PDB entry 4q73

View 4q73 on RCSB PDB site
Description: Crystal Structure of Bradyrhizobium japonicum Proline Utilization A (PutA) Mutant D778Y
Deposited on 2014-04-23, released 2014-08-06
The last revision was dated 2014-09-10, with a file datestamp of 2014-09-05.
Experiment type: XRAY
Resolution: 2.3 Å
R-factor: 0.198
AEROSPACI score: 0.3 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: proline dehydrogenase
    Species: Bradyrhizobium diazoefficiens [TaxId:224911]
    Gene: BJ6T_21270, blr7261, putA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q89E26
      • engineered mutation (779)
  • Chain 'B':
    Compound: proline dehydrogenase
    Species: Bradyrhizobium diazoefficiens [TaxId:224911]
    Gene: BJ6T_21270, blr7261, putA
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q89E26
      • engineered mutation (779)
  • Heterogens: FAD, SO4, GOL, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >4q73A (A:)
ghmpnipppftapyapddaeiaarllpashlsppqearihrtatrlieairkrddrlggv
edmlrefalstkeglalmvlaeallrvpdartadqfiedklgegdfihhetkstaflvna
sawalglsarviqpgetpdgtigrlvkrlgapavrtatrqamrlmgnhfvlgetieqale
rgkprsgqktrysfdmlgegartaadarryfdayasaietigkaagnhalpdrpgisvkl
salhprfeaisrarvmvelvpqlldlaqrakahdlnftvdaeeadrlelsldviaatlad
pslkgwdgfglaiqayqkrasavidyvdalarahdrklmvrlvkgaywdteikraqergl
dgypvftrkamtdlnyvacaskllalrprifpqfathnaltvatvlemaegssgfefqrl
hgmgealyeqlakdhadiayrtyapvgshrdllaylvrrllenganssfvaqaadyrvpv
pallqrpadaivrpqaaahpriplpcdlfaperrnsrgvefgartaldqlltdvkaetgd
lkpiadatpdqahaavaaaragfagwsrtpagiraaaleqaahllesrsahfiallqreg
gktlddalselreaadfcryyaaqgrklfgsetampgptgesnaltmrgrgvfvaispwn
fplaiflgqvtaalmagnsvvakpaeqtpriareavallheagipksalylvtgdgriga
altahpdiagvvftgstevarsinralaakdgpivpliaetgginamiadatalpeqvay
dvvtsafrsagqrcsalrllfvqedvadrmiemvagaarelkigdpsdvathvgpvidve
akqrldahiarmktearlhfagpapegcfvaphifelteagqlteevfgpilhvvryrpe
nlervlraiertgygltlgvhsriddsieaiidrvqvgniyvnrnmigavvgvqpfggng
lsgtgpkaggphylarfateqtvtintaaaggnaallagee

    Sequence, based on observed residues (ATOM records):
    >4q73A (A:)
nipppftapyapddaeiaarllpashlsppqearihrtatrlieairkrlggvedmlref
alstkeglalmvlaeallrvpdartadqfiedklgegdfihhetkstaflvnasawarvi
qpgetpdgtigrlvkrlgapavrtatrqamrlmgnhfvlgetieqalergkpgqktrysf
dmlgegartaadarryfdayasaietigkaagnhalpdrpgisvklsalhprfeaisrar
vmvelvpqlldlaqrakahdlnftvdaeeadrlelsldviaatladpslkgwdgfglaiq
ayqkrasavidyvdalarahdrklmvrlvkgaywdteikraqergldgypvftrkamtdl
nyvacaskllalrprifpqfathnaltvatvlemaegssgfefqrlhgmgealyeqlakd
hadiayrtyapvgshrdllaylvrrllenganssfvaqaadyrvpvpallqrpadaivrp
qaaahpriplpcdlfaperrnsrgvefgartaldqlltdvkaeiadatpdqahaavaaar
agfagwsrtpagiraaaleqaahllesrsahfiallqreggktlddalselreaadfcry
yaaqgrklfgsetampgptgesnaltmrgrgvfvaispwnfplaiflgqvtaalmagnsv
vakpaeqtpriareavallheagipksalylvtgdgrigaaltahpdiagvvftgsteva
rsinralaakdgpivpliaetgginamiadatalpeqvaydvvtsafrsagqrcsalrll
fvqedvadrmiemvagaarelkigdpsdvathvgpvidveakqrldahiarmktearlhf
agpapegcfvaphifelteagqlteevfgpilhvvryrpenlervlraiertgygltlgv
hsriddsieaiidrvqvgniyvnrnmigavvgvqpfggnglsgtgpkaggphylarfate
qtvtintaaag

  • Chain 'B':
    Sequence, based on SEQRES records:
    >4q73B (B:)
ghmpnipppftapyapddaeiaarllpashlsppqearihrtatrlieairkrddrlggv
edmlrefalstkeglalmvlaeallrvpdartadqfiedklgegdfihhetkstaflvna
sawalglsarviqpgetpdgtigrlvkrlgapavrtatrqamrlmgnhfvlgetieqale
rgkprsgqktrysfdmlgegartaadarryfdayasaietigkaagnhalpdrpgisvkl
salhprfeaisrarvmvelvpqlldlaqrakahdlnftvdaeeadrlelsldviaatlad
pslkgwdgfglaiqayqkrasavidyvdalarahdrklmvrlvkgaywdteikraqergl
dgypvftrkamtdlnyvacaskllalrprifpqfathnaltvatvlemaegssgfefqrl
hgmgealyeqlakdhadiayrtyapvgshrdllaylvrrllenganssfvaqaadyrvpv
pallqrpadaivrpqaaahpriplpcdlfaperrnsrgvefgartaldqlltdvkaetgd
lkpiadatpdqahaavaaaragfagwsrtpagiraaaleqaahllesrsahfiallqreg
gktlddalselreaadfcryyaaqgrklfgsetampgptgesnaltmrgrgvfvaispwn
fplaiflgqvtaalmagnsvvakpaeqtpriareavallheagipksalylvtgdgriga
altahpdiagvvftgstevarsinralaakdgpivpliaetgginamiadatalpeqvay
dvvtsafrsagqrcsalrllfvqedvadrmiemvagaarelkigdpsdvathvgpvidve
akqrldahiarmktearlhfagpapegcfvaphifelteagqlteevfgpilhvvryrpe
nlervlraiertgygltlgvhsriddsieaiidrvqvgniyvnrnmigavvgvqpfggng
lsgtgpkaggphylarfateqtvtintaaaggnaallagee

    Sequence, based on observed residues (ATOM records):
    >4q73B (B:)
nipppftapyapddaeiaarllpashlsppqearihrtatrlieairkrlggvedmlref
alstkeglalmvlaeallrvpdartadqfiedklgegdfihhetkstaflvnasawarvi
qpgetpdgtigrlvkrlgapavrtatrqamrlmgnhfvlgetieqalergkpqktrysfd
mlgegartaadarryfdayasaietigkaagnhalpdrpgisvklsalhprfeaisrarv
mvelvpqlldlaqrakahdlnftvdaeeadrlelsldviaatladpslkgwdgfglaiqa
yqkrasavidyvdalarahdrklmvrlvkgaywdteikraqergldgypvftrkamtdln
yvacaskllalrprifpqfathnaltvatvlemaegssgfefqrlhgmgealyeqlakdh
adiayrtyapvgshrdllaylvrrllenganssfvaqaadyrvpvpallqrpadaivrpq
aaahpriplpcdlfaperrnsrgvefgartaldqlltdvkaeiadatpdqahaavaaara
gfagwsrtpagiraaaleqaahllesrsahfiallqreggktlddalselreaadfcryy
aaqgrklfgsetampgptgesnaltmrgrgvfvaispwnfplaiflgqvtaalmagnsvv
akpaeqtpriareavallheagipksalylvtgdgrigaaltahpdiagvvftgstevar
sinralaakdgpivpliaetgginamiadatalpeqvaydvvtsafrsagqrcsalrllf
vqedvadrmiemvagaarelkigdpsdvathvgpvidveakqrldahiarmktearlhfa
gpapegcfvaphifelteagqlteevfgpilhvvryrpenlervlraiertgygltlgvh
sriddsieaiidrvqvgniyvnrnmigavvgvqpfggnglsgtgpkaggphylarfateq
tvtintaaag