PDB entry 5ekl

View 5ekl on RCSB PDB site
Description: Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature
Class: hydrolase
Keywords: nuclease, hyperstable, pdTp, ionizable group, HYDROLASE
Deposited on 2015-11-03, released 2015-11-18
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-11-18, with a file datestamp of 2015-11-13.
Experiment type: XRAY
Resolution: 2.1 Å
R-factor: N/A
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thermonuclease
    Species: Staphylococcus aureus [TaxId:1280]
    Gene: nuc
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00644
      • engineered mutation (43-44)
      • engineered mutation (55)
      • engineered mutation (93)
      • engineered mutation (110)
      • engineered mutation (117)
      • engineered mutation (121)
    Domains in SCOPe 2.06: d5ekla_
  • Heterogens: CA, THP, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5eklA (A:)
atstkklhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafdkkmv
enakkievefdkgqrtdkygrglayiyadgkmveealvrqglakvayvykgnntheqllr
kaeaqakkeklniwsednadsgq

    Sequence, based on observed residues (ATOM records): (download) 
    >5eklA (A:)
lhkepatlikaidgdtvklmykgqpmtfrlllvdtpefnekygpeasafdkkmvenakki
evefdkgqrtdkygrglayiyadgkmveealvrqglakvayvykgnntheqllrkaeaqa
kkeklniws