PDB entry 4k1u

View 4k1u on RCSB PDB site
Description: Crystal structure of delta5-3-ketosteroid isomerase containing Y16F and Y32F mutations
Class: isomerase
Keywords: Cystatin-like fold, allylic isomerization, delta5-3ketosteroids, cytosol, ISOMERASE
Deposited on 2013-04-05, released 2013-07-31
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-09-04, with a file datestamp of 2013-08-30.
Experiment type: XRAY
Resolution: 2 Å
R-factor: 0.225
AEROSPACI score: 0.41 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: steroid delta-isomerase
    Species: Pseudomonas putida [TaxId:303]
    Gene: ksi
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07445
      • engineered mutation (15)
      • engineered mutation (31)
    Domains in SCOPe 2.08: d4k1ua_
  • Chain 'B':
    Compound: steroid delta-isomerase
    Species: Pseudomonas putida [TaxId:303]
    Gene: ksi
    Database cross-references and differences (RAF-indexed):
    • Uniprot P07445
      • engineered mutation (15)
      • engineered mutation (31)
    Domains in SCOPe 2.08: d4k1ub_
  • Heterogens: HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4k1uA (A:)
mnlptaqevqglmarfielvdvgdieaivqmfaddatvedpfgqppihgreqiaafyrqg
lgggkvracltgpvrashngcgampfrvemvwngqpcaldvidvmrfdehgriqtmqayw
sevnlsvrepq

    Sequence, based on observed residues (ATOM records): (download) 
    >4k1uA (A:)
nlptaqevqglmarfielvdvgdieaivqmfaddatvedpfgqppihgreqiaafyrqgl
vracltgpvrashngcgampfrvemvwngqpcaldvidvmrfdehgriqtmqaywsevnl
sv

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4k1uB (B:)
mnlptaqevqglmarfielvdvgdieaivqmfaddatvedpfgqppihgreqiaafyrqg
lgggkvracltgpvrashngcgampfrvemvwngqpcaldvidvmrfdehgriqtmqayw
sevnlsvrepq

    Sequence, based on observed residues (ATOM records): (download) 
    >4k1uB (B:)
lptaqevqglmarfielvdvgdieaivqmfaddatvedpfgqppihgreqiaafyrqglv
racltgpvrashngcgampfrvemvwngqpcaldvidvmrfdehgriqtmqaywsevnls
v