PDB entry 4nix

View 4nix on RCSB PDB site
Description: Crystal structure of trypsiligase (K60E/N143H/Y151H/D189K trypsin) orthorhombic form, zinc-bound
Class: hydrolase
Keywords: Trypsin, Serine proteinase, Enzyme design, Activation domain, Zymogen, Peptide ligation, Reverse proteolysis, HYDROLASE
Deposited on 2013-11-08, released 2014-02-19
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-03-19, with a file datestamp of 2014-03-14.
Experiment type: XRAY
Resolution: 1.3 Å
R-factor: 0.131
AEROSPACI score: 0.71 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: cationic trypsin
    Species: Bos taurus [TaxId:9913]
    Database cross-references and differences (RAF-indexed):
    • Uniprot P00760 (Start-222)
      • engineered mutation (42)
      • engineered mutation (122)
      • engineered mutation (130)
      • engineered mutation (170)
    Domains in SCOPe 2.06: d4nixa_
  • Heterogens: CA, GOL, ZN, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >4nixA (A:)
    ivggytcgantvpyqvslnsgyhfcggslinsqwvvsaahcyesgiqvrlgedninvveg
    neqfisasksivhpsynsntlnndimliklksaaslnsrvasislptscasagtqclisg
    wghtkssgtshpdvlkclkapilsdsscksaypgqitsnmfcagyleggkkscqgdsggp
    vvcsgklqgivswgsgcaqknkpgvytkvcnyvswikqtiasn
    

    Sequence, based on observed residues (ATOM records): (download)
    >4nixA (A:)
    ggytcgantvpyqvslnsgyhfcggslinsqwvvsaahcyesgiqvrlgedninvvegne
    qfisasksivhpsynsntlnndimliklksaaslnsrvasislptscasagtqclisgwg
    htkssgtshpdvlkclkapilsdsscksaypgqitsnmfcagyleggkkscqgdsggpvv
    csgklqgivswgsgcaqknkpgvytkvcnyvswikqtiasn