PDB entry 4l8f

View 4l8f on RCSB PDB site
Description: Crystal structure of gamma-glutamyl hydrolase (C108A) complex with MTX
Class: Hydrolase
Keywords: sandwiched-like domains, Gamma-glutamyl Hydrolase, Hydrolase
Deposited on 2013-06-17, released 2014-05-14
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-05-14, with a file datestamp of 2014-05-09.
Experiment type: XRAY
Resolution: 1.97 Å
R-factor: 0.178
AEROSPACI score: 0.39 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (128)
    Domains in SCOPe 2.08: d4l8fa_
  • Chain 'B':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (128)
    Domains in SCOPe 2.08: d4l8fb_
  • Chain 'C':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (128)
    Domains in SCOPe 2.08: d4l8fc_
  • Chain 'D':
    Compound: gamma-glutamyl hydrolase
    Species: Danio rerio [TaxId:7955]
    Gene: ggh
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q6NY42 (Start-311)
      • engineered mutation (128)
    Domains in SCOPe 2.08: d4l8fd_
  • Heterogens: MTX, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4l8fA (A:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtalgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwhp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8fA (A:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtalgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwhpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4l8fB (B:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtalgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwhp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8fB (B:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtalgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwhpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4l8fC (C:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtalgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwhp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8fC (C:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtalgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwhpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4l8fD (D:)
mihifllclftvanavsiynfgpliktnerpiigvlaqdvfdpkpdrnsyiaasyvkfle
sagarvvpvminksedeysrlfksingvlfpgggvslessgyskaagifyrlaleansng
dyfpvwgtalgfelltlltsgelllshtntsgialpldftedvkgsrlfkefpeelmksl
atepltenshqwsittenftankklkkfyrvlstntdgynkfvstmeaydfpiyatqwhp
eknafewtrpyiphtpsaikttfymanffvnearknlhsfasteeeekaliynykpeytg
iqsafeqtyffn

    Sequence, based on observed residues (ATOM records): (download) 
    >4l8fD (D:)
ktnerpiigvlaqdvfdpkpdrnsyiaasyvkflesagarvvpvminksedeysrlfksi
ngvlfpgggvslessgyskaagifyrlaleansngdyfpvwgtalgfelltlltsgelll
shtntsgialpldftedvkgsrlfkefpeelmkslatepltenshqwsittenftankkl
kkfyrvlstntdgynkfvstmeaydfpiyatqwhpeknafewtrpyiphtpsaikttfym
anffvnearknlhsfasteeeekaliynykpeytgiqsafeqtyffn