N/A
AEROSPACI score: 0.37 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Beta-xylanase
    Species: Streptomyces olivaceoviridis [TaxId:1921]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7SI98
      • engineered mutation (81)
      • engineered mutation (126-127)
    Domains in SCOPe 2.06: d5gqda1, d5gqda2
  • Chain 'B':
    Compound: Beta-xylanase
    Species: Streptomyces olivaceoviridis [TaxId:1921]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q7SI98 (0-435)
      • engineered mutation (81)
      • engineered mutation (126-127)
    Domains in SCOPe 2.06: d5gqdb1, d5gqdb2
  • Heterogens: XYP, XYS, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5gqdA (A:)
aestlgaaaaqsgryfgtaiasgklgdsayttiasrefnmvtaenemkidatepqrgqfn
fsagdrvynwavqngkqvrghalawhsqqpgwmqslsgstlrqamidhingvmghykgki
aqwdvvshafsddgsggrrdsnlqrtgndwievafrtaraadpaaklcyndynienwtwa
ktqgvynmvrdfkqrgvpidcvgfqshfnsgspynsnfrttlqnfaalgvdvaiteldiq
gassstyaavtndclavsrclgitvwgvrdtdswrsgdtpllfngdgskkaaytavlnal
nggsstpppsgggqikgvgsgrcldvpnasttdgtqvqlydchsatnqqwtytdagelrv
ygdkcldaagtgngtkvqiyscwggdnqkwrlnsdgsivgvqsglcldavgggtangtli
qlyscsngsnqrwtrt

    Sequence, based on observed residues (ATOM records): (download) 
    >5gqdA (A:)
aestlgaaaaqsgryfgtaiasgklgdsayttiasrefnmvtaenemkidatepqrgqfn
fsagdrvynwavqngkqvrghalawhsqqpgwmqslsgstlrqamidhingvmghykgki
aqwdvvshafsddgsggrrdsnlqrtgndwievafrtaraadpaaklcyndynienwtwa
ktqgvynmvrdfkqrgvpidcvgfqshfnsgspynsnfrttlqnfaalgvdvaiteldiq
gassstyaavtndclavsrclgitvwgvrdtdswrsgdtpllfngdgskkaaytavlnal
ngggqikgvgsgrcldvpnasttdgtqvqlydchsatnqqwtytdagelrvygdkcldaa
gtgngtkvqiyscwggdnqkwrlnsdgsivgvqsglcldavgggtangtliqlyscsngs
nqrwtrt

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5gqdB (B:)
aestlgaaaaqsgryfgtaiasgklgdsayttiasrefnmvtaenemkidatepqrgqfn
fsagdrvynwavqngkqvrghalawhsqqpgwmqslsgstlrqamidhingvmghykgki
aqwdvvshafsddgsggrrdsnlqrtgndwievafrtaraadpaaklcyndynienwtwa
ktqgvynmvrdfkqrgvpidcvgfqshfnsgspynsnfrttlqnfaalgvdvaiteldiq
gassstyaavtndclavsrclgitvwgvrdtdswrsgdtpllfngdgskkaaytavlnal
nggsstpppsgggqikgvgsgrcldvpnasttdgtqvqlydchsatnqqwtytdagelrv
ygdkcldaagtgngtkvqiyscwggdnqkwrlnsdgsivgvqsglcldavgggtangtli
qlyscsngsnqrwtrt

    Sequence, based on observed residues (ATOM records): (download) 
    >5gqdB (B:)
aestlgaaaaqsgryfgtaiasgklgdsayttiasrefnmvtaenemkidatepqrgqfn
fsagdrvynwavqngkqvrghalawhsqqpgwmqslsgstlrqamidhingvmghykgki
aqwdvvshafsddgsggrrdsnlqrtgndwievafrtaraadpaaklcyndynienwtwa
ktqgvynmvrdfkqrgvpidcvgfqshfnsgspynsnfrttlqnfaalgvdvaiteldiq
gassstyaavtndclavsrclgitvwgvrdtdswrsgdtpllfngdgskkaaytavlnal
ngggqikgvgsgrcldvpnasttdgtqvqlydchsatnqqwtytdagelrvygdkcldaa
gtgngtkvqiyscwggdnqkwrlnsdgsivgvqsglcldavgggtangtliqlyscsngs
nqrwtrt