PDB entry 5dpj

View 5dpj on RCSB PDB site
Description: sfGFP double mutant - 133/149 p-ethynyl-L-phenylalanine
Class: fluorescent protein
Keywords: GFP, unnatural amino acid, cyanophenylalanine, FLUORESCENT PROTEIN
Deposited on 2015-09-12, released 2016-02-03
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-11-27, with a file datestamp of 2019-11-22.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: N/A
AEROSPACI score: 0.2 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0
      • engineered mutation (30)
      • chromophore (66)
      • engineered mutation (72)
      • engineered mutation (80)
      • engineered mutation (133)
      • engineered mutation (149)
      • engineered mutation (206)
    Domains in SCOPe 2.08: d5dpja_
  • Chain 'B':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0
      • engineered mutation (30)
      • chromophore (66)
      • engineered mutation (72)
      • engineered mutation (80)
      • engineered mutation (133)
      • engineered mutation (149)
      • engineered mutation (206)
    Domains in SCOPe 2.08: d5dpjb_
  • Chain 'C':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0
      • engineered mutation (30)
      • chromophore (66)
      • engineered mutation (72)
      • engineered mutation (80)
      • engineered mutation (133)
      • engineered mutation (149)
      • engineered mutation (206)
    Domains in SCOPe 2.08: d5dpjc_
  • Chain 'D':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A059PIQ0
      • engineered mutation (30)
      • chromophore (66)
      • engineered mutation (72)
      • engineered mutation (80)
      • engineered mutation (133)
      • engineered mutation (149)
      • engineered mutation (206)
    Domains in SCOPe 2.08: d5dpjd_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5dpjA (A:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtl
vnrielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqla
dhyqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >5dpjA (A:)
kgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlvt
tlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvnr
ielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqladhy
qqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagith

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5dpjB (B:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtl
vnrielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqla
dhyqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >5dpjB (B:)
kgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlvt
tlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvnr
ielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqladhy
qqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagi

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5dpjC (C:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtl
vnrielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqla
dhyqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >5dpjC (C:)
kgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlvt
tlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvnr
ielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqladhy
qqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagi

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5dpjD (D:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtl
vnrielkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqla
dhyqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >5dpjD (D:)
eelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwptlvttl
gygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtlvnrie
lkgidfkexgnilghkleynfnshxvyitadkqkngikanfkirhnvedgsvqladhyqq
ntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagith