PDB entry 5b12

View 5b12 on RCSB PDB site
Description: Crystal structure of the B-type halohydrin hydrogen-halide-lyase mutant F71W/Q125T/D199H from Corynebacterium sp. N-1074
Class: lyase
Keywords: Lyase, Enantioselectivity, Halohydrin
Deposited on 2015-11-17, released 2016-08-03
The last revision prior to the SCOPe 2.07 freeze date was dated 2017-02-15, with a file datestamp of 2017-02-10.
Experiment type: XRAY
Resolution: 1.72 Å
R-factor: N/A
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Halohydrin epoxidase B
    Species: Corynebacterium sp. [TaxId:1720]
    Gene: hheB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46347 (Start-226)
      • engineered mutation (70)
      • engineered mutation (124)
      • engineered mutation (198)
    Domains in SCOPe 2.07: d5b12a_
  • Chain 'B':
    Compound: Halohydrin epoxidase B
    Species: Corynebacterium sp. [TaxId:1720]
    Gene: hheB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46347 (2-226)
      • engineered mutation (70)
      • engineered mutation (124)
      • engineered mutation (198)
    Domains in SCOPe 2.07: d5b12b_
  • Chain 'C':
    Compound: Halohydrin epoxidase B
    Species: Corynebacterium sp. [TaxId:1720]
    Gene: hheB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46347 (Start-226)
      • engineered mutation (70)
      • engineered mutation (124)
      • engineered mutation (198)
    Domains in SCOPe 2.07: d5b12c_
  • Chain 'D':
    Compound: Halohydrin epoxidase B
    Species: Corynebacterium sp. [TaxId:1720]
    Gene: hheB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46347 (Start-226)
      • engineered mutation (70)
      • engineered mutation (124)
      • engineered mutation (198)
    Domains in SCOPe 2.07: d5b12d_
  • Chain 'E':
    Compound: Halohydrin epoxidase B
    Species: Corynebacterium sp. [TaxId:1720]
    Gene: hheB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46347 (2-226)
      • engineered mutation (70)
      • engineered mutation (124)
      • engineered mutation (198)
    Domains in SCOPe 2.07: d5b12e_
  • Chain 'F':
    Compound: Halohydrin epoxidase B
    Species: Corynebacterium sp. [TaxId:1720]
    Gene: hheB
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q46347 (Start-226)
      • engineered mutation (70)
      • engineered mutation (124)
      • engineered mutation (198)
    Domains in SCOPe 2.07: d5b12f_
  • Heterogens: CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5b12A (A:)
mkngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidv
lvanfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaa
amrytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdef
kedmarrvplgrlatarehallalflasdesdfivgksiefdggwat

    Sequence, based on observed residues (ATOM records): (download) 
    >5b12A (A:)
grlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidvlva
nfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaaamr
ytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdefked
marrvplgrlatarehallalflasdesdfivgksiefdggwat

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5b12B (B:)
mkngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidv
lvanfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaa
amrytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdef
kedmarrvplgrlatarehallalflasdesdfivgksiefdggwat

    Sequence, based on observed residues (ATOM records): (download) 
    >5b12B (B:)
ngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidvlv
anfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaaam
rytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdefke
dmarrvplgrlatarehallalflasdesdfivgksiefdggwat

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >5b12C (C:)
mkngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidv
lvanfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaa
amrytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdef
kedmarrvplgrlatarehallalflasdesdfivgksiefdggwat

    Sequence, based on observed residues (ATOM records): (download) 
    >5b12C (C:)
grlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidvlva
nfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaaamr
ytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdefked
marrvplgrlatarehallalflasdesdfivgksiefdggwat

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >5b12D (D:)
mkngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidv
lvanfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaa
amrytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdef
kedmarrvplgrlatarehallalflasdesdfivgksiefdggwat

    Sequence, based on observed residues (ATOM records): (download) 
    >5b12D (D:)
grlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidvlva
nfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaaamr
ytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdefked
marrvplgrlatarehallalflasdesdfivgksiefdggwat

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >5b12E (E:)
mkngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidv
lvanfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaa
amrytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdef
kedmarrvplgrlatarehallalflasdesdfivgksiefdggwat

    Sequence, based on observed residues (ATOM records): (download) 
    >5b12E (E:)
ngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidvlv
anfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaaam
rytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdefke
dmarrvplgrlatarehallalflasdesdfivgksiefdggwat

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >5b12F (F:)
mkngrlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidv
lvanfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaa
amrytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdef
kedmarrvplgrlatarehallalflasdesdfivgksiefdggwat

    Sequence, based on observed residues (ATOM records): (download) 
    >5b12F (F:)
grlagkrvlltnadaymgeatvqvfeeegaeviadhtdltkvgaaeevveraghidvlva
nfavdahwgvtvletdeelwqtayetivhplhricravlpqfyernkgkivvygsaaamr
ytegalaystarfaqrgyvtalgpeaarhnvnvnfiaqhwtqnkeyfwperiatdefked
marrvplgrlatarehallalflasdesdfivgksiefdggwat