PDB entry 4oip

View 4oip on RCSB PDB site
Description: Crystal structure of Thermus thermophilus transcription initiation complex soaked with GE23077, ATP, and CMPcPP
Deposited on 2014-01-20, released 2014-05-07
The last revision was dated 2014-05-14, with a file datestamp of 2014-05-09.
Experiment type: XRAY
Resolution: 3.4 Å
R-factor: 0.209
AEROSPACI score: 0.15 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Thermus thermophilus [TaxId:300852]
    Database cross-references and differences (RAF-indexed):
  • Chain 'B':
    Compound: DNA-directed RNA polymerase subunit alpha
    Species: Thermus thermophilus [TaxId:300852]
    Database cross-references and differences (RAF-indexed):
  • Chain 'C':
    Compound: DNA-directed RNA polymerase subunit beta
    Species: Thermus thermophilus [TaxId:300852]
    Database cross-references and differences (RAF-indexed):
  • Chain 'D':
    Compound: DNA-directed RNA polymerase subunit beta'
    Species: Thermus thermophilus [TaxId:300852]
    Database cross-references and differences (RAF-indexed):
  • Chain 'E':
    Compound: DNA-directed RNA polymerase subunit omega
    Species: Thermus thermophilus [TaxId:300852]
    Database cross-references and differences (RAF-indexed):
  • Chain 'F':
    Compound: DNA directed RNA polymerase sigma factor A
    Species: Thermus thermophilus [TaxId:300852]
    Gene: rpoD, TTHA0532
    Database cross-references and differences (RAF-indexed):
  • Chain 'G':
    Compound: 5'-d(*cp*cp*tp*gp*cp*ap*tp*cp*cp*gp*tp*gp*ap*gp*tp*cp*gp*ap*gp*gp*g)-3'
  • Chain 'H':
    Compound: 5'-d(*tp*ap*tp*ap*ap*tp*gp*gp*gp*ap*gp*cp*tp*gp*tp*cp*ap*cp*gp*gp*ap*tp*gp*cp*ap*gp*g)-3'
  • Chain 'I':
    Compound: ge23077
    Species: ACTINOMADURA SP., synthetic [TaxId:1989]
    Database cross-references and differences (RAF-indexed):
    • PDB 4OIP (0-6)
  • Heterogens: MB8, MG, ZN, ATP, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >4oipA (A:)
mldsklkapvftvrtqgreygefvleplergfgvtlgnplrrillssipgtavtsvyied
vlhefstipgvkedvveiilnlkelvvrflnpslqtvtlllkaegpkevkardflpvadv
eimnpdlhiatleeggrlnmevrvdrgvgyvpaekhgikdrinaipvdavfspvrrvafq
vedtrlgqrtdldkltlriwtdgsvtplealnqaveilrehltyfsnpqaaavaapeeak
epeappeqeeeldlpleelglstrvlhslkeegiesvrallalnlkdlknipgigersle
eikealekkgftlke

    Sequence, based on observed residues (ATOM records):
    >4oipA (A:)
sklkapvftvrtqgreygefvleplergfgvtlgnplrrillssipgtavtsvyiedvlh
efstipgvkedvveiilnlkelvvrflnpslqtvtlllkaegpkevkardflpvadveim
npdlhiatleeggrlnmevrvdrgvgyvpaekhgikdrinaipvdavfspvrrvafqved
trlgqrtdldkltlriwtdgsvtplealnqaveilrehltyfsnpqaaava

  • Chain 'B':
    Sequence, based on SEQRES records:
    >4oipB (B:)
mldsklkapvftvrtqgreygefvleplergfgvtlgnplrrillssipgtavtsvyied
vlhefstipgvkedvveiilnlkelvvrflnpslqtvtlllkaegpkevkardflpvadv
eimnpdlhiatleeggrlnmevrvdrgvgyvpaekhgikdrinaipvdavfspvrrvafq
vedtrlgqrtdldkltlriwtdgsvtplealnqaveilrehltyfsnpqaaavaapeeak
epeappeqeeeldlpleelglstrvlhslkeegiesvrallalnlkdlknipgigersle
eikealekkgftlke

    Sequence, based on observed residues (ATOM records):
    >4oipB (B:)
ldsklkapvftvrtqgreygefvleplergfgvtlgnplrrillssipgtavtsvyiedv
lhefstipgvkedvveiilnlkelvvrflnpslqtvtlllkaegpkevkardflpvadve
imnpdlhiatleeggrlnmevrvdrgvgyvpaekhgikdrinaipvdavfspvrrvafqv
edtrlgqrtdldkltlriwtdgsvtplealnqaveilrehltyfsnp

  • Chain 'C':
    Sequence, based on SEQRES records:
    >4oipC (C:)
meikrfgrireviplpplteiqvesyrralqadvppekrenvgiqaafretfpieeedkg
kgglvldfleyrlgeppfpqdecrekdltyqaplyarlqlihkdtglikedevflghipl
mtedgsfiingadrvivsqihrspgvyftpdparpgryiasiiplpkrgpwidlevepng
vvsmkvnkrkfplvlllrvlgydqetlarelgaygelvqglmdesvfamrpeealirlft
llrpgdppkrdkavayvygliadprrydlgeagrykaeeklgirlsgrtlarfedgefkd
evflptlrylfaltagvpghevddidhlgnrrirtvgelmtdqfrvglarlargvrerml
mgsedsltpaklvnsrpleaaireffsrsqlsqfkdetnplsslrhkrrisalgpggltr
eragfdvrdvhrthygricpvetpeganiglitslaayarvdelgfirtpyrrvvggvvt
devvymtateedrytiaqantplegnriaaervvarrkgepvivspeevefmdvspkqvf
svntnlipflehddanralmgsnmqtqavpliraqapvvmtgleervvrdslaalyaeed
gevakvdgnrivvryedgrlveyplrrfyrsnqgtaldqrprvvvgqrvrkgdlladgpa
sengflalgqnvlvaimpfdgynfedaiviseellkrdfytsihieryeieardtklgpe
ritrdiphlseaalrdldeegvvrigaevkpgdilvgrtsfkgeseptpeerllrsifge
kardvkdtslrvppgeggivvrtvrlrrgdpgvelkpgvrevvrvyvaqkrklqvgdkla
nrhgnkgvvakilpvedmphlpdgtpvdvilnplgvpsrmnlgqilethlglagyflgqr
yispifdgakepeikellaqafevyfgkrkgegfgvdkrevevlrraeklglvtpgktpe
eqlkelflqgkvvlydgrtgepiegpivvgqmfimklyhmvedkmharstgpyslitqqp
lggkaqfggqrfgemevwaleaygaahtlqemltlksddiegrnaayeaiikgedvpeps
vpesfrvlvkelqalaldvqtldekdnpvdifeglaskr

    Sequence, based on observed residues (ATOM records):
    >4oipC (C:)
meikrfgrireviplpplteiqvesyrralqadvppekrenvgiqaafretfpieeglvl
dfleyrlgeppfpqdecrekdltyqaplyarlqlihkdtglikedevflghiplmtedgs
fiingadrvivsqihrspgvyftpdparpgryiasiiplpkrgpwidlevepngvvsmkv
nkrkfplvlllrvlgydqetlarelgaygelvqglmdesvfamrpeealirlftllrpgd
ppkrdkavayvygliadprrydlgeagrykaeeklgirlsgrtlarfedgefkdevflpt
lrylfaltagvpghevddidhlgnrrirtvgelmtdqfrvglarlargvrermlmgseds
ltpaklvnsrpleaaireffsrsqlsqfkdetnplsslrhkrrisalgpggltreragfd
vrdvhrthygricpvetpeganiglitslaayarvdelgfirtpyrrvvggvvtdevvym
tateedrytiaqantplegnriaaervvarrkgepvivspeevefmdvspkqvfsvntnl
ipflehddanralmgsnmqtqavpliraqapvvmtgleervvrdslaalyaeedgevakv
dgnrivvryedgrlveyplrrfyrsnqgtaldqrprvvvgqrvrkgdlladgpasengfl
algqnvlvaimpfdgynfedaiviseellkrdfytsihieryeieardtklgperitrdi
phlseaalrdldeegvvrigaevkpgdilvgrtsfkgeseptpeerllrsifgekardvk
dtslrvppgeggivvrtvrlrrgdpgvelkpgvrevvrvyvaqkrklqvgdklanrhgnk
gvvakilpvedmphlpdgtpvdvilnplgvpsrmnlgqilethlglagyflgqryispif
dgakepeikellaqafevyfgkrkgegfgvdkrevevlrraeklglvtpgktpeeqlkel
flqgkvvlydgrtgepiegpivvgqmfimklyhmvedkmharstgpyslitqqplggkaq
fggqrfgemevwaleaygaahtlqemltlksddiegrnaayeaiikgedvpepsvpesfr
vlvkelqalaldvqtldekdnpvdifeglask

  • Chain 'D':
    Sequence, based on SEQRES records:
    >4oipD (D:)
mkkevrkvrialaspekirswsygevekpetinyrtlkperdglfderifgpikdyecac
gkykrqrfegkvcercgvevtksivrryrmghielatpaahiwfvkdvpskigtlldlsa
teleqvlyfskyivldpkgailngvpvekrqlltdeeyrelrygkqetyplppgvdalvk
dgeevvkgqelapgvvsrldgvalyrfprrvrveyvkkeraglrlplaawvekeaykpge
ilaelpepylfraeeegvvelkeleegaflvlrredepvatyflpvgmtplvvhgeivek
gqplaeakgllrmprqvraaqveaeeegetvyltlflewtepkdyrvqphmnvvvpegar
veagdkivaaidpeeeviaeaegvvhlhepasilvvkarvypfeddvevstgdrvapgdv
ladggkvksdvygrvevdlvrnvvrvvesydidarmgaeaiqqllkeldlealekellee
mkhpsrarrakarkrlevvrafldsgnrpewmileavpvlppdlrpmvqvdggrfatsdl
ndlyrrlinrnnrlkkllaqgapeiiirnekrmlqeavdalldngrrgapvtnpgsdrpl
rsltdilsgkqgrfrqnllgkrvdysgrsvivvgpqlklhqcglpkrmalelfkpfllkk
meekgiapnvkaarrmlerqrdikdevwdaleevihgkvvllnraptlhrlgiqafqpvl
vegqsiqlhplvceafnadfdgdqmavhvplssfaqaeariqmlsahnllspasgeplak
psrdiilglyyitqvrkekkgaglefatpeealaahergevalnapikvagretsvgrlk
yvfanpdeallavahgivdlqdvvtvrymgkrletspgrilfarivaeavedekvaweli
qldvpqeknslkdlvyqaflrlgmektarlldalkyygftfsttsgitigiddavipeek
kqyleeadrkllqieqayemgfltdrerydqilqlwtettekvtqavfknfeenypfnpl
yvmaqsgargnpqqirqlcglrglmqkpsgetfevpvrssfregltvleyfisshgarkg
gadtalrtadsgyltrklvdvtheivvreadcgttnyisvplfqpdevtrslrlrkradi
eaglygrvlarevevlgvrleegrylsmddvhllikaaeageiqevpvrspltcqtrygv
cqkcygydlsmarpvsigeavgivaaqsigepgtqltmrtfhtggvagaaditqglprvi
elfearrpkakaviseidgvvrieeteeklsvfvesegfskeyklpkearllvkdgdyve
agqpltrgaidphqlleakgpeaverylveeiqkvyraqgvklhdkhieivvrqmmkyve
vtdpgdsrllegqvlekwdvealnerliaegktpvawkpllmgvtksalstkswlsaasf
qntthvlteaaiagkkdeliglkenvilgrlipagtgsdfvrftqvvdqktlkaieeark
eaveakerpaarrgvkreqpgkqa

    Sequence, based on observed residues (ATOM records):
    >4oipD (D:)
kevrkvrialaspekirswsygevekpetinyrtlkperdglfderifgpikdyecacgk
ykrqrfegkvcercgvevtksivrryrmghielatpaahiwfvkdvpskigtlldlsate
leqvlyfskyivldpkgailngvpvekrqlltdeeyrelrygkqetyplppgvdalvkdg
eevvkgqelapgvvsrldgvalyrfprrvrveyvkkeraglrlplaawvekeaykpgeil
aelpepylfraeeegvvelkeleegaflvlrredepvatyflpvgmtplvvhgeivekgq
plaeakgllrmprqvraaqveaeeegetvyltlflewtepkdyrvqphmnvvvpegarve
agdkivaaidpeeeviaeaegvvhlhepasilvvkarvypfeddvevstgdrvapgdvla
dggkvksdvygrvevdlvrnvvrvvesydidarmgaeaiqqllkeldlealekelleemk
hpsrarrakarkrlevvrafldsgnrpewmileavpvlppdlrpmvqvdggrfatsdlnd
lyrrlinrnnrlkkllaqgapeiiirnekrmlqeavdalldngrrgapvtnpgsdrplrs
ltdilsgkqgrfrqnllgkrvdysgrsvivvgpqlklhqcglpkrmalelfkpfllkkme
ekgiapnvkaarrmlerqrdikdevwdaleevihgkvvllnraptlhrlgiqafqpvlve
gqsiqlhplvceafnadfdgdqmavhvplssfaqaeariqmlsahnllspasgeplakps
rdiilglyyitqvrkekkgaglefatpeealaahergevalnapikvagretsvgrlkyv
fanpdeallavahgivdlqdvvtvrymgkrletspgrilfarivaeavedekvaweliql
dvpqeknslkdlvyqaflrlgmektarlldalkyygftfsttsgitigiddavipeekkq
yleeadrkllqieqayemgfltdrerydqilqlwtettekvtqavfknfeenypfnplyv
maqsgargnpqqirqlcglrglmqkpsgetfevpvrssfregltvleyfisshgarkgga
dtalrtadsgyltrklvdvtheivvreadcgttnyisvplfqpdevtrslrlrkradiea
glygrvlarevevlgvrleegrylsmddvhllikaaeageiqevpvrspltcqtrygvcq
kcygydlsmarpvsigeavgivaaqsigepgtqltitqglprvielfearrpkakavise
idgvvrieeteeklsvfvesegfskeyklpkearllvkdgdyveagqpltrgaidphqll
eakgpeaverylveeiqkvyraqgvklhdkhieivvrqmmkyvevtdpgdsrllegqvle
kwdvealnerliaegktpvawkpllmgvtksalstkswlsaasfqntthvlteaaiagkk
deliglkenvilgrlipagtgsdfvrftqvvdqktlkaieearkea

  • Chain 'E':
    Sequence, based on SEQRES records:
    >4oipE (E:)
maepgidklfgmvdskyrltvvvakraqqllrhgfkntvlepeerpkmqtleglfddpna
vtwamkelltgrlvfgenlvpedrlqkemerlypveree

    Sequence, based on observed residues (ATOM records):
    >4oipE (E:)
aepgidklfgmvdskyrltvvvakraqqllrhgfkntvlepeerpkmqtleglfddpnav
twamkelltgrlvfgenlvpedrlqkemerlypv

  • Chain 'F':
    Sequence, based on SEQRES records:
    >4oipF (F:)
mgsshhhhhhssglvprgshmkkskrknaqaqeaqetevlvqeeaeelpefpegepdpdl
edpdltleddlldlpeegegldleeeeedlpipkistsdpvrqylheigqvplltleeev
elarkveegmeaikklseitgldpdlirevvrakilgsarvrhipglketldpktveeid
qklkslpkehkrylhiaregeaarqhlieanlrlvvsiakkytgrglsfldliqegnqgl
iravekfeykrrfkfstyatwwirqainraiadqartiripvhmvetinklsrtarqlqq
elgreptyeeiaeamgpgwdakrveetlkiaqepvsletpigdekdsfygdfipdehlps
pvdaatqsllseelekalsklsereamvlklrkglidgrehtleevgaffgvtrerirqi
enkalrklkyhesrtrklrdfld

    Sequence, based on observed residues (ATOM records):
    >4oipF (F:)
sdpvrqylheigqvplltleeevelarkveegmeaikklseitgldpdlirevvrakilg
sarvrhipglketldpktveeidqklkslpkehkrylhiaregeaarqhlieanlrlvvs
iakkytgrglsfldliqegnqgliravekfeykrrfkfstyatwwirqainraiadqart
iripvhmvetinklsrtarqlqqelgreptyeeiaeamgpgwdakrveetlkiaqepvsl
etpigdekdsfygdfipdehlpspvdaatqsllseelekalsklsereamvlklrkglid
grehtleevgaffgvtrerirqienkalrklkyhesrtrklrdfld

  • Chain 'G':
    No sequence available.

  • Chain 'H':
    No sequence available.

  • Chain 'I':
    Sequence; same for both SEQRES and ATOM records:
    >4oipI (I:)
xsvxxxg