PDB entry 4knf

View 4knf on RCSB PDB site
Description: Crystal structure of a blue-light absorbing proteorhodopsin double-mutant D97N/Q105L from HOT75
Class: proton transport
Keywords: Membrane, Proton transport, ion transport, proton pump, Retinylidene Schiff base
Deposited on 2013-05-09, released 2013-06-05
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-10-23, with a file datestamp of 2013-10-18.
Experiment type: XRAY
Resolution: 2.6 Å
R-factor: 0.222
AEROSPACI score: 0.22 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Blue-light absorbing proteorhodopsin
    Species: gamma proteobacterium 'Hot 75m4' [TaxId:245185]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AFF7 (Start-250)
      • engineered mutation (97)
      • engineered mutation (105)
      • expression tag (251-252)
    Domains in SCOPe 2.08: d4knfa1, d4knfa2
  • Chain 'B':
    Compound: Blue-light absorbing proteorhodopsin
    Species: gamma proteobacterium 'Hot 75m4' [TaxId:245185]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AFF7 (Start-250)
      • engineered mutation (97)
      • engineered mutation (105)
      • expression tag (251-255)
    Domains in SCOPe 2.08: d4knfb1, d4knfb2
  • Chain 'C':
    Compound: Blue-light absorbing proteorhodopsin
    Species: gamma proteobacterium 'Hot 75m4' [TaxId:245185]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AFF7 (Start-250)
      • engineered mutation (97)
      • engineered mutation (105)
      • expression tag (251-253)
    Domains in SCOPe 2.08: d4knfc1, d4knfc2
  • Chain 'D':
    Compound: Blue-light absorbing proteorhodopsin
    Species: gamma proteobacterium 'Hot 75m4' [TaxId:245185]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AFF7 (Start-250)
      • engineered mutation (97)
      • engineered mutation (105)
      • expression tag (251-253)
    Domains in SCOPe 2.08: d4knfd1, d4knfd2
  • Chain 'E':
    Compound: Blue-light absorbing proteorhodopsin
    Species: gamma proteobacterium 'Hot 75m4' [TaxId:245185]
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9AFF7 (Start-250)
      • engineered mutation (97)
      • engineered mutation (105)
      • expression tag (251-252)
    Domains in SCOPe 2.08: d4knfe1, d4knfe2
  • Heterogens: RET, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4knfA (A:)
mgklllilgsaialpsfaaaggdldisdtvgvsfwlvtagmlaatvfffverdqvsakwk
tsltvsglitgiafwhylymrgvwidtgdtptvfryinwlltvpllvvefylilaactsv
aaslfkkllagslvmlgagfageaglapvlpafiigmagwlymiyelymgegkaavstas
pavnsaynammmiivvgwaiypagyaagylmggegvyasnlnliynladfvnkilfglii
wnvavkessnakllehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4knfA (A:)
gdldisdtvgvsfwlvtagmlaatvfffverdqvsakwktsltvsglitgiafwhylymr
gvwidtgdtptvfryinwlltvpllvvefylilaactsvaaslfkkllagslvmlgagfa
geaglapvlpafiigmagwlymiyelymgegkaavstaspavnsaynammmiivvgwaiy
pagyaagylmgggvyasnlnliynladfvnkilfgliiwnvavkessnakl

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4knfB (B:)
mgklllilgsaialpsfaaaggdldisdtvgvsfwlvtagmlaatvfffverdqvsakwk
tsltvsglitgiafwhylymrgvwidtgdtptvfryinwlltvpllvvefylilaactsv
aaslfkkllagslvmlgagfageaglapvlpafiigmagwlymiyelymgegkaavstas
pavnsaynammmiivvgwaiypagyaagylmggegvyasnlnliynladfvnkilfglii
wnvavkessnakllehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4knfB (B:)
gdldisdtvgvsfwlvtagmlaatvfffverdqvsakwktsltvsglitgiafwhylymr
gvwidtgdtptvfryinwlltvpllvvefylivaaslfkkllagslvmlgagfageagla
pvlpafiigmagwlymiyelymgegkaavsspavnsaynammmiivvgwaiypagyaagy
lmgvyasnlnliynladfvnkilfgliiwnvavkessnaklleh

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4knfC (C:)
mgklllilgsaialpsfaaaggdldisdtvgvsfwlvtagmlaatvfffverdqvsakwk
tsltvsglitgiafwhylymrgvwidtgdtptvfryinwlltvpllvvefylilaactsv
aaslfkkllagslvmlgagfageaglapvlpafiigmagwlymiyelymgegkaavstas
pavnsaynammmiivvgwaiypagyaagylmggegvyasnlnliynladfvnkilfglii
wnvavkessnakllehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4knfC (C:)
gdldisdtvgvsfwlvtagmlaatvfffverdqvsakwktsltvsglitgiafwhylymr
gvwidtgdtptvfryinwlltvpllvvefylilaactsvaaslfkkllagslvmlgagfa
geaglapvlpafiigmagwlymiyelymgegkaavstaspavnsaynammmiivvgwaiy
pagyaagylmgggvyasnlnliynladfvnkilfgliiwnvavkessnakll

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4knfD (D:)
mgklllilgsaialpsfaaaggdldisdtvgvsfwlvtagmlaatvfffverdqvsakwk
tsltvsglitgiafwhylymrgvwidtgdtptvfryinwlltvpllvvefylilaactsv
aaslfkkllagslvmlgagfageaglapvlpafiigmagwlymiyelymgegkaavstas
pavnsaynammmiivvgwaiypagyaagylmggegvyasnlnliynladfvnkilfglii
wnvavkessnakllehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4knfD (D:)
ggdldisdtvgvsfwlvtagmlaatvfffverdqvsakwktsltvsglitgiafwhylym
rgvwidtgdtptvfryinwlltvpllvvefylilaactsvaaslfkkllagslvmlgagf
ageaglapvlpafiigmagwlymiyelymgegkaaaspavnsaynammmiivvgwaiypa
gyaagylmggvyasnlnliynladfvnkilfgliiwnvavkessnakll

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4knfE (E:)
mgklllilgsaialpsfaaaggdldisdtvgvsfwlvtagmlaatvfffverdqvsakwk
tsltvsglitgiafwhylymrgvwidtgdtptvfryinwlltvpllvvefylilaactsv
aaslfkkllagslvmlgagfageaglapvlpafiigmagwlymiyelymgegkaavstas
pavnsaynammmiivvgwaiypagyaagylmggegvyasnlnliynladfvnkilfglii
wnvavkessnakllehhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4knfE (E:)
gdldisdtvgvsfwlvtagmlaatvfffverdqvsakwktsltvsglitgiafwhylymr
gvwidtgdtptvfryinwlltvpllvvefylilaactsvaaslfkkllagslvmlgagfa
geaglapvlpafiigmagwlymiyelymgegkaavstaspavnsaynammmiivvgwaiy
pagyaagylmggvyasnlnliynladfvnkilfgliiwnvavkessnakl