PDB entry 5fbg

View 5fbg on RCSB PDB site
Description: S1 nuclease from Aspergillus oryzae, mutant D65N, in complex with phosphate, 2'-deoxycytidine and 2'-deoxyguanosine.
Class: hydrolase
Keywords: Endonuclease, Zinc dependent, Complex, mutant, hydrolase
Deposited on 2015-12-14, released 2016-12-28
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-06-29.
Experiment type: XRAY
Resolution: 1.97 Å
R-factor: N/A
AEROSPACI score: 0.4 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Nuclease S1
    Species: Aspergillus oryzae RIB40 [TaxId:510516]
    Gene: nucS, AO090001000075
    Database cross-references and differences (RAF-indexed):
    • Uniprot P24021 (0-266)
      • engineered mutation (44)
    Domains in SCOPe 2.08: d5fbga_
  • Chain 'B':
    Compound: Nuclease S1
    Species: Aspergillus oryzae RIB40 [TaxId:510516]
    Gene: nucS, AO090001000075
    Database cross-references and differences (RAF-indexed):
    • Uniprot P24021 (0-End)
      • engineered mutation (44)
    Domains in SCOPe 2.08: d5fbgb_
  • Heterogens: ZN, NAG, PO4, DCZ, GNG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >5fbgA (A:)
wgnlghetvayiaqsfvasstesfcqnilgddstsylanvatwantykytdagefskpyh
fidaqdnppqscgvdydrdcgsagcsisaiqnytnillespngsealnalkfvvhiigdi
hqplhdenleaggngidvtydgettnlhhiwdtnmpeeaaggyslsvaktyadllterik
tgtysskkdswtdgidikdpvstsmiwaadantyvcstvlddglayinstdlsgeyydks
qpvfeeliakagyrlaawldliasqps

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5fbgB (B:)
wgnlghetvayiaqsfvasstesfcqnilgddstsylanvatwantykytdagefskpyh
fidaqdnppqscgvdydrdcgsagcsisaiqnytnillespngsealnalkfvvhiigdi
hqplhdenleaggngidvtydgettnlhhiwdtnmpeeaaggyslsvaktyadllterik
tgtysskkdswtdgidikdpvstsmiwaadantyvcstvlddglayinstdlsgeyydks
qpvfeeliakagyrlaawldliasqps

    Sequence, based on observed residues (ATOM records): (download) 
    >5fbgB (B:)
wgnlghetvayiaqsfvasstesfcqnilgddstsylanvatwantykytdagefskpyh
fidaqdnppqscgvdydrdcgsagcsisaiqnytnillespngsealnalkfvvhiigdi
hqplhdenleaggngidvtydgettnlhhiwdtnmpeeaaggyslsvaktyadllterik
tgtysskkdswtdgidikdpvstsmiwaadantyvcstvlddglayinstdlsgeyydks
qpvfeeliakagyrlaawldliasq