PDB entry 4rzt

View 4rzt on RCSB PDB site
Description: Lac repressor engineered to bind sucralose, sucralose-bound tetramer
Class: transcription
Keywords: sucralose, lacI, lac repressor, lactose operon repressor, protein design, allostery, Escherichia coli, TRANSCRIPTION
Deposited on 2014-12-24, released 2015-12-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 3.1 Å
R-factor: N/A
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3 (Start-380)
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rzta_
  • Chain 'B':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rztb_
  • Chain 'C':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3 (Start-380)
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rztc_
  • Chain 'D':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rztd_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4rztA (A:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rztA (A:)
qslligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrv
sgliinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalgh
qqiallagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivp
tamlvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqt
svdrllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrlesg
q

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4rztB (B:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rztB (B:)
lligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrvsg
liinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalghqq
iallagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivpta
mlvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqtsv
drllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrl

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4rztC (C:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rztC (C:)
qslligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrv
sgliinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalgh
qqiallagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivp
tamlvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqt
svdrllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrlesg
q

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4rztD (D:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rztD (D:)
lligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrvsg
liinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalghqq
iallagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivpta
mlvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqtsv
drllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrl