PDB entry 4nih

View 4nih on RCSB PDB site
Description: Crystal structure of AlkB E136L mutant protein with cofactors bound to dsDNA containing m6A/A
Class: oxidoreductase/DNA
Keywords: DNA/RNA direct repair, jelly-roll fold, DNA/RNA demethylation repair, Fe(II), 2-KG, OXIDOREDUCTASE-DNA complex
Deposited on 2013-11-06, released 2014-04-16
The last revision prior to the SCOPe 2.07 freeze date was dated 2014-05-28, with a file datestamp of 2014-05-23.
Experiment type: XRAY
Resolution: 1.37 Å
R-factor: 0.16
AEROSPACI score: 0.63 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Alpha-ketoglutarate-dependent dioxygenase AlkB
    Species: Escherichia coli [TaxId:562]
    Gene: alkB, aidD, b2212, JW2200
    Database cross-references and differences (RAF-indexed):
    • Uniprot P05050
      • engineered mutation (117)
      • engineered mutation (124)
    Domains in SCOPe 2.07: d4niha_
  • Chain 'B':
    Compound: 5'-d(*tp*ap*gp*gp*tp*ap*ap*(6ma)p*ap*cp*cp*gp*t)-3'
  • Chain 'C':
    Compound: 5'-d(*ap*ap*cp*gp*gp*tp*ap*tp*tp*ap*cp*cp*t)-3'
  • Heterogens: MN, AKG, GOL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4nihA (A:)
qeplaagavilrrfafnaaeqlirdindvasqspfrqmvtpggytmsvamtncghlgwtt
hrqgylyspidpqtnkpwpampqsfhnlcqraataagypdfqpdaclinryapgaklclh
qdkdlpdlrapivsvslglpaifqfgglkrndplkrlllehgdvvvwggesrlfyhgiqp
lkagfhpltidcrynltfrqagkke

    Sequence, based on observed residues (ATOM records): (download) 
    >4nihA (A:)
plaagavilrrfafnaaeqlirdindvasqspfrqmvtpggytmsvamtncghlgwtthr
qgylyspidpqtnkpwpampqsfhnlcqraataagypdfqpdaclinryapgaklclhqd
kdlpdlrapivsvslglpaifqfgglkrndplkrlllehgdvvvwggesrlfyhgiqplk
agfhpltidcrynltfrqagk

  • Chain 'B':
    No sequence available.

  • Chain 'C':
    No sequence available.