PDB entry 4zj3

View 4zj3 on RCSB PDB site
Description: Crystal structure of cephalexin bound acyl-enzyme intermediate of Val216AcrF mutant TEM1 beta-lactamase from Escherichia coli: E166N and V216AcrF mutant.
Class: hydrolase
Keywords: noncanonical amino acid, beta-lactamase, protein evolution, evolutionary advantage, HYDROLASE
Deposited on 2015-04-29, released 2015-05-20
The last revision prior to the SCOPe 2.07 freeze date was dated 2015-06-17, with a file datestamp of 2015-06-12.
Experiment type: XRAY
Resolution: 1.7 Å
R-factor: N/A
AEROSPACI score: 0.4 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Beta-lactamase TEM
    Species: Escherichia coli [TaxId:562]
    Gene: bla, blaT-3, blaT-4, blaT-5, blaT-6
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62593 (Start-285)
      • engineered mutation (67)
      • see remark 999 (81)
      • engineered mutation (163)
      • see remark 999 (181)
      • engineered mutation (213)
      • expression tag (286-287)
    Domains in SCOPe 2.07: d4zj3a1, d4zj3a2
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4zj3A (A:)
msiqhfrvalipffaafclpvfahpetlvkvkdaedqlgarvgyieldlnsgkilesfrp
eerfpmmxtfkvllcgavlsridagqeqlgrrihysqndlveyspvtekhltdgmtvrel
csaaitmsdntaanlllttiggpkeltaflhnmgdhvtrldrwnpelneaipnderdttm
pvamattlrklltgelltlasrqqlidwmeadkxagpllrsalpagwfiadksgagergs
rgiiaalgpdgkpsrivviyttgsqatmdernrqiaeigaslikhwaaalehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4zj3A (A:)
hpetlvkvkdaedqlgarvgyieldlnsgkilesfrpeerfpmmxtfkvllcgavlsrid
agqeqlgrrihysqndlveyspvtekhltdgmtvrelcsaaitmsdntaanlllttiggp
keltaflhnmgdhvtrldrwnpelneaipnderdttmpvamattlrklltgelltlasrq
qlidwmeadkxagpllrsalpagwfiadksgagergsrgiiaalgpdgkpsrivviyttg
sqatmdernrqiaeigaslikhwaa