PDB entry 4lqt

View 4lqt on RCSB PDB site
Description: 1.10A resolution crystal structure of a superfolder green fluorescent protein (W57A) mutant
Class: fluorescent protein
Keywords: GFP, Chemical biology, allosteric activation, switchable enzyme, chemical rescue, mutation, FLUORESCENT PROTEIN
Deposited on 2013-07-19, released 2013-12-18
The last revision prior to the SCOPe 2.06 freeze date was dated 2014-12-31, with a file datestamp of 2014-12-26.
Experiment type: XRAY
Resolution: 1.1 Å
R-factor: 0.135
AEROSPACI score: 0.84 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (11-End)
      • expression tag (10)
      • engineered mutation (39)
      • engineered mutation (48)
      • engineered mutation (66)
      • chromophore (75)
      • engineered mutation (108)
      • engineered mutation (114)
      • engineered mutation (154)
      • engineered mutation (162)
      • engineered mutation (172)
      • engineered mutation (180)
    Domains in SCOPe 2.06: d4lqta1, d4lqta2
  • Heterogens: EDO, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download)
    >4lqtA (A:)
    mghhhhhhggaskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkfictt
    gklpvpaptlvttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktra
    evkfegdtlvnrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhn
    vedgsvqladhyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagit
    hgmdelyk
    

    Sequence, based on observed residues (ATOM records): (download)
    >4lqtA (A:)
    askgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpaptl
    vttlgygvqcfsrypdhmkqhdffksampegyvqertisfkddgtyktraevkfegdtlv
    nrielkgidfkedgnilghkleynfnshnvyitadkqkngikanfkirhnvedgsvqlad
    hyqqntpigdgpvllpdnhylstqsalskdpnekrdhmvllefvtaagithg