PDB entry 4zk4

View 4zk4 on RCSB PDB site
Description: Crystal structure of a chimeric acetylcholine binding protein from Aplysia californica (Ac-AChBP) containing loop C from the human alpha 3 nicotinic acetylcholine receptor in complex with 7-(5-isopropoxy-pyridin-3-yl)-1-methyl-1,7-diaza-spiro[4.4]nonane
Class: acetylcholine binding protein
Keywords: AChBP, nicotinic, receptor, acetylcholine
Deposited on 2015-04-29, released 2015-05-13
The last revision prior to the SCOPe 2.06 freeze date was dated 2015-05-13, with a file datestamp of 2015-05-08.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: N/A
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica [TaxId:6500]
    Gene: CHRNA3, NACHRA3
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (8-188)
      • expression tag (3-7)
      • engineered mutation (62)
    • Uniprot P32297 (189-204)
    • Uniprot Q8WSF8 (205-End)
      • engineered mutation (206)
    Domains in SCOPe 2.06: d4zk4a1, d4zk4a2
  • Chain 'B':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica [TaxId:6500]
    Gene: CHRNA3, NACHRA3
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (8-188)
      • expression tag (6-7)
      • engineered mutation (62)
    • Uniprot P32297 (189-204)
    • Uniprot Q8WSF8 (205-End)
      • engineered mutation (206)
    Domains in SCOPe 2.06: d4zk4b1, d4zk4b2
  • Chain 'C':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica [TaxId:6500]
    Gene: CHRNA3, NACHRA3
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (8-188)
      • expression tag (7)
      • engineered mutation (62)
    • Uniprot P32297 (189-204)
    • Uniprot Q8WSF8 (205-End)
      • engineered mutation (206)
    Domains in SCOPe 2.06: d4zk4c1, d4zk4c2
  • Chain 'D':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica [TaxId:6500]
    Gene: CHRNA3, NACHRA3
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (8-188)
      • expression tag (7)
      • engineered mutation (62)
    • Uniprot P32297 (189-204)
    • Uniprot Q8WSF8 (205-End)
      • engineered mutation (206)
    Domains in SCOPe 2.06: d4zk4d1, d4zk4d2
  • Chain 'E':
    Compound: soluble acetylcholine receptor, neuronal acetylcholine receptor subunit alpha-3 chimera
    Species: Aplysia californica [TaxId:6500]
    Gene: CHRNA3, NACHRA3
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q8WSF8 (8-188)
      • expression tag (0-7)
      • engineered mutation (62)
    • Uniprot P32297 (189-204)
    • Uniprot Q8WSF8 (205-End)
      • engineered mutation (206)
    Domains in SCOPe 2.06: d4zk4e1, d4zk4e2
  • Heterogens: MG, SO4, PG4, TII, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4zk4A (A:)
ykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevdl
vyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthd
gsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyass
kyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >4zk4A (A:)
dddklhsqanlmrlksdlfnypgptkddpltvtlgftlqdivkadsstnevdlvyweqqr
wklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfip
aqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsa
tqykhdikyncceeiypdvvlvvkfrerrag

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4zk4B (B:)
ykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevdl
vyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthd
gsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyass
kyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >4zk4B (B:)
klhsqanlmrlksdlfnypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwkl
nslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqr
lsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqy
khdikyncceeiypdvvlvvkfrer

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4zk4C (C:)
ykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevdl
vyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthd
gsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyass
kyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >4zk4C (C:)
lhsqanlmrlksdlfnypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwkln
slmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqrl
sfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqyk
hdikyncceeiypdvvlvvkfre

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4zk4D (D:)
ykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevdl
vyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthd
gsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyass
kyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >4zk4D (D:)
lhsqanlmrlksdlfypgptkddpltvtlgftlqdivkadsstnevdlvyweqqrwklns
lmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsvmfipaqrls
fmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskyeilsatqykh
dikyncceeiypdvvlvvkfrerr

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4zk4E (E:)
ykddddklhsqanlmrlksdlfnrspmypgptkddpltvtlgftlqdivkadsstnevdl
vyweqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthd
gsvmfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyass
kyeilsatqykhdikyncceeiypdvvlvvkfrerragngffrnlfdsr

    Sequence, based on observed residues (ATOM records): (download) 
    >4zk4E (E:)
ykddddklhsqanlmrlksdlfnrypgptkddpltvtlgftlqdivkadsstnevdlvyw
eqqrwklnslmwdpneygnitdfrtsaadiwtpditaysstrpvqvlspqiavvthdgsv
mfipaqrlsfmcdptgvdseegatcavkfgswvysgfeidlktdtdqvdlssyyasskye
ilsatqykhdikyncceeiypdvvlvvkfre