PDB entry 4ucw

View 4ucw on RCSB PDB site
Description: Structure of the T18V small subunit mutant of D. fructosovorans NiFe- hydrogenase
Class: oxidoreductase
Keywords: oxidoreductase, nife-hydrogenase mutant, unready state
Deposited on 2014-12-04, released 2015-02-18
The last revision prior to the SCOPe 2.08 freeze date was dated 2019-11-20, with a file datestamp of 2019-11-15.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: N/A
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Hydrogenase (NiFe) small subunit HydA
    Species: DESULFOVIBRIO FRUCTOSIVORANS [TaxId:878]
    Database cross-references and differences (RAF-indexed):
    • Uniprot E1K248 (Start-263)
      • engineered mutation (17)
    Domains in SCOPe 2.08: d4ucwa_
  • Chain 'B':
    Compound: Hydrogenase (NiFe) small subunit HydA
    Species: DESULFOVIBRIO FRUCTOSIVORANS [TaxId:878]
    Database cross-references and differences (RAF-indexed):
    • Uniprot E1K248 (Start-263)
      • engineered mutation (17)
    Domains in SCOPe 2.08: d4ucwb_
  • Chain 'C':
    Compound: Hydrogenase (NiFe) small subunit HydA
    Species: DESULFOVIBRIO FRUCTOSIVORANS [TaxId:878]
    Database cross-references and differences (RAF-indexed):
    • Uniprot E1K248 (Start-263)
      • engineered mutation (17)
    Domains in SCOPe 2.08: d4ucwc_
  • Chain 'Q':
    Compound: Nickel-dependent hydrogenase large subunit
    Species: DESULFOVIBRIO FRUCTOSIVORANS [TaxId:878]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4ucwq_
  • Chain 'R':
    Compound: Nickel-dependent hydrogenase large subunit
    Species: DESULFOVIBRIO FRUCTOSIVORANS [TaxId:878]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4ucwr_
  • Chain 'S':
    Compound: Nickel-dependent hydrogenase large subunit
    Species: DESULFOVIBRIO FRUCTOSIVORANS [TaxId:878]
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4ucws_
  • Heterogens: GOL, SF4, F3S, FCO, NI, MG, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4ucwA (A:)
ltakhrpsvvwlhnaecvgcteaairtikpyidalildtisldyqetimaaageaaeaal
hqalegkdgyylvvegglptidggqwgmvaghpmiettkkaaakakgiicigtcsayggv
qkakpnpsqakgvsealgvktinipgcppnpinfvgavvhvltkgipdldengrpklfyg
elvhdncprlphfeasefapsfdseeakkgfclyelgckgpvtynncpkvlfnqvnwpvq
aghpclgcsepdfwdtmtpfyeqg

    Sequence, based on observed residues (ATOM records): (download) 
    >4ucwA (A:)
takhrpsvvwlhnaecvgcteaairtikpyidalildtisldyqetimaaageaaeaalh
qalegkdgyylvvegglptidggqwgmvaghpmiettkkaaakakgiicigtcsayggvq
kakpnpsqakgvsealgvktinipgcppnpinfvgavvhvltkgipdldengrpklfyge
lvhdncprlphfeasefapsfdseeakkgfclyelgckgpvtynncpkvlfnqvnwpvqa
ghpclgcsepdfwdtmtpfyeqg

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4ucwB (B:)
ltakhrpsvvwlhnaecvgcteaairtikpyidalildtisldyqetimaaageaaeaal
hqalegkdgyylvvegglptidggqwgmvaghpmiettkkaaakakgiicigtcsayggv
qkakpnpsqakgvsealgvktinipgcppnpinfvgavvhvltkgipdldengrpklfyg
elvhdncprlphfeasefapsfdseeakkgfclyelgckgpvtynncpkvlfnqvnwpvq
aghpclgcsepdfwdtmtpfyeqg

    Sequence, based on observed residues (ATOM records): (download) 
    >4ucwB (B:)
hrpsvvwlhnaecvgcteaairtikpyidalildtisldyqetimaaageaaeaalhqal
egkdgyylvvegglptidggqwgmvaghpmiettkkaaakakgiicigtcsayggvqkak
pnpsqakgvsealgvktinipgcppnpinfvgavvhvltkgipdldengrpklfygelvh
dncprlphfeasefapsfdseeakkgfclyelgckgpvtynncpkvlfnqvnwpvqaghp
clgcsepdfwdtmtpfyeqg

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4ucwC (C:)
ltakhrpsvvwlhnaecvgcteaairtikpyidalildtisldyqetimaaageaaeaal
hqalegkdgyylvvegglptidggqwgmvaghpmiettkkaaakakgiicigtcsayggv
qkakpnpsqakgvsealgvktinipgcppnpinfvgavvhvltkgipdldengrpklfyg
elvhdncprlphfeasefapsfdseeakkgfclyelgckgpvtynncpkvlfnqvnwpvq
aghpclgcsepdfwdtmtpfyeqg

    Sequence, based on observed residues (ATOM records): (download) 
    >4ucwC (C:)
khrpsvvwlhnaecvgcteaairtikpyidalildtisldyqetimaaageaaeaalhqa
legkdgyylvvegglptidggqwgmvaghpmiettkkaaakakgiicigtcsayggvqka
kpnpsqakgvsealgvktinipgcppnpinfvgavvhvltkgipdldengrpklfygelv
hdncprlphfeasefapsfdseeakkgfclyelgckgpvtynncpkvlfnqvnwpvqagh
pclgcsepdfwdtmtpfyeqg

  • Chain 'Q':
    Sequence, based on SEQRES records: (download) 
    >4ucwQ (Q:)
aswshpqfekgasgaaeskptpqstftgpivvdpitrieghlrimvevengkvkdawsss
qlfrgleiilkgrdprdaqhftqracgvctyvhalassrcvddavkvsipanarmmrnlv
masqylhdhlvhfyhlhaldwvdvtaalkadpnkaaklaasiaparpgnsakalkavqdk
lkafvesgqlgiftnayflgghkayylppevdliatahylealhmqvkaasamailggkn
phtqftvvggcsnyqgltkdplanylalskevcqfvnecyipdllavagfykdwggiggt
snylafgefatddsspekhlatsqfpsgvitgrdlgkvdnvdlgaiyedvkyswyapggd
gkhpydgvtdpkytklddkdhyswmkaprykgkamevgplartfiayakgqpdfkkvvdm
vlgklsvpatalhstlgrtaargietaivcanmekwikemadsgakdntlcakwempees
kgvgladaprgalshwirikgkkidnfqlvvpstwnlgprgaqgdkspveealigtpiad
pkrpveilrtvhafdpciacgvh

    Sequence, based on observed residues (ATOM records): (download) 
    >4ucwQ (Q:)
ptpqstftgpivvdpitrieghlrimvevengkvkdawsssqlfrgleiilkgrdprdaq
hftqracgvctyvhalassrcvddavkvsipanarmmrnlvmasqylhdhlvhfyhlhal
dwvdvtaalkadpnkaaklaasiaparpgnsakalkavqdklkafvesgqlgiftnayfl
gghkayylppevdliatahylealhmqvkaasamailggknphtqftvvggcsnyqgltk
dplanylalskevcqfvnecyipdllavagfykdwggiggtsnylafgefatddsspekh
latsqfpsgvitgrdlgkvdnvdlgaiyedvkyswyapggdgkhpydgvtdpkytklddk
dhyswmkaprykgkamevgplartfiayakgqpdfkkvvdmvlgklsvpatalhstlgrt
aargietaivcanmekwikemadsgakdntlcakwempeeskgvgladaprgalshwiri
kgkkidnfqlvvpstwnlgprgaqgdkspveealigtpiadpkrpveilrtvhafdpcia
cgvh

  • Chain 'R':
    Sequence, based on SEQRES records: (download) 
    >4ucwR (R:)
aswshpqfekgasgaaeskptpqstftgpivvdpitrieghlrimvevengkvkdawsss
qlfrgleiilkgrdprdaqhftqracgvctyvhalassrcvddavkvsipanarmmrnlv
masqylhdhlvhfyhlhaldwvdvtaalkadpnkaaklaasiaparpgnsakalkavqdk
lkafvesgqlgiftnayflgghkayylppevdliatahylealhmqvkaasamailggkn
phtqftvvggcsnyqgltkdplanylalskevcqfvnecyipdllavagfykdwggiggt
snylafgefatddsspekhlatsqfpsgvitgrdlgkvdnvdlgaiyedvkyswyapggd
gkhpydgvtdpkytklddkdhyswmkaprykgkamevgplartfiayakgqpdfkkvvdm
vlgklsvpatalhstlgrtaargietaivcanmekwikemadsgakdntlcakwempees
kgvgladaprgalshwirikgkkidnfqlvvpstwnlgprgaqgdkspveealigtpiad
pkrpveilrtvhafdpciacgvh

    Sequence, based on observed residues (ATOM records): (download) 
    >4ucwR (R:)
kptpqstftgpivvdpitrieghlrimvevengkvkdawsssqlfrgleiilkgrdprda
qhftqracgvctyvhalassrcvddavkvsipanarmmrnlvmasqylhdhlvhfyhlha
ldwvdvtaalkadpnkaaklaasiaparpgnsakalkavqdklkafvesgqlgiftnayf
lgghkayylppevdliatahylealhmqvkaasamailggknphtqftvvggcsnyqglt
kdplanylalskevcqfvnecyipdllavagfykdwggiggtsnylafgefatddsspek
hlatsqfpsgvitgrdlgkvdnvdlgaiyedvkyswyapggdgkhpydgvtdpkytkldd
kdhyswmkaprykgkamevgplartfiayakgqpdfkkvvdmvlgklsvpatalhstlgr
taargietaivcanmekwikemadsgakdntlcakwempeeskgvgladaprgalshwir
ikgkkidnfqlvvpstwnlgprgaqgdkspveealigtpiadpkrpveilrtvhafdpci
acgvh

  • Chain 'S':
    Sequence, based on SEQRES records: (download) 
    >4ucwS (S:)
aswshpqfekgasgaaeskptpqstftgpivvdpitrieghlrimvevengkvkdawsss
qlfrgleiilkgrdprdaqhftqracgvctyvhalassrcvddavkvsipanarmmrnlv
masqylhdhlvhfyhlhaldwvdvtaalkadpnkaaklaasiaparpgnsakalkavqdk
lkafvesgqlgiftnayflgghkayylppevdliatahylealhmqvkaasamailggkn
phtqftvvggcsnyqgltkdplanylalskevcqfvnecyipdllavagfykdwggiggt
snylafgefatddsspekhlatsqfpsgvitgrdlgkvdnvdlgaiyedvkyswyapggd
gkhpydgvtdpkytklddkdhyswmkaprykgkamevgplartfiayakgqpdfkkvvdm
vlgklsvpatalhstlgrtaargietaivcanmekwikemadsgakdntlcakwempees
kgvgladaprgalshwirikgkkidnfqlvvpstwnlgprgaqgdkspveealigtpiad
pkrpveilrtvhafdpciacgvh

    Sequence, based on observed residues (ATOM records): (download) 
    >4ucwS (S:)
kptpqstftgpivvdpitrieghlrimvevengkvkdawsssqlfrgleiilkgrdprda
qhftqracgvctyvhalassrcvddavkvsipanarmmrnlvmasqylhdhlvhfyhlha
ldwvdvtaalkadpnkaaklaasiaparpgnsakalkavqdklkafvesgqlgiftnayf
lgghkayylppevdliatahylealhmqvkaasamailggknphtqftvvggcsnyqglt
kdplanylalskevcqfvnecyipdllavagfykdwggiggtsnylafgefatddsspek
hlatsqfpsgvitgrdlgkvdnvdlgaiyedvkyswyapggdgkhpydgvtdpkytkldd
kdhyswmkaprykgkamevgplartfiayakgqpdfkkvvdmvlgklsvpatalhstlgr
taargietaivcanmekwikemadsgakdntlcakwempeeskgvgladaprgalshwir
ikgkkidnfqlvvpstwnlgprgaqgdkspveealigtpiadpkrpveilrtvhafdpci
acgvh