PDB entry 4l27

View 4l27 on RCSB PDB site
Description: Crystal structure of delta1-39 and delta516-525 human cystathionine beta-synthase D444N mutant containing C-terminal 6xHis tag
Deposited on 2013-06-04, released 2013-09-18
The last revision was dated 2013-10-16, with a file datestamp of 2013-10-11.
Experiment type: XRAY
Resolution: 3.39 Å
R-factor: 0.207
AEROSPACI score: 0.21 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: cystathionine beta-synthase
    Species: Homo sapiens [TaxId:9606]
    Gene: CBS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P35520
      • engineered mutation (442)
  • Chain 'B':
    Compound: cystathionine beta-synthase
    Species: Homo sapiens [TaxId:9606]
    Gene: CBS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P35520
      • engineered mutation (442)
  • Chain 'C':
    Compound: cystathionine beta-synthase
    Species: Homo sapiens [TaxId:9606]
    Gene: CBS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P35520
      • engineered mutation (442)
  • Chain 'D':
    Compound: cystathionine beta-synthase
    Species: Homo sapiens [TaxId:9606]
    Gene: CBS
    Database cross-references and differences (RAF-indexed):
    • Uniprot P35520
      • engineered mutation (442)
  • Heterogens: PLP, HEM

PDB Chain Sequences:
This PDB entry is not classified in SCOPe 2.08, so the chain sequences below are not included in the ASTRAL sequence sets.

  • Chain 'A':
    Sequence, based on SEQRES records:
    >4l27A (A:)
psetpqaevgptgcphrsgphsakgslekgspedkeakeplwirpdapsrctwqlgrpas
esphhhtapakspkilpdilkkigdtpmvrinkigkkfglkcellakceffnaggsvkdr
islrmiedaerdgtlkpgdtiieptsgntgiglalaaavrgyrciivmpekmssekvdvl
ralgaeivrtptnarfdspeshvgvawrlkneipnshildqyrnasnplahydttadeil
qqcdgkldmlvasvgtggtitgiarklkekcpgcriigvdpegsilaepeelnqteqtty
evegigydfiptvldrtvvdkwfksndeeaftfarmliaqegllcggsagstvavavkaa
qelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeedltekkpwwwhlrvqelglsa
pltvlptitcghtieilrekgfnqapvvdeagvilgmvtlgnmlssllagkvqpsdqvgk
viykqfkqirltdtlgrlshilemdhfalvvheqqrqmvfgvvtaidllnfvaaqerdqk
lehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4l27A (A:)
plwirpdapsrctwqlgrsesphhhtapakspkilpdilkkigdtpmvrinkigkkfglk
cellakceffnaggsvkdrislrmiedaerdgtlkpgdtiieptsgntgiglalaaavrg
yrciivmpekmssekvdvlralgaeivrtptnarfdspeshvgvawrlkneipnshildq
yrnasnplahydttadeilqqcdgkldmlvasvgtggtitgiarklkekcpgcriigvdp
egsilaepeelnqteqttyevegigydfiptvldrtvvdkwfksndeeaftfarmliaqe
gllcggsagstvavavkaaqelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeeek
kpwwwhlrvqelglsapltvlitcgtieilrekgfnqapvvdeilgmvtlgnmlssllag
kvqpsdvgkviykqfkqirltdtlgrlshilemdhfalvvherqmvfgvvtaidllnfva
aqerd

  • Chain 'B':
    Sequence, based on SEQRES records:
    >4l27B (B:)
psetpqaevgptgcphrsgphsakgslekgspedkeakeplwirpdapsrctwqlgrpas
esphhhtapakspkilpdilkkigdtpmvrinkigkkfglkcellakceffnaggsvkdr
islrmiedaerdgtlkpgdtiieptsgntgiglalaaavrgyrciivmpekmssekvdvl
ralgaeivrtptnarfdspeshvgvawrlkneipnshildqyrnasnplahydttadeil
qqcdgkldmlvasvgtggtitgiarklkekcpgcriigvdpegsilaepeelnqteqtty
evegigydfiptvldrtvvdkwfksndeeaftfarmliaqegllcggsagstvavavkaa
qelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeedltekkpwwwhlrvqelglsa
pltvlptitcghtieilrekgfnqapvvdeagvilgmvtlgnmlssllagkvqpsdqvgk
viykqfkqirltdtlgrlshilemdhfalvvheqqrqmvfgvvtaidllnfvaaqerdqk
lehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4l27B (B:)
lwirpdapsrctwqlgrpasesphhhtapakspkilpdilkkigdtpmvrinkigkkfgl
kcellakceffnaggsvkdrislrmiedaerdgtlkpgdtiieptsgntgiglalaaavr
gyrciivmpekmssekvdvlralgaeivrtptnarfdspeshvgvawrlkneipnshild
qyrnasnplahydttadeilqqcdgkldmlvasvgtggtitgiarklkekcpgcriigvd
pegsilaepeelnqteqttyevegigydfiptvldrtvvdkwfksndeeaftfarmliaq
egllcggsagstvavavkaaqelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeed
ekkpwwwhlrvqelglsapltvlptitcghtieilrekgfnqapvvdegvilgmvtlgnm
lssllagkvqpsdqvgkviykqfkqirltdtlgrlshilemdhfalvvhemvfgvvtaid
llnfvaaqer

  • Chain 'C':
    Sequence, based on SEQRES records:
    >4l27C (C:)
psetpqaevgptgcphrsgphsakgslekgspedkeakeplwirpdapsrctwqlgrpas
esphhhtapakspkilpdilkkigdtpmvrinkigkkfglkcellakceffnaggsvkdr
islrmiedaerdgtlkpgdtiieptsgntgiglalaaavrgyrciivmpekmssekvdvl
ralgaeivrtptnarfdspeshvgvawrlkneipnshildqyrnasnplahydttadeil
qqcdgkldmlvasvgtggtitgiarklkekcpgcriigvdpegsilaepeelnqteqtty
evegigydfiptvldrtvvdkwfksndeeaftfarmliaqegllcggsagstvavavkaa
qelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeedltekkpwwwhlrvqelglsa
pltvlptitcghtieilrekgfnqapvvdeagvilgmvtlgnmlssllagkvqpsdqvgk
viykqfkqirltdtlgrlshilemdhfalvvheqqrqmvfgvvtaidllnfvaaqerdqk
lehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4l27C (C:)
lwirpdapsrctwqlgrpasesphhhtapakspkilpdilkkigdtpmvrinkigkkfgl
kcellakceffnaggsvkdrislrmiedaerdgtlkpgdtiieptsgntgiglalaaavr
gyrciivmpekmssekvdvlralgaeivrtptnarfdspeshvgvawrlkneipnshild
qyrnasnplahydttadeilqqcdgkldmlvasvgtggtitgiarklkekcpgcriigvd
pegsilaepeelnqteqttyevegigydfiptvldrtvvdkwfksndeeaftfarmliaq
egllcggsagstvavavkaaqelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeek
kpwwwhlrvqelglsapltvlptitcghtieilrekgfnqapvvdeagvilgmvtlgnml
ssllagkvqpsdqvgkviykqfkqirltdtlgrlshilemdhfalvvherqmvfgvvtai
dllnfvaaqer

  • Chain 'D':
    Sequence, based on SEQRES records:
    >4l27D (D:)
psetpqaevgptgcphrsgphsakgslekgspedkeakeplwirpdapsrctwqlgrpas
esphhhtapakspkilpdilkkigdtpmvrinkigkkfglkcellakceffnaggsvkdr
islrmiedaerdgtlkpgdtiieptsgntgiglalaaavrgyrciivmpekmssekvdvl
ralgaeivrtptnarfdspeshvgvawrlkneipnshildqyrnasnplahydttadeil
qqcdgkldmlvasvgtggtitgiarklkekcpgcriigvdpegsilaepeelnqteqtty
evegigydfiptvldrtvvdkwfksndeeaftfarmliaqegllcggsagstvavavkaa
qelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeedltekkpwwwhlrvqelglsa
pltvlptitcghtieilrekgfnqapvvdeagvilgmvtlgnmlssllagkvqpsdqvgk
viykqfkqirltdtlgrlshilemdhfalvvheqqrqmvfgvvtaidllnfvaaqerdqk
lehhhhhh

    Sequence, based on observed residues (ATOM records):
    >4l27D (D:)
wirpdapsrctwqlgrpasesphhhtapakspkilpdilkkigdtpmvrinkigkkfglk
cellakceffnaggsvkdrislrmiedaerdgtlkpgdtiieptsgntgiglalaaavrg
yrciivmpekmssekvdvlralgaeivrtptnarfdspeshvgvawrlkneipnshildq
yrnasnplahydttadeilqqcdgkldmlvasvgtggtitgiarklkekcpgcriigvdp
egsilaepeelnqteqttyevegigydfiptvldrtvvdkwfksndeeaftfarmliaqe
gllcggsagstvavavkaaqelqegqrcvvilpdsvrnymtkflsdrwmlqkgflkeedt
ekkpwwwhlrvqelglsapltvlptitcghtieilrekgfnqapvveagvilgmvtlgnm
lssllagkvqpsdqvgkviykqfkqirltdtlgrlshilemdhfalvvheqmvfgvvtai
dllnfvaaqer