PDB entry 4kat

View 4kat on RCSB PDB site
Description: Crystal structure of FDTS from T. maritima mutant (R174K) with FAD and dUMP
Class: transferase
Keywords: thyX, FDTS, FAD, dUMP, Novel FDTS fold, Convertion of dUMP to dTMP using Tetrahydrofolate, TRANSFERASE
Deposited on 2013-04-22, released 2014-03-26
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-03-26, with a file datestamp of 2014-03-21.
Experiment type: XRAY
Resolution: 2.14 Å
R-factor: 0.185
AEROSPACI score: 0.34 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • expression tag (10-11)
      • engineered mutation (185)
    Domains in SCOPe 2.08: d4kata1, d4kata2
  • Chain 'B':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • expression tag (11)
      • engineered mutation (185)
    Domains in SCOPe 2.08: d4katb1, d4katb2
  • Chain 'C':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • engineered mutation (185)
    Domains in SCOPe 2.08: d4katc_
  • Chain 'D':
    Compound: Thymidylate synthase
    Species: Thermotoga maritima MSB8 [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • expression tag (11)
      • engineered mutation (185)
    Domains in SCOPe 2.08: d4katd1, d4katd2
  • Heterogens: DU, FDA, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4katA (A:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlkadshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4katA (A:)
hhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieylmkhghetpfe
hivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttipperv
tekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlkadsh
aqweiqqyalaiarifkekcpwtfeaflkyaykgdilk

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4katB (B:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlkadshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4katB (B:)
hmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieylmkhghetpfeh
ivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervt
ekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlkadsha
qweiqqyalaiarifkekcpwtfeaflkyaykgdilkevq

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4katC (C:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlkadshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4katC (C:)
mkidildkgfvelvdvmgndlsavraarvskdeerdrhlieylmkhghetpfehivftfh
vkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervtekisei
vdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlkadshaqweiqq
yalaiarifkekcpwtfeaflkyaykgdilkev

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4katD (D:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhghetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlkadshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4katD (D:)
hmkidildkgfvelvdvmgndlsavraarvsfeerdrhlieylmkhghetpfehivftfh
vkapifvarqwfrhriasynelsgryyefyipsperlegykttippervtekiseivdka
yrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlkadshaqweiqqyala
iarifkekcpwtfeaflkyaykgdilkev