PDB entry 4pem

View 4pem on RCSB PDB site
Description: crystal structure of s1g mutant of penicillin g acylase from kluyvera citrophila
Deposited on 2014-04-24, released 2015-07-22
The last revision was dated 2015-07-22, with a file datestamp of 2015-07-17.
Experiment type: XRAY
Resolution: 2.5 Å
R-factor: N/A
AEROSPACI score: 0.2 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Penicillin G acylase Alpha
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: Penicillin G acylase Beta
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: Penicillin G acylase Alpha
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: Penicillin G acylase Beta
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A068F6N5 (0-End)
      • engineered mutation (3)
  • Chain 'E':
    Compound: Penicillin G acylase Alpha
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: Penicillin G acylase Beta
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A068F6N5 (0-End)
      • engineered mutation (3)
  • Chain 'G':
    Compound: Penicillin G acylase Alpha
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
  • Chain 'H':
    Compound: Penicillin G acylase Beta
    Species: Kluyvera cryocrescens [TaxId:580]
    Database cross-references and differences (RAF-indexed):
    • Uniprot A0A068F6N5 (0-End)
      • engineered mutation (3)
  • Heterogens: CA, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >4pemA (A:)
mknrnrmivngivtsliccsslsalaaspptevkivrdeygmphiyaddtyrlfygygyv
vaqdrlfqmemarrstqgtvsevlgkafvsfdkdirqnywpdsiraqiaslsaedksilq
gyadgmnawidkvnaspdkllpqqfstfgfkpkhwepfdvamifvgtmanrfsdstseid
nlalltalkdkygkqqgmavfnqlkwlvnpsapttiaaresayplkfdlqntqtaallpr
ydqpapmldrpakgtdgallaltadqnretiaaqfaqsganglagy

    Sequence, based on observed residues (ATOM records):
    >4pemA (A:)
pptevivrdygmphiyaddylfygygyvvaqdrlfqmemarrstqgtvsevlgafvfdki
rnywpdsiraqiaslsaedksilqgyadgmnawdkvnaspllpqqfstfgfpkhwepfdv
amifvgtmanrfsdstsednlalltalkdkygkqqgmavfnqlkwlvnpsapttiaarsa
yplktaallprydpapmldrakgtdgallaltadqnretiaaqfaqsganglagy

  • Chain 'B':
    Sequence, based on SEQRES records:
    >4pemB (B:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqrlehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4pemB (B:)
ttgnmwvignkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvfgh
ngiswgstagfgddvdifaelakpgyyqhgewvmlsrketiavkdgqptftvwrtlhgnv
iktdtattayaarawdgkevasllawthqmkaknwpewtqqaakqaltinwyyadvngni
gyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyianwnnspqkdy
pasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlrlflpalkdata
nlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkrtvvaavpapfg
kwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggkpqqevilaald
dawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyqnrgtendmivf
sptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslwltpqdvdehke
sqevlqvqr

  • Chain 'C':
    Sequence, based on SEQRES records:
    >4pemC (C:)
mknrnrmivngivtsliccsslsalaaspptevkivrdeygmphiyaddtyrlfygygyv
vaqdrlfqmemarrstqgtvsevlgkafvsfdkdirqnywpdsiraqiaslsaedksilq
gyadgmnawidkvnaspdkllpqqfstfgfkpkhwepfdvamifvgtmanrfsdstseid
nlalltalkdkygkqqgmavfnqlkwlvnpsapttiaaresayplkfdlqntqtaallpr
ydqpapmldrpakgtdgallaltadqnretiaaqfaqsganglagy

    Sequence, based on observed residues (ATOM records):
    >4pemC (C:)
pptevkivrdeygmphiyaddtyrlfygygyvvaqdrlfqmemarrstqgtvsevlgkaf
vsfdkdirqnywpdsiraqiaslsaedksilqgyadgmnawidkvnaspdkllpqqfstf
gfkpkhwepfdvamifvgtmanrfsdstseidnlalltalkdkygkqqgmavfnqlkwlv
npsapttiaaresayplkfdlqntqtaallprydqpapmldrpakgtdgallaltadqnr
etiaaqfaqsganglagy

  • Chain 'D':
    Sequence, based on SEQRES records:
    >4pemD (D:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqrlehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4pemD (D:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqr

  • Chain 'E':
    Sequence, based on SEQRES records:
    >4pemE (E:)
mknrnrmivngivtsliccsslsalaaspptevkivrdeygmphiyaddtyrlfygygyv
vaqdrlfqmemarrstqgtvsevlgkafvsfdkdirqnywpdsiraqiaslsaedksilq
gyadgmnawidkvnaspdkllpqqfstfgfkpkhwepfdvamifvgtmanrfsdstseid
nlalltalkdkygkqqgmavfnqlkwlvnpsapttiaaresayplkfdlqntqtaallpr
ydqpapmldrpakgtdgallaltadqnretiaaqfaqsganglagy

    Sequence, based on observed residues (ATOM records):
    >4pemE (E:)
pptevkivrdeygmphiyaddtyrlfygygyvvaqdrlfqmemarrstqgtvsevlgkaf
vsfdkdirqnywpdsiraqiaslsaedksilqgyadgmnawidkvnaspdkllpqqfstf
gfkpkhwepfdvamifvgtmanrfsdstseidnlalltalkdkygkqqgmavfnqlkwlv
npsapttiaaresayplkfdlqntqtaallprydqpapmldrpakgtdgallaltadqnr
etiaaqfaqsganglagy

  • Chain 'F':
    Sequence, based on SEQRES records:
    >4pemF (F:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqrlehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4pemF (F:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqr

  • Chain 'G':
    Sequence, based on SEQRES records:
    >4pemG (G:)
mknrnrmivngivtsliccsslsalaaspptevkivrdeygmphiyaddtyrlfygygyv
vaqdrlfqmemarrstqgtvsevlgkafvsfdkdirqnywpdsiraqiaslsaedksilq
gyadgmnawidkvnaspdkllpqqfstfgfkpkhwepfdvamifvgtmanrfsdstseid
nlalltalkdkygkqqgmavfnqlkwlvnpsapttiaaresayplkfdlqntqtaallpr
ydqpapmldrpakgtdgallaltadqnretiaaqfaqsganglagy

    Sequence, based on observed residues (ATOM records):
    >4pemG (G:)
pptevkivrdeygmphiyaddtyrlfygygyvvaqdrlfqmemarrstqgtvsevlgkaf
vsfdkdirqnywpdsiraqiaslsaedksilqgyadgmnawidkvnaspdkllpqqfstf
gfkpkhwepfdvamifvgtmanrfsdstseidnlalltalkdkygkqqgmavfnqlkwlv
npsapttiaaresayplkfdlqntqtaallprydqpapmldrpakgtdgallaltadqnr
etiaaqfaqsganglagy

  • Chain 'H':
    Sequence, based on SEQRES records:
    >4pemH (H:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqrlehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4pemH (H:)
pttgnmwvigknkaqdakaimvngpqfgwyapaytygiglhgagydvtgntpfaypglvf
ghngtiswgstagfgddvdifaeklsaekpgyyqhngewvkmlsrketiavkdgqpetft
vwrtlhgnviktdtatqtayakarawdgkevasllawthqmkaknwpewtqqaakqalti
nwyyadvngnigyvhtgaypdrqpghdprlpvpgtgkwdwkgllsfdlnpkvynpqsgyi
anwnnspqkdypasdlfaflwggadrvteidtildkqprftadqawdvirqtsrrdlnlr
lflpalkdatanlaendprrqlvdklaswdgenlvnddgktyqqpgsailnawltsmlkr
tvvaavpapfgkwysasgyettqdgptgslnisvgakilyealqgdkspipqavdlfggk
pqqevilaalddawqtlskrygndvtgwktpamaltfrannffgvpqaaakearhqaeyq
nrgtendmivfsptsgnrpvlawdvvapgqsgfiapdgkadkhyddqlkmyesfgrkslw
ltpqdvdehkesqevlqvqr