SCOPe 2.05: Structural Classification of Proteins

PDB entry 4niy

View 4niy on RCSB PDB site
Description: Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) complexed to YRH-ecotin (M84Y/M85R/A86H ecotin)
Class: hydrolase/hydrolase inhibitor
Keywords: Trypsin inhibitor, Serine proteinase, Enzyme design, Activation domain, Peptide ligation, Reverse proteolysis, HYDROLASE-HYDROLASE INHIBITOR complex
Deposited on 2013-11-08, released 2014-02-19
The last revision prior to the SCOPe 2.05 freeze date was dated 2014-03-19, with a file datestamp of 2014-03-14.
Experiment type: XRAY
Resolution: 2.84 Å
R-factor: 0.195
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

Chain 'A':
Compound: cationic trypsin
Species: Bos taurus [TaxId:9913]
Database cross-references and differences (RAF-indexed):
- Uniprot P00760 (0-222)
  - engineered mutation (42)
  - engineered mutation (122)
  - engineered mutation (130)
  - engineered mutation (170)
Domains in SCOPe 2.05: d4niya_
Chain 'B':
Compound: cationic trypsin
Species: Bos taurus [TaxId:9913]
Database cross-references and differences (RAF-indexed):
- Uniprot P00760 (0-222)
  - engineered mutation (42)
  - engineered mutation (122)
  - engineered mutation (130)
  - engineered mutation (170)
Domains in SCOPe 2.05: d4niyb_
Chain 'C':
Compound: cationic trypsin
Species: Bos taurus [TaxId:9913]
Database cross-references and differences (RAF-indexed):
- Uniprot P00760 (0-222)
  - engineered mutation (42)
  - engineered mutation (122)
  - engineered mutation (170)
Domains in SCOPe 2.05: d4niyc_
Chain 'D':
Compound: cationic trypsin
Species: Bos taurus [TaxId:9913]
Database cross-references and differences (RAF-indexed):
- Uniprot P00760 (Start-222)
  - engineered mutation (42)
Domains in SCOPe 2.05: d4niyd_
Chain 'E':
Compound: ecotin
Species: Escherichia coli [TaxId:83333]
Gene: eco, eti, b2209, JW2197
Database cross-references and differences (RAF-indexed):
- Uniprot P23827 (Start-141)
  - engineered mutation (83-85)
Domains in SCOPe 2.05: d4niye_
Chain 'F':
Compound: ecotin
Species: Escherichia coli [TaxId:83333]
Gene: eco, eti, b2209, JW2197
Database cross-references and differences (RAF-indexed):
- Uniprot P23827 (Start-141)
  - engineered mutation (83-85)
Domains in SCOPe 2.05: d4niyf_
Chain 'G':
Compound: ecotin
Species: Escherichia coli [TaxId:83333]
Gene: eco, eti, b2209, JW2197
Database cross-references and differences (RAF-indexed):
- Uniprot P23827 (Start-141)
  - engineered mutation (83-85)
Domains in SCOPe 2.05: d4niyg_
Chain 'H':
Compound: ecotin
Species: Escherichia coli [TaxId:83333]
Gene: eco, eti, b2209, JW2197
Database cross-references and differences (RAF-indexed):
- Uniprot P23827 (Start-141)
  - engineered mutation (83-85)
Domains in SCOPe 2.05: d4niyh_
Heterogens: CA, ZN, HOH

PDB Chain Sequences:

Chain 'A':
Sequence, based on SEQRES records: (download)

>4niyA (A:)
ivggytcgantvpyqvslnsgyhfcggslinsqwvvsaahcyesgiqvrlgedninvveg
neqfisasksivhpsynsntlnndimliklksaaslnsrvasislptscasagtqclisg
wghtkssgtshpdvlkclkapilsdsscksaypgqitsnmfcagyleggkkscqgdsggp
vvcsgklqgivswgsgcaqknkpgvytkvcnyvswikqtiasn