PDB entry 4mne

View 4mne on RCSB PDB site
Description: Crystal structure of the BRAF:MEK1 complex
Class: transferase/transferase inhibitor
Keywords: RAF, MAPK, kinase domain, ATP-binding, ERK, RAS, PAK, TRANSFERASE-TRANSFERASE INHIBITOR complex
Deposited on 2013-09-10, released 2014-06-18
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-09-24, with a file datestamp of 2014-09-19.
Experiment type: XRAY
Resolution: 2.85 Å
R-factor: 0.186
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Dual specificity mitogen-activated protein kinase kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: MAP2K1, MEK1, PRKMK1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mnea_
  • Chain 'B':
    Compound: Serine/threonine-protein kinase B-raf
    Species: Homo sapiens [TaxId:9606]
    Gene: BRAF, BRAF1, RAFB1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mneb_
  • Chain 'C':
    Compound: Serine/threonine-protein kinase B-raf
    Species: Homo sapiens [TaxId:9606]
    Gene: BRAF, BRAF1, RAFB1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mnec_
  • Chain 'D':
    Compound: Dual specificity mitogen-activated protein kinase kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: MAP2K1, MEK1, PRKMK1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mned_
  • Chain 'E':
    Compound: Dual specificity mitogen-activated protein kinase kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: MAP2K1, MEK1, PRKMK1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mnee_
  • Chain 'F':
    Compound: Serine/threonine-protein kinase B-raf
    Species: Homo sapiens [TaxId:9606]
    Gene: BRAF, BRAF1, RAFB1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mnef_
  • Chain 'G':
    Compound: Serine/threonine-protein kinase B-raf
    Species: Homo sapiens [TaxId:9606]
    Gene: BRAF, BRAF1, RAFB1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mneg_
  • Chain 'H':
    Compound: Dual specificity mitogen-activated protein kinase kinase 1
    Species: Homo sapiens [TaxId:9606]
    Gene: MAP2K1, MEK1, PRKMK1
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.08: d4mneh_
  • Heterogens: ACP, MG, 573, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4mneA (A:)
melkdddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhe
cnspyivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltyl
rekhkimhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthy
svqsdiwsmglslvemavgrypipppdakelelmfgcqvegdaaetpprprtpgrplssy
gmdsrppmaifelldyivnepppklpsgvfslefqdfvnkcliknpaeradlkqlmvhaf
ikrsdaeevdfagwlcstiglnqpstpthaagvlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneA (A:)
lkdddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhecn
spyivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltylre
khkimhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthysv
qsdiwsmglslvemavgrypipppdakelelmppmaifelldyivnepppklpsgvfsle
fqdfvnkcliknpaeradlkqlmvhafikrsdaeevdfagwlcsti

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4mneB (B:)
mdrgshhhhhhgsedrnrmktlgrrdssddweipdgqitvgqrigsgsfgtvykgkwhgd
vavkmlnvtaptpqqlqafknevgvlrktrhvnillfmgystkpqlaivtqwcegsslyh
hlhiietkfemiklidiarqtaqgmdylhaksiihrdlksnniflhedltvkigdfglat
vksrwsgshqfeqlsgsilwmapevirmqdknpysfqsdvyafgivlyelmtgqlpysni
nnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkkrderplfpqilasiellar
slpkihrk

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneB (B:)
dweipdgqitvgqriggtvykgkwhgdvavkmlnvtaptpqqlqafknevgvlrktrhvn
illfmgystkpqlaivtqwcegsslyhhlhiietkfemiklidiarqtaqgmdylhaksi
ihrdlksnniflhedltvkigdfglatvksrwsgshqfeqlsgsilwmapevirmqdknp
ysfqsdvyafgivlyelmtgqlpysninnrdqiifmvgrgylspdlskvrsncpkamkrl
maeclkkkrderplfpqilasiellarslp

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4mneC (C:)
mdrgshhhhhhgsedrnrmktlgrrdssddweipdgqitvgqrigsgsfgtvykgkwhgd
vavkmlnvtaptpqqlqafknevgvlrktrhvnillfmgystkpqlaivtqwcegsslyh
hlhiietkfemiklidiarqtaqgmdylhaksiihrdlksnniflhedltvkigdfglat
vksrwsgshqfeqlsgsilwmapevirmqdknpysfqsdvyafgivlyelmtgqlpysni
nnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkkrderplfpqilasiellar
slpkihrk

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneC (C:)
dweipdgqitvgqrigtvykgkwhgdvavkmlnvtaptpqqlqafknevgvlrktrhvni
llfmgystkpqlaivtqwcegsslyhhlhiietkfemiklidiarqtaqgmdylhaksii
hrdlksnniflhedltvkigdfglatvksfeqlsgsilwmapevirmqdknpysfqsdvy
afgivlyelmtgqlpysninnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkk
rderplfpqilasiellars

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4mneD (D:)
melkdddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhe
cnspyivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltyl
rekhkimhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthy
svqsdiwsmglslvemavgrypipppdakelelmfgcqvegdaaetpprprtpgrplssy
gmdsrppmaifelldyivnepppklpsgvfslefqdfvnkcliknpaeradlkqlmvhaf
ikrsdaeevdfagwlcstiglnqpstpthaagvlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneD (D:)
ddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhecnspy
ivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltylrekhk
imhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthysvqsd
iwsmglslvemavgrypipppdakelelmpmaifelldyivnepppklpsgvfslefqdf
vnkcliknpaeradlkqlmvhafikrsdaeevdfagwlcstigln

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4mneE (E:)
melkdddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhe
cnspyivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltyl
rekhkimhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthy
svqsdiwsmglslvemavgrypipppdakelelmfgcqvegdaaetpprprtpgrplssy
gmdsrppmaifelldyivnepppklpsgvfslefqdfvnkcliknpaeradlkqlmvhaf
ikrsdaeevdfagwlcstiglnqpstpthaagvlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneE (E:)
elkdddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhec
nspyivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltylr
ekhkimhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthys
vqsdiwsmglslvemavgrypipppdakelelmpmaifelldyivnepppklpsgvfsle
fqdfvnkcliknpaeradlkqlmvhafikrsdaeevdfagwlcsti

  • Chain 'F':
    Sequence, based on SEQRES records: (download) 
    >4mneF (F:)
mdrgshhhhhhgsedrnrmktlgrrdssddweipdgqitvgqrigsgsfgtvykgkwhgd
vavkmlnvtaptpqqlqafknevgvlrktrhvnillfmgystkpqlaivtqwcegsslyh
hlhiietkfemiklidiarqtaqgmdylhaksiihrdlksnniflhedltvkigdfglat
vksrwsgshqfeqlsgsilwmapevirmqdknpysfqsdvyafgivlyelmtgqlpysni
nnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkkrderplfpqilasiellar
slpkihrk

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneF (F:)
dweipdgqitvgqritvykgkwhgdvavkmlnvtaptpqqlqafknevgvlrktrhvnil
lfmgystkpqlaivtqwcegsslyhhlhiietkfemiklidiarqtaqgmdylhaksiih
rdlksnniflhedltvkigdfglatvksqlsgsilwmapevirmqdknpysfqsdvyafg
ivlyelmtgqlpysninnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkkrde
rplfpqilasiellarsl

  • Chain 'G':
    Sequence, based on SEQRES records: (download) 
    >4mneG (G:)
mdrgshhhhhhgsedrnrmktlgrrdssddweipdgqitvgqrigsgsfgtvykgkwhgd
vavkmlnvtaptpqqlqafknevgvlrktrhvnillfmgystkpqlaivtqwcegsslyh
hlhiietkfemiklidiarqtaqgmdylhaksiihrdlksnniflhedltvkigdfglat
vksrwsgshqfeqlsgsilwmapevirmqdknpysfqsdvyafgivlyelmtgqlpysni
nnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkkrderplfpqilasiellar
slpkihrk

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneG (G:)
dweipdgqitvgqrigtvykgkwhgdvavkmlnvtaptpqqlqafknevgvlrktrhvni
llfmgystkpqlaivtqwcegsslyhhlhiietkfemiklidiarqtaqgmdylhaksii
hrdlksnniflhedltvkigdfglatvkseqlsgsilwmapevirmqdknpysfqsdvya
fgivlyelmtgqlpysninnrdqiifmvgrgylspdlskvrsncpkamkrlmaeclkkkr
derplfpqilasiellars

  • Chain 'H':
    Sequence, based on SEQRES records: (download) 
    >4mneH (H:)
melkdddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhe
cnspyivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltyl
rekhkimhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthy
svqsdiwsmglslvemavgrypipppdakelelmfgcqvegdaaetpprprtpgrplssy
gmdsrppmaifelldyivnepppklpsgvfslefqdfvnkcliknpaeradlkqlmvhaf
ikrsdaeevdfagwlcstiglnqpstpthaagvlehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4mneH (H:)
ddfekiselgagnggvvfkvshkpsglvmarklihleikpairnqiirelqvlhecnspy
ivgfygafysdgeisicmehmdggsldqvlkkagripeqilgkvsiavikgltylrekhk
imhrdvkpsnilvnsrgeiklcdfgvsgqlidsmansfvgtrsymsperlqgthysvqsd
iwsmglslvemavgrypippppmaifelldyivnepppklpsgvfslefqdfvnkclikn
paeradlkqlmvhafikrsdaeevdfagwlcstig