PDB entry 4kk8

View 4kk8 on RCSB PDB site
Description: Structure of the E148Q mutant of CLC-ec1 deltaNC construct in 100mM fluoride
Class: transport protein
Keywords: membrane transporter, TRANSPORT PROTEIN
Deposited on 2013-05-05, released 2013-08-21
The last revision prior to the SCOPe 2.04 freeze date was dated 2013-11-13, with a file datestamp of 2013-11-08.
Experiment type: XRAY
Resolution: 2.86 Å
R-factor: 0.212
AEROSPACI score: 0.19 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: H(+)/Cl(-) exchange transporter clcA
    Species: Escherichia coli [TaxId:83333]
    Gene: b0155, clcA, eriC, JW5012, yadQ
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37019 (1-End)
      • engineered mutation (132)
    Domains in SCOPe 2.04: d4kk8a_
  • Chain 'B':
    Compound: H(+)/Cl(-) exchange transporter clcA
    Species: Escherichia coli [TaxId:83333]
    Gene: b0155, clcA, eriC, JW5012, yadQ
    Database cross-references and differences (RAF-indexed):
    • Uniprot P37019 (1-End)
      • engineered mutation (132)
    Domains in SCOPe 2.04: d4kk8b_
  • Chain 'C':
    Compound: Fab, heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KK8 (Start-221)
    Domains in SCOPe 2.04: d4kk8c1, d4kk8c2
  • Chain 'D':
    Compound: Fab, light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KK8 (0-210)
    Domains in SCOPe 2.04: d4kk8d1, d4kk8d2
  • Chain 'E':
    Compound: Fab, heavy chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KK8 (Start-221)
    Domains in SCOPe 2.04: d4kk8e1, d4kk8e2
  • Chain 'F':
    Compound: Fab, light chain
    Species: Mus musculus [TaxId:10090]
    Database cross-references and differences (RAF-indexed):
    • PDB 4KK8 (0-210)
    Domains in SCOPe 2.04: d4kk8f1, d4kk8f2
  • Heterogens: F

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4kk8A (A:)
mrrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadny
pllltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffgg
lgtlgggmvlgrqgptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnapla
gilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwly
lilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsgg
gfnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmva
velfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatll
aqftggkplysailartlakqeaeqk

    Sequence, based on observed residues (ATOM records): (download) 
    >4kk8A (A:)
rrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadnyp
llltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffggl
gtlgggmvlgrqgptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnaplag
ilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwlyl
ilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsggg
fnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmvav
elfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatlla
qftggkplysailartlakqea

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4kk8B (B:)
mrrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadny
pllltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffgg
lgtlgggmvlgrqgptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnapla
gilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwly
lilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsgg
gfnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmva
velfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatll
aqftggkplysailartlakqeaeqk

    Sequence, based on observed residues (ATOM records): (download) 
    >4kk8B (B:)
rrrqlirqllerdktplailfmaavvgtlvglaavafdkgvawlqnqrmgalvhtadnyp
llltvaflcsavlamfgyflvrkyapeaggsgipeiegaledqrpvrwwrvlpvkffggl
gtlgggmvlgrqgptvqiggnigrmvldifrlkgdearhtllatgaaaglaaafnaplag
ilfiieemrpqfrytlisikavfigvimstimyrifnhevalidvgklsdaplntlwlyl
ilgiifgifgpifnkwvlgmqdllhrvhggnitkwvlmggaigglcgllgfvapatsggg
fnlipiatagnfsmgmlvfifvarvittllcfssgapggifapmlalgtvlgtafgmvav
elfpqyhleagtfaiagmgallaasirapltgiilvlemtdnyqlilpmiitglgatlla
qftggkplysailartlakqeae

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4kk8C (C:)
evrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstiny
tpslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvs
sakttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqa
alytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

    Sequence, based on observed residues (ATOM records): (download) 
    >4kk8C (C:)
vrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstinyt
pslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvss
akttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqaa
lytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

  • Chain 'D':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4kk8D (D:)
divltqspaimsaapgdkvtmtcsasssvsyihwyqqksgtspkrwiydtskltsgvpvr
fsgsgsgtsysltintmeaedaatyycqqwsshpqtfgggtkleilradaaptvsifpps
seqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstltl
tkdeyerhnsytceathktstspivksfnra

  • Chain 'E':
    Sequence, based on SEQRES records: (download) 
    >4kk8E (E:)
evrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstiny
tpslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvs
sakttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqa
alytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

    Sequence, based on observed residues (ATOM records): (download) 
    >4kk8E (E:)
vrllesggglvqpggslklscaasgfdysrywmswvrqapgkglkwigeinpvsstinyt
pslkdkfiisrdnakdtlylqiskvrsedtalyycarlyygygywyfdvwgagttvtvss
akttppsvyplapgsaaaaasmvtlgclvkgyfpepvtvtwnsgslaagvhtfpavlqaa
lytlsssvtvpssswpsetvtcnvahpasstkvdkkivpra

  • Chain 'F':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4kk8F (F:)
divltqspaimsaapgdkvtmtcsasssvsyihwyqqksgtspkrwiydtskltsgvpvr
fsgsgsgtsysltintmeaedaatyycqqwsshpqtfgggtkleilradaaptvsifpps
seqltsggasvvcflnnfypkdinvkwkidgserqngvlnswtdqdskdstysmsstltl
tkdeyerhnsytceathktstspivksfnra