PDB entry 4mih

View 4mih on RCSB PDB site
Description: pyranose 2-oxidase from phanerochaete chrysosporium, recombinant h158a mutant
Deposited on 2013-08-31, released 2013-12-11
The last revision was dated 2020-07-29, with a file datestamp of 2020-07-04.
Experiment type: XRAY
Resolution: 2.4 Å
R-factor: N/A
AEROSPACI score: 0.29 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'B':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'C':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'D':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'E':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'F':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'G':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Chain 'H':
    Compound: Pyranose 2-oxidase
    Species: Phanerochaete chrysosporium [TaxId:5306]
    Gene: p2o, p2ox, pox
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6QWR1 (1-End)
      • engineered mutation (158)
  • Heterogens: FAD, G3F, HOH

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >4mihA (A:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihA (A:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshippdatiptplmpmlgkyiteqpmtfcqvvldsslme
vvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrdafs
ygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrarar
rmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnl
yvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'B':
    Sequence, based on SEQRES records:
    >4mihB (B:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihB (B:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshipperdatiptplmpmlgkyiteqpmtfcqvvldssl
mevvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrda
fsygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrar
arrmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfn
nlyvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'C':
    Sequence, based on SEQRES records:
    >4mihC (C:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihC (C:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshipperdatiptplmpmlgkyiteqpmtfcqvvldssl
mevvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrda
fsygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrar
arrmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfn
nlyvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'D':
    Sequence, based on SEQRES records:
    >4mihD (D:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihD (D:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshipperdatiptplmpmlgkyiteqpmtfcqvvldssl
mevvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrda
fsygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrar
arrmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfn
nlyvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'E':
    Sequence, based on SEQRES records:
    >4mihE (E:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihE (E:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshippdatiptplmpmlgkyiteqpmtfcqvvldsslme
vvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrdafs
ygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrarar
rmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnl
yvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'F':
    Sequence, based on SEQRES records:
    >4mihF (F:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihF (F:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshipperdatiptplmpmlgkyiteqpmtfcqvvldssl
mevvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrda
fsygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrar
arrmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfn
nlyvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'G':
    Sequence, based on SEQRES records:
    >4mihG (G:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihG (G:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshipperdatiptplmpmlgkyiteqpmtfcqvvldssl
mevvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrda
fsygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrar
arrmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfn
nlyvggngvietgfaanptltsicyairasndiiakfg

  • Chain 'H':
    Sequence, based on SEQRES records:
    >4mihH (H:)
smfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgdp
naprsvqfgpgqvpipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsv
vsnsldkpfislgknpaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrer
pklstdaaedariwkdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsp
lplachrltdpdyvewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenev
dyalvedllphmqnpgnpasvkkiyarsyvvacgavataqvlanshippddvvipfpgge
kgsgggerdatiptplmpmlgkyiteqpmtfcqvvldsslmevvrnppwpgldwwkekva
rhveafpndpipipfrdpepqvtikfteehpwhvqihrdafsygavaenmdtrvivdyrf
fgytepqeanelvfqqhyrdaydmpqptfkftmsqddrararrmmddmcnialkiggylp
gsepqfmtpglalhlagttrcgldtqktvgnthckvhnfnnlyvggngvietgfaanptl
tsicyairasndiiakfgrhr

    Sequence, based on observed residues (ATOM records):
    >4mihH (H:)
mfldttpfradepydvfiagsgpigatfaklcvdanlrvcmveigaadsftskpmkgqvp
ipgyhkkneieyqkdidrfvnvikgalstcsiptsnnhiatldpsvvsnsldkpfislgk
npaqnpfvnlgaeavtrgvggmstawtcatpeffapadfnaphrerpklstdaaedariw
kdlyaqakeiigtsttefdhsirhnlvlrkyndifqkenvirefsplplachrltdpdyv
ewhatdrileelftdpvkrgrftlltnhrctklvfkhyrpgeenevdyalvedllpsvkk
iyarsyvvacgavataqvlanshipperdatiptplmpmlgkyiteqpmtfcqvvldssl
mevvrnppwpgldwwkekvarhveafpndpipipfrdpepqvtikfteehpwhvqihrda
fsygavaenmdtrvivdyrffgytepqeanelvfqqhyrdaydmpqptfkftmsqddrar
arrmmddmcnialkiggylpgsepqfmtpglalhlagttrcgldtqktvgnthckvhnfn
nlyvggngvietgfaanptltsicyairasndiiakfg