PDB entry 4lqu

View 4lqu on RCSB PDB site
Description: 1.60A resolution crystal structure of a superfolder green fluorescent protein (W57G) mutant
Class: fluorescent protein
Keywords: GFP, Chemical biology, allosteric activation, switchable enzyme, chemical rescue, mutation, FLUORESCENT PROTEIN
Deposited on 2013-07-19, released 2013-12-18
The last revision prior to the SCOPe 2.05 freeze date was dated 2013-12-18, with a file datestamp of 2013-12-13.
Experiment type: XRAY
Resolution: 1.6 Å
R-factor: 0.156
AEROSPACI score: 0.53 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (11-End)
      • expression tag (10)
      • engineered mutation (39)
      • engineered mutation (48)
      • engineered mutation (66)
      • chromophore (75)
      • engineered mutation (108)
      • engineered mutation (114)
      • engineered mutation (154)
      • engineered mutation (162)
      • engineered mutation (172)
      • engineered mutation (180)
    Domains in SCOPe 2.05: d4lqua_
  • Chain 'B':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (11-End)
      • expression tag (10)
      • engineered mutation (39)
      • engineered mutation (48)
      • engineered mutation (66)
      • chromophore (75)
      • engineered mutation (108)
      • engineered mutation (114)
      • engineered mutation (154)
      • engineered mutation (162)
      • engineered mutation (172)
      • engineered mutation (180)
    Domains in SCOPe 2.05: d4lqub_
  • Chain 'C':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (11-End)
      • expression tag (10)
      • engineered mutation (39)
      • engineered mutation (48)
      • engineered mutation (66)
      • chromophore (75)
      • engineered mutation (108)
      • engineered mutation (114)
      • engineered mutation (154)
      • engineered mutation (162)
      • engineered mutation (172)
      • engineered mutation (180)
    Domains in SCOPe 2.05: d4lquc_
  • Chain 'D':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (11-End)
      • expression tag (10)
      • engineered mutation (39)
      • engineered mutation (48)
      • engineered mutation (66)
      • chromophore (75)
      • engineered mutation (108)
      • engineered mutation (114)
      • engineered mutation (154)
      • engineered mutation (162)
      • engineered mutation (172)
      • engineered mutation (180)
    Domains in SCOPe 2.05: d4lqud_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4lquA (A:)
mghhhhhhggaskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkfictt
gklpvpgptlvttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktra
evkfegdtlvnrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhn
vedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit
hgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >4lquA (A:)
askgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpgptl
vttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktraevkfegdtlv
nrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqlad
hyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaag

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4lquB (B:)
mghhhhhhggaskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkfictt
gklpvpgptlvttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktra
evkfegdtlvnrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhn
vedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit
hgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >4lquB (B:)
askgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpgptl
vttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktraevkfegdtlv
nrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqlad
hyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagith

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4lquC (C:)
mghhhhhhggaskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkfictt
gklpvpgptlvttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktra
evkfegdtlvnrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhn
vedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit
hgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >4lquC (C:)
askgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpgptl
vttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktraevkfegdtlv
nrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqlad
hyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4lquD (D:)
mghhhhhhggaskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkfictt
gklpvpgptlvttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktra
evkfegdtlvnrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhn
vedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit
hgmdelyk

    Sequence, based on observed residues (ATOM records): (download) 
    >4lquD (D:)
askgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpgptl
vttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktraevkfegdtlv
nrielkgidfkedgnilghkleynfnshnvyitadqkngikanfkirhnvedgsvqladh
yqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit