PDB entry 5act

View 5act on RCSB PDB site
Description: W228S-Investigation of the impact from residues W228 and Y233 in the metallo-beta-lactamase GIM-1
Class: hydrolase
Keywords: hydrolase, metallo-beta-lactamase, gim-1, carbapenemase, enzyme kinetics, mic,
Deposited on 2015-08-17, released 2015-12-23
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-12-23, with a file datestamp of 2015-12-18.
Experiment type: XRAY
Resolution: 1.81 Å
R-factor: N/A
AEROSPACI score: 0.36 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: gim-1 protein
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q704V1
      • engineered mutation (181)
    Domains in SCOPe 2.06: d5acta_
  • Chain 'B':
    Compound: gim-1 protein
    Species: Pseudomonas aeruginosa [TaxId:287]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q704V1
      • engineered mutation (181)
    Domains in SCOPe 2.06: d5actb_
  • Heterogens: ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >5actA (A:)
mknvlvflillvalpalaqghkplevikiedgvylhtsfkniegyglvdsnglvvldnnq
ayiidtpwseedtklllswatdrgyqvmasisthshedrtagikllnsksiptytseltk
kllaregkpvpthyfkddeftlgnglielyypgaghtednivawlpkskilfggclvrsh
eseglgyvgdasisswadsiknivskkypiqmvvpghgkvgssdildhtidlaesasnkl
mqptaeasad

    Sequence, based on observed residues (ATOM records): (download) 
    >5actA (A:)
hkplevikiedgvylhtsfkniegyglvdsnglvvldnnqayiidtpwseedtklllswa
tdrgyqvmasisthshedrtagikllnsksiptytseltkkllaregkpvpthyfkddef
tlgnglielyypgaghtednivawlpkskilfggclvrsheseglgyvgdasisswadsi
knivskkypiqmvvpghgkvgssdildhtidlaesasnk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >5actB (B:)
mknvlvflillvalpalaqghkplevikiedgvylhtsfkniegyglvdsnglvvldnnq
ayiidtpwseedtklllswatdrgyqvmasisthshedrtagikllnsksiptytseltk
kllaregkpvpthyfkddeftlgnglielyypgaghtednivawlpkskilfggclvrsh
eseglgyvgdasisswadsiknivskkypiqmvvpghgkvgssdildhtidlaesasnkl
mqptaeasad

    Sequence, based on observed residues (ATOM records): (download) 
    >5actB (B:)
ghkplevikiedgvylhtsfkniegyglvdsnglvvldnnqayiidtpwseedtklllsw
atdrgyqvmasisthshedrtagikllnsksiptytseltkkllaregkpvpthyfkdde
ftlgnglielyypgaghtednivawlpkskilfggclvrsheseglgyvgdasisswads
iknivskkypiqmvvpghgkvgssdildhtidlaesasn