PDB entry 4rsn

View 4rsn on RCSB PDB site
Description: Crystal structure of the E267V mutant of cytochrome P450 BM3
Class: Oxidoreductase
Keywords: Bifunctional P-450/NADPH-P450 reductase, heme domain, Oxidoreductase
Deposited on 2014-11-09, released 2015-12-02
The last revision prior to the SCOPe 2.05 freeze date was dated 2015-12-02, with a file datestamp of 2015-11-27.
Experiment type: XRAY
Resolution: 2.7 Å
R-factor: N/A
AEROSPACI score: 0.18 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Bifunctional P-450/NADPH-P450 reductase
    Species: Bacillus megaterium [TaxId:1404]
    Gene: cyp102, cyp102A1, p450bm-3
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14779 (4-459)
      • expression tag (0-3)
      • engineered mutation (51)
      • engineered mutation (55)
      • engineered mutation (91)
      • engineered mutation (271)
      • conflict (319)
      • conflict (348)
      • engineered mutation (405)
    Domains in SCOPe 2.05: d4rsna_
  • Chain 'B':
    Compound: Bifunctional P-450/NADPH-P450 reductase
    Species: Bacillus megaterium [TaxId:1404]
    Gene: cyp102, cyp102A1, p450bm-3
    Database cross-references and differences (RAF-indexed):
    • Uniprot P14779 (4-459)
      • expression tag (0-3)
      • engineered mutation (51)
      • engineered mutation (55)
      • engineered mutation (91)
      • engineered mutation (271)
      • conflict (319)
      • conflict (348)
      • engineered mutation (405)
    Domains in SCOPe 2.05: d4rsnb_
  • Heterogens: HEM, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4rsnA (A:)
rgshmtikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapglvtrflssq
rlikeacdesrfdknlsqalkfvrdfagdglvtswtheknwkkahnillpsfsqqamkgy
hammvdiavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfit
smvraldeamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgeqsddllt
hmlngkdpetgeplddeniryqiitfliaghvttsgllsfalyflvknphvlqkaaeeaa
rvlvdpvpsykqvkqlkyvdmvlnealrlwptapafslyakedtvlggkyplekgdelmv
lipqlhrdktiwgddveefrperfenpsaipqhafkpfgngqracpgqqfalheatlvlg
mmlkhfdfedhtnyeldiketltlkpegfvvkakskkipl

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4rsnB (B:)
rgshmtikempqpktfgelknlpllntdkpvqalmkiadelgeifkfeapglvtrflssq
rlikeacdesrfdknlsqalkfvrdfagdglvtswtheknwkkahnillpsfsqqamkgy
hammvdiavqlvqkwerlnadehievpedmtrltldtiglcgfnyrfnsfyrdqphpfit
smvraldeamnklqranpddpaydenkrqfqedikvmndlvdkiiadrkasgeqsddllt
hmlngkdpetgeplddeniryqiitfliaghvttsgllsfalyflvknphvlqkaaeeaa
rvlvdpvpsykqvkqlkyvdmvlnealrlwptapafslyakedtvlggkyplekgdelmv
lipqlhrdktiwgddveefrperfenpsaipqhafkpfgngqracpgqqfalheatlvlg
mmlkhfdfedhtnyeldiketltlkpegfvvkakskkipl