PDB entry 4uwt

View 4uwt on RCSB PDB site
Description: Hypocrea jecorina Cel7A E212Q mutant in complex with p-nitrophenyl cellobioside
Class: hydrolase
Keywords: hydrolase, glycoside hydrolase, cellulase, enzyme kinetics, non-productive binding
Deposited on 2014-08-14, released 2015-08-26
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-06-29.
Experiment type: XRAY
Resolution: 1.2 Å
R-factor: N/A
AEROSPACI score: 0.72 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: cellobiohydrolase cel7a
    Species: TRICHODERMA REESEI QM9414 [TaxId:334564]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P62694 (0-433)
      • cloning artifact (93)
      • engineered mutation (211)
    Domains in SCOPe 2.08: d4uwta_
  • Heterogens: NAG, CO, GOL, NPO, PEG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4uwtA (A:)
esactlqsethppltwqkcssggtctqqtgsvvidanwrwthatnsstncydgntwsstl
cpdnetcaknccldgaayastygvttsgnslsidfvtqsaqknvgarlylmasdttyqef
tllgnefsfdvdvsqlpcglngalyfvsmdadggvskyptntagakygtgycdsqcprdl
kfingqanvegwepssnnantgigghgsccsqmdiweansisealtphpcttvgqeiceg
dgcggtysdnryggtcdpdgcdwnpyrlgntsfygpgssftldttkkltvvtqfetsgai
nryyvqngvtfqqpnaelgsysgnelnddyctaeeaefggssfsdkggltqfkkatsggm
vlvmslwddyyanmlwldstyptnetsstpgavrgscstssgvpaqvesqspnakvtfsn
ikfgpigstgnpsg