PDB entry 5dyz

View 5dyz on RCSB PDB site
Description: Crystal structure of Asp251Gly/Gln307His mutant of cytochrome P450 BM3 in complex with N-palmitoylglycine
Class: oxidoreductase
Keywords: cytochrome P450, random mutagenesis, drug metabolism, substrate, oxidoreductase
Deposited on 2015-09-25, released 2016-01-20
The last revision prior to the SCOPe 2.07 freeze date was dated 2016-06-22, with a file datestamp of 2016-06-17.
Experiment type: XRAY
Resolution: 1.97 Å
R-factor: N/A
AEROSPACI score: 0.33 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Bifunctional P-450/NADPH-P450 reductase
    Species: Bacillus megaterium [TaxId:1404]
    Gene: CYP102A1, cyp102
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14779
      • engineered mutation (250)
      • engineered mutation (306)
    Domains in SCOPe 2.07: d5dyza_
  • Chain 'C':
    Compound: Bifunctional P-450/NADPH-P450 reductase
    Species: Bacillus megaterium [TaxId:1404]
    Gene: CYP102A1, cyp102
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14779
      • engineered mutation (250)
      • engineered mutation (306)
    Domains in SCOPe 2.07: d5dyzc_
  • Heterogens: HEM, 140, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5dyzA (A:)
tikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlike
acdesrfdknlsqalkfvrdfagdglftswtheknwkkahnillpsfsqqamkgyhammv
diavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfitsmvra
ldeamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgeqsddllthmlng
kdpetgepldgeniryqiitfliaghettsgllsfalyflvknphvlqkaaeeaarvlvd
pvpsykhvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdelmvlipql
hrdktiwgddveefrperfenpsaipqhafkpfgngqracigqqfalheatlvlgmmlkh
fdfedhtnyeldiketltlkpegfvvkakskkiplggipspsteqsakkv

    Sequence, based on observed residues (ATOM records): (download) 
    >5dyzA (A:)
ikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlikea
cdesrfdknlsqalkfvrdfagdglftswtheknwkkahnillpsfsqqamkgyhammvd
iavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfitsmvral
deamnklqrenkrqfqedikvmndlvdkiiadrkaseqsddllthmlngkdpetgepldg
eniryqiitfliaghettsgllsfalyflvknphvlqkaaeeaarvlvdpvpsykhvkql
kyvgmvlnealrlwptapafslyakedtvlggeyplekgdelmvlipqlhrdktiwgddv
eefrperfenpsaipqhafkpfgngqracigqqfalheatlvlgmmlkhfdfedhtnyel
diketltlkpegfvvkakskkiplg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5dyzC (C:)
tikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlike
acdesrfdknlsqalkfvrdfagdglftswtheknwkkahnillpsfsqqamkgyhammv
diavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfitsmvra
ldeamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgeqsddllthmlng
kdpetgepldgeniryqiitfliaghettsgllsfalyflvknphvlqkaaeeaarvlvd
pvpsykhvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdelmvlipql
hrdktiwgddveefrperfenpsaipqhafkpfgngqracigqqfalheatlvlgmmlkh
fdfedhtnyeldiketltlkpegfvvkakskkiplggipspsteqsakkv

    Sequence, based on observed residues (ATOM records): (download) 
    >5dyzC (C:)
ikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlikea
cdesrfdknlsqalkfvrdfagdglftswtheknwkkahnillpsfsqqamkgyhammvd
iavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfitsmvral
deamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgqsddllthmlngkd
petgepldgeniryqiitfliaghettsgllsfalyflvknphvlqkaaeeaarvlvdpv
psykhvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdelmvlipqlhr
dktiwgddveefrperfenpsaipqhafkpfgngqracigqqfalheatlvlgmmlkhfd
fedhtnyeldiketltlkpegfvvkakskkipl