PDB entry 4rzs

View 4rzs on RCSB PDB site
Description: Lac repressor engineered to bind sucralose, unliganded tetramer
Class: transcription
Keywords: sucralose, lacI, lac repressor, lactose operon repressor, protein design, allostery, Escherichia coli, TRANSCRIPTION
Deposited on 2014-12-24, released 2015-12-23
The last revision prior to the SCOPe 2.08 freeze date was dated 2017-11-22, with a file datestamp of 2017-11-17.
Experiment type: XRAY
Resolution: 2.71 Å
R-factor: N/A
AEROSPACI score: 0.17 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rzsa1, d4rzsa2
  • Chain 'B':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rzsb1, d4rzsb2
  • Chain 'C':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rzsc_
  • Chain 'D':
    Compound: lac repressor
    Species: Escherichia coli [TaxId:536056]
    Gene: b0345, ECDH1ME8569_0332, EcDH1_3261, lacI
    Database cross-references and differences (RAF-indexed):
    • Uniprot C9QQT3
      • engineered mutation (169-170)
      • engineered mutation (176)
      • engineered mutation (213)
    Domains in SCOPe 2.08: d4rzsd_
  • Heterogens: GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4rzsA (A:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rzsA (A:)
pvtlydvaeyagvsyqtvsrvvnqashvsaktrekveaamaelnyipnrslligvatssl
alhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrvsgliinyplddq
daiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalghqqiallagplss
vdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivptamlvandqmal
gamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqtsvdrllqlsqgq
avkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrles

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4rzsB (B:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rzsB (B:)
pvtlydvaeyagvsyqtvsrvvnqashvsaktrekveaamaelnyipnrvaqqlagkqsl
ligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrvsgl
iinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalghqqi
allagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivptam
lvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqtsvd
rllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrle

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4rzsC (C:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rzsC (C:)
slligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrvs
gliinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalghq
qiallagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivpt
amlvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqts
vdrllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrles

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4rzsD (D:)
mgsshhhhhhssglvprgshmvkpvtlydvaeyagvsyqtvsrvvnqashvsaktrekve
aamaelnyipnrvaqqlagkqslligvatsslalhapsqivaaiksradqlgasvvvsmv
ersgveackaavhnllaqrvsgliinyplddqdaiaveaactnvpalfltasdqtplnsi
ifshedgtrlgvehlvalghqqiallagplssvdarlrlagwhkyltrnqiqpiaeregd
wsamsgfqqtmqmlnegivptamlvandqmalgamraitesglrvgadisvvgyddteds
scyipplttikqdfrllgqtsvdrllqlsqgqavkgnqllpvslvkrkttlapntqtasp
raladslmqlarqvsrlesgq

    Sequence, based on observed residues (ATOM records): (download) 
    >4rzsD (D:)
slligvatsslalhapsqivaaiksradqlgasvvvsmversgveackaavhnllaqrvs
gliinyplddqdaiaveaactnvpalfltasdqtplnsiifshedgtrlgvehlvalghq
qiallagplssvdarlrlagwhkyltrnqiqpiaeregdwsamsgfqqtmqmlnegivpt
amlvandqmalgamraitesglrvgadisvvgyddtedsscyipplttikqdfrllgqts
vdrllqlsqgqavkgnqllpvslvkrkttlapntqtaspraladslmqlarqvsrl