PDB entry 4kas

View 4kas on RCSB PDB site
Description: Crystal structure of FDTS from T. maritima mutant (H53D) with FAD and dUMP
Class: transferase
Keywords: thyX, FDTS, FAD, dUMP, Novel FDTS fold, Convertion of dUMP to dTMP using Tetrahydrofolate, and NAD(P)Hc, TRANSFERASE
Deposited on 2013-04-22, released 2014-03-26
The last revision prior to the SCOPe 2.08 freeze date was dated 2014-03-26, with a file datestamp of 2014-03-21.
Experiment type: XRAY
Resolution: 1.85 Å
R-factor: 0.172
AEROSPACI score: 0.43 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • expression tag (10-11)
      • engineered mutation (64)
    Domains in SCOPe 2.08: d4kasa1, d4kasa2
  • Chain 'B':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-231)
      • expression tag (11)
      • engineered mutation (64)
    Domains in SCOPe 2.08: d4kasb1, d4kasb2
  • Chain 'C':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-231)
      • expression tag (11)
      • engineered mutation (64)
    Domains in SCOPe 2.08: d4kasc1, d4kasc2
  • Chain 'D':
    Compound: Thymidylate synthase thyX
    Species: Thermotoga maritima [TaxId:243274]
    Gene: thy1, thyX, tm0449, TM_0449
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT0 (12-End)
      • engineered mutation (64)
    Domains in SCOPe 2.08: d4kasd_
  • Heterogens: DU, SO4, FAD, CL, PO4, 2PE, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4kasA (A:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4kasA (A:)
hhmkidildkgfvelvdvmgndlsavraarvsfddeerdrhlieylmkhgdetpfehivf
tfhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervteki
seivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqwe
iqqyalaiarifkekcpwtfeaflkyaykgdil

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4kasB (B:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4kasB (B:)
hmkidildkgfvelvdvmgndlsavraarvsfddeerdrhlieylmkhgdetpfehivft
fhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervtekis
eivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqwei
qqyalaiarifkekcpwtfeaflkyaykgdilkevqv

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4kasC (C:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4kasC (C:)
hmkidildkgfvelvdvmgndlsavraarvsfdkdeerdrhlieylmkhgdetpfehivf
tfhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervteki
seivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqwe
iqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4kasD (D:)
mgsdkihhhhhhmkidildkgfvelvdvmgndlsavraarvsfdmglkdeerdrhlieyl
mkhgdetpfehivftfhvkapifvarqwfrhriasynelsgrysklsyefyipsperleg
ykttippervtekiseivdkayrtyleliesgvprevarivlplnlytrffwtvnarslm
nflnlradshaqweiqqyalaiarifkekcpwtfeaflkyaykgdilkevqv

    Sequence, based on observed residues (ATOM records): (download) 
    >4kasD (D:)
mkidildkgfvelvdvmgndlsavraarvsfdkdeerdrhlieylmkhgdetpfehivft
fhvkapifvarqwfrhriasynelsgrysklsyefyipsperlegykttippervtekis
eivdkayrtyleliesgvprevarivlplnlytrffwtvnarslmnflnlradshaqwei
qqyalaiarifkekcpwtfeaflkyaykgdilke