PDB entry 4upd

View 4upd on RCSB PDB site
Description: Open conformation of O. piceae sterol esterase mutant I544W
Class: hydrolase
Keywords: hydrolase
Deposited on 2014-06-16, released 2014-09-24
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-09-24, with a file datestamp of 2014-09-19.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: 0.1988
AEROSPACI score: 0.27 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: sterol esterase
    Species: OPHIOSTOMA PICEAE [TaxId:61273]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q2TFW1 (8-544)
      • expression tag (7)
      • engineered mutation (539)
    Domains in SCOPe 2.06: d4upda1, d4upda2
  • Chain 'B':
    Compound: sterol esterase
    Species: OPHIOSTOMA PICEAE [TaxId:61273]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q2TFW1 (8-544)
      • engineered mutation (539)
    Domains in SCOPe 2.06: d4updb_
  • Heterogens: 7P9, PGE, NAG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4updA (A:)
eaeayvefttvnvnypegevvgvsvlgiesfrgvpfaqppvgnlrlkppvrytenigtkd
ttgigpscpqmylstgngellfqlvgnliniplfqtatlssedcltlniqrpagttsnss
lpvlfwifgggfelgtnqyydgidlltegislgepfifvainyrvggfgflggkeikadg
ssnlglldqrialewvadniasfggdpskvtiwgesagsisvfdqmalyggnnkykgkal
frggimnsgsvvpaapvdgvkaqaiydhvvseagcagtsdtlaclrtvdytkfltavnsv
pgivsyssialsylprpdgvvlidspeeivknkqyaavpmiigdqedegtlfavlpnnit
stakivqyfqdlyfynatkeqltafvntyptditagspfntgifnelypgfkrlaailgd
mtftlarraflqlcsevnpdvpswsylasydygfpflgtfhatdilqvfygvlpnyasgs
iqkyyinfvttgdpnkgaavdiqwpqwsakknilqiyatkavivadnfraksyeylynnw
gifri

    Sequence, based on observed residues (ATOM records): (download) 
    >4updA (A:)
fttvnvnypegevvgvsvlgiesfrgvpfaqppvgnlrlkppvrytenigtkdttgigps
cpqmylstgngellfqlvgnliniplfqtatlssedcltlniqrpagttsnsslpvlfwi
fgggfelgtnqyydgidlltegislgepfifvainyrvggfgflggkeikadgssnlgll
dqrialewvadniasfggdpskvtiwgesagsisvfdqmalyggnnkykgkalfrggimn
sgsvvpaapvdgvkaqaiydhvvseagcagtsdtlaclrtvdytkfltavnsvpgivsys
sialsylprpdgvvlidspeeivknkqyaavpmiigdqedegtlfavlpnnitstakivq
yfqdlyfynatkeqltafvntyptditagspfntgifnelypgfkrlaailgdmtftlar
raflqlcsevnpdvpswsylasydygfpflgtfhatdilqvfygvlpnyasgsiqkyyin
fvttgdpnkgaavdiqwpqwsakknilqiyatkavivadnfraksyeylynnwgifri

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4updB (B:)
eaeayvefttvnvnypegevvgvsvlgiesfrgvpfaqppvgnlrlkppvrytenigtkd
ttgigpscpqmylstgngellfqlvgnliniplfqtatlssedcltlniqrpagttsnss
lpvlfwifgggfelgtnqyydgidlltegislgepfifvainyrvggfgflggkeikadg
ssnlglldqrialewvadniasfggdpskvtiwgesagsisvfdqmalyggnnkykgkal
frggimnsgsvvpaapvdgvkaqaiydhvvseagcagtsdtlaclrtvdytkfltavnsv
pgivsyssialsylprpdgvvlidspeeivknkqyaavpmiigdqedegtlfavlpnnit
stakivqyfqdlyfynatkeqltafvntyptditagspfntgifnelypgfkrlaailgd
mtftlarraflqlcsevnpdvpswsylasydygfpflgtfhatdilqvfygvlpnyasgs
iqkyyinfvttgdpnkgaavdiqwpqwsakknilqiyatkavivadnfraksyeylynnw
gifri

    Sequence, based on observed residues (ATOM records): (download) 
    >4updB (B:)
ttvnvnypegevvgvsvlgiesfrgvpfaqppvgnlrlkppvrytenigtkdttgigpsc
pqmylstgngellfqlvgnliniplfqtatlssedcltlniqrpagttsnsslpvlfwif
gggfelgtnqyydgidlltegislgepfifvainyrvggfgflggkeikadgssnlglld
qrialewvadniasfggdpskvtiwgesagsisvfdqmalyggnnkykgkalfrggimns
gsvvpaapvdgvkaqaiydhvvseagcagtsdtlaclrtvdytkfltavnsvpgivsyss
ialsylprpdgvvlidspeeivknkqyaavpmiigdqedegtlfavlpnnitstakivqy
fqdlyfynatkeqltafvntyptditagspfntgifnelypgfkrlaailgdmtftlarr
aflqlcsevnpdvpswsylasydygfpflgtfhatdilqvfygvlpnyasgsiqkyyinf
vttgdpnkgaavdiqwpqwsakknilqiyatkavivadnfraksyeylynnwgifri