SCOPe 2.06: Structural Classification of Proteins

PDB entry 4wg2

View 4wg2 on RCSB PDB site
Description: P411BM3-CIS T438S I263F regioselective C-H amination catalyst
Class: oxidoreductase
Keywords: P411BM3-CIS, engineering, catalysis, OXIDOREDUCTASE
Deposited on 2014-09-17, released 2014-11-05
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-11-19, with a file datestamp of 2014-11-14.
Experiment type: XRAY
Resolution: 2.66 Å
R-factor: 0.199
AEROSPACI score: 0.23 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: Bifunctional P-450/NADPH-P450 reductase
Species: Bacillus megaterium [TaxId:1404]
Gene: CYP102A1, cyp102
Database cross-references and differences (RAF-indexed):
- Uniprot P14779 (Start-462)
  - engineered mutation (77)
  - engineered mutation (86)
  - engineered mutation (141)
  - engineered mutation (174)
  - engineered mutation (183)
  - engineered mutation (225)
  - engineered mutation (235)
  - engineered mutation (251)
  - engineered mutation (262)
  - engineered mutation (267)
  - engineered mutation (289)
  - engineered mutation (352)
  - engineered mutation (365)
  - engineered mutation (399)
  - engineered mutation (437)
  - engineered mutation (441)
  - expression tag (463)
Domains in SCOPe 2.06: d4wg2a1, d4wg2a2
Chain 'B':
Compound: Bifunctional P-450/NADPH-P450 reductase
Species: Bacillus megaterium [TaxId:1404]
Gene: CYP102A1, cyp102
Database cross-references and differences (RAF-indexed):
- Uniprot P14779
  - engineered mutation (77)
  - engineered mutation (86)
  - engineered mutation (141)
  - engineered mutation (174)
  - engineered mutation (183)
  - engineered mutation (235)
  - engineered mutation (251)
  - engineered mutation (262)
  - engineered mutation (267)
  - engineered mutation (289)
  - engineered mutation (352)
  - engineered mutation (365)
  - engineered mutation (399)
  - engineered mutation (437)
  - engineered mutation (441)
Domains in SCOPe 2.06: d4wg2b_
Chain 'C':
Compound: Bifunctional P-450/NADPH-P450 reductase
Species: Bacillus megaterium [TaxId:1404]
Gene: CYP102A1, cyp102
Database cross-references and differences (RAF-indexed):
- Uniprot P14779 (Start-462)
  - engineered mutation (77)
  - engineered mutation (86)
  - engineered mutation (141)
  - engineered mutation (174)
  - engineered mutation (183)
  - engineered mutation (225)
  - engineered mutation (235)
  - engineered mutation (251)
  - engineered mutation (262)
  - engineered mutation (267)
  - engineered mutation (289)
  - engineered mutation (352)
  - engineered mutation (365)
  - engineered mutation (399)
  - engineered mutation (437)
  - engineered mutation (441)
Domains in SCOPe 2.06: d4wg2c_
Heterogens: SO4, HEM, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>4wg2A (A:)
tikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlike
acdesrfdknlsqalkfardfagdglvtswtheknwkkahnillpsfsqqamkgyhammv
diavqlvqkwerlnadehievsedmtrltldtiglcgfnyrfnsfyrdqphpfiismvra
ldevmnklqranpddpaydenkrqfqedikvmndlvdkiiadrkargeqsddlltqmlng
kdpetgeplddgniryqiitflfagheatsgllsfalyflvknphvlqkvaeeaarvlvd
pvpsykqvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdevmvlipql
hrdktvwgddveefrperfenpsaipqhafkpfgngqrasigqqfalheatlvlgmmlkh
fdfedhtnyeldiketlslkpkgfvvkakskkiplggipspsthhhhhh

Sequence, based on observed residues (ATOM records): (download)

>4wg2A (A:)
empqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlikeacd
esrfdknlsqalkfardfagdglvtswtheknwkkahnillpsfsqqamkgyhammvdia
vqlvqkwerlnadehievsedmtrltldtiglcgfnyrfnsfyrdqphpfiismvralde
vmnklqranpddpaydenkrqfqedikvmndlvdkiiadrkargeqsddlltqmlngkdp
etgeplddgniryqiitflfagheatsgllsfalyflvknphvlqkvaeeaarvlvdpvp
sykqvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdevmvlipqlhrd
ktvwgddveefrperfenpsaipqhafkpfgngqrasigqqfalheatlvlgmmlkhfdf
edhtnyeldiketlslkpkgfvvkakskkiplggipspsth

Chain 'B':
Sequence, based on SEQRES records: (download)

>4wg2B (B:)
tikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlike
acdesrfdknlsqalkfardfagdglvtswtheknwkkahnillpsfsqqamkgyhammv
diavqlvqkwerlnadehievsedmtrltldtiglcgfnyrfnsfyrdqphpfiismvra
ldevmnklqranpddpaydenkrqfqedikvmndlvdkiiadrkargeqsddlltqmlng
kdpetgeplddgniryqiitflfagheatsgllsfalyflvknphvlqkvaeeaarvlvd
pvpsykqvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdevmvlipql
hrdktvwgddveefrperfenpsaipqhafkpfgngqrasigqqfalheatlvlgmmlkh
fdfedhtnyeldiketlslkpkgfvvkakskkiplggipspsthhhhhh

Sequence, based on observed residues (ATOM records): (download)

>4wg2B (B:)
kempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlikeac
desrfdknlsqalkfardfagdglvtswtheknwkkahnillpsfsqqamkgyhammvdi
avqlvqkwerlnadehievsedmtrltldtiglcgfnyrfnsfyrdqphpfiismvrald
evmnklqranpaydenkrqfqedikvmndlvdkiiadrkaeqsddlltqmlngkdpetge
plddgniryqiitflfagheatsgllsfalyflvknphvlqkvaeeaarvlvdpvpsykq
vkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdevmvlipqlhrdktvw
gddveefrperfenpsaipqhafkpfgngqrasigqqfalheatlvlgmmlkhfdfedht
nyeldiketlslkpkgfvvkakskkiplggi

Chain 'C':
Sequence, based on SEQRES records: (download)

>4wg2C (C:)
tikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlike
acdesrfdknlsqalkfardfagdglvtswtheknwkkahnillpsfsqqamkgyhammv
diavqlvqkwerlnadehievsedmtrltldtiglcgfnyrfnsfyrdqphpfiismvra
ldevmnklqranpddpaydenkrqfqedikvmndlvdkiiadrkargeqsddlltqmlng
kdpetgeplddgniryqiitflfagheatsgllsfalyflvknphvlqkvaeeaarvlvd
pvpsykqvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdevmvlipql
hrdktvwgddveefrperfenpsaipqhafkpfgngqrasigqqfalheatlvlgmmlkh
fdfedhtnyeldiketlslkpkgfvvkakskkiplggipspsthhhhhh

Sequence, based on observed residues (ATOM records): (download)

>4wg2C (C:)
empqpktfgelknlpllntdkpvqalmkiadelgeifkfeapgrvtrylssqrlikeacd
esrfdknlsqalkfardfagdglvtswtheknwkkahnillpsfsqqamkgyhammvdia
vqlvqkwerlnadehievsedmtrltldtiglcgfnyrfnsfyrdqphpfiismvralde
vmnklqranpddpaydenkrqfqedikvmndlvdkiiadrkargeqsddlltqmlngkdp
etgeplddgniryqiitflfagheatsgllsfalyflvknphvlqkvaeeaarvlvdpvp
sykqvkqlkyvgmvlnealrlwptapafslyakedtvlggeyplekgdevmvlipqlhrd
ktvwgddveefrperfenpsaipqhafkpfgngqrasigqqfalheatlvlgmmlkhfdf
edhtnyeldiketlslkpkgfvvkakskkiplggipspst