PDB entry 5aj9

View 5aj9 on RCSB PDB site
Description: G7 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa
Class: hydrolase
Keywords: hydrolase, catalytic promiscuity, directed evolution, neutral drift, sulfatase, superfamily
Deposited on 2015-02-20, released 2016-03-16
The last revision prior to the SCOPe 2.07 freeze date was dated 2016-03-16, with a file datestamp of 2016-03-11.
Experiment type: XRAY
Resolution: 2 Å
R-factor: N/A
AEROSPACI score: 0.31 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot U5R2L4
      • engineered mutation (49-50)
      • engineered mutation (68)
      • engineered mutation (71)
      • engineered mutation (336)
      • engineered mutation (351)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d5aj9a_
  • Chain 'B':
    Compound: arylsulfatase
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot U5R2L4
      • engineered mutation (49-50)
      • engineered mutation (68)
      • engineered mutation (71)
      • engineered mutation (336)
      • engineered mutation (351)
      • engineered mutation (460)
      • engineered mutation (522)
    Domains in SCOPe 2.07: d5aj9b_
  • Heterogens: CL, CA, SO4, MES, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5aj9A (A:)
mskrpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhtasaxsptrsmllt
gtdhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspflvr

    Sequence, based on observed residues (ATOM records): (download) 
    >5aj9A (A:)
krpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhtasaxsptrsmlltgt
dhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkpeq
tphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssd
afgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlar
lkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylr
rqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwygpr
waqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrh
pgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapv
gpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvve

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5aj9B (B:)
mskrpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhtasaxsptrsmllt
gtdhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkp
eqtphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfys
sdafgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerl
arlkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdy
lrrqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwyg
prwaqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagv
rhpgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpa
pvgpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvvegaspflvr

    Sequence, based on observed residues (ATOM records): (download) 
    >5aj9B (B:)
krpnflvivaddlgfsdigafggeiatpnldalaiaglrltdfhtasaxsptrsmlltgt
dhhiaglgtvaealtpelegkpgyeghlnervvalpellreagyqtlmagkwhlglkpeq
tphargfersfsllpgaanhygfeppydestprilkgtpalyvederyldtlpegfyssd
afgdkllqylkerdqsrpffaylpfsaphwplqapreivekyrgrydagpealrqerlar
lkelglveadveahpvlaltrewealedeeraksaramevyaamvermdwnigrvvdylr
rqgeldntfvlfmsdngaegalleafpkfgpdlldfldrhydnslenigsansyvwygpr
waqaatapsrlykafttqggirvpalvryprlsrqgaishafatvmdvtptlldlagvrh
pgkrwrgreiaeprgrswlgwlsgeteaahdentvtgwglfgmrairqgdwkavylpapv
gpatwqlydlardpgeihdladsqpgklaeliehwkryvsdtgvve