PDB entry 4jav

View 4jav on RCSB PDB site
Description: Structural basis of a rationally rewired protein-protein interface (HK853wt and RR468mutant V13P, L14I, I17M and N21V)
Class: transferase/signaling protein
Keywords: Bergerat fold, Four helix bundle, alpha/beta fold, Signal transduction, Histidine kinase, autophosphorylation, phosphotransferase, dephosphorylation, TRANSFERASE-SIGNALING PROTEIN complex
Deposited on 2013-02-19, released 2013-09-04
The last revision prior to the SCOPe 2.07 freeze date was dated 2013-09-04, with a file datestamp of 2013-08-30.
Experiment type: XRAY
Resolution: 3.1 Å
R-factor: 0.204
AEROSPACI score: 0.24 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Histidine kinase
    Species: THERMOTOGA MARITIMA [TaxId:243274]
    Gene: TM_0853
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d4java1, d4java2
  • Chain 'B':
    Compound: Histidine kinase
    Species: THERMOTOGA MARITIMA [TaxId:243274]
    Gene: TM_0853
    Database cross-references and differences (RAF-indexed): Domains in SCOPe 2.07: d4javb1, d4javb2
  • Chain 'C':
    Compound: Response regulator
    Species: THERMOTOGA MARITIMA [TaxId:243274]
    Gene: TM_0468
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT9 (Start-121)
      • engineered mutation (12-13)
      • engineered mutation (16)
      • engineered mutation (20)
    Domains in SCOPe 2.07: d4javc_
  • Chain 'D':
    Compound: Response regulator
    Species: THERMOTOGA MARITIMA [TaxId:243274]
    Gene: TM_0468
    Database cross-references and differences (RAF-indexed):
    • Uniprot Q9WYT9
      • engineered mutation (12-13)
      • engineered mutation (16)
      • engineered mutation (20)
    Domains in SCOPe 2.07: d4javd_
  • Heterogens: SO4, MG, ADP, CL, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4javA (A:)
venvteskelerlkridrmktefianishelrtpltaikayaetiynslgeldlstlkef
leviidqsnhlenllnelldfsrlerkslqinrekvdlcdlvesavnaikefasshnvnv
lfesnvpcpveayidptrirqvllnllnngvkyskkdapdkyvkvildekdggvliived
ngigipdhakdrifeqfyrvdssltyevpgtglglaitkeivelhggriwvesevgkgsr
ffvwipkdragednrqdn

    Sequence, based on observed residues (ATOM records): (download) 
    >4javA (A:)
nvteskelerlkridrmktefianishelrtpltaikayaetiynslgeldlstlkefle
viidqsnhlenllnelldfsrlerkslqinrekvdlcdlvesavnaikefasshnvnvlf
esnvpcpveayidptrirqvllnllnngvkyskkdapdkyvkvildekdggvliivedng
igipdhakdrifeqfyrvdssltyevpgtglglaitkeivelhggriwvesevgkgsrff
vwipkd

  • Chain 'B':
    Sequence, based on SEQRES records: (download) 
    >4javB (B:)
venvteskelerlkridrmktefianishelrtpltaikayaetiynslgeldlstlkef
leviidqsnhlenllnelldfsrlerkslqinrekvdlcdlvesavnaikefasshnvnv
lfesnvpcpveayidptrirqvllnllnngvkyskkdapdkyvkvildekdggvliived
ngigipdhakdrifeqfyrvdssltyevpgtglglaitkeivelhggriwvesevgkgsr
ffvwipkdragednrqdn

    Sequence, based on observed residues (ATOM records): (download) 
    >4javB (B:)
teskelerlkridrmktefianishelrtpltaikayaetiynslgeldlstlkeflevi
idqsnhlenllnelldfsrlerkslqinrekvdlcdlvesavnaikefasshnvnvlfes
nvpcpveayidptrirqvllnllnngvkyskkdapdkyvkvildekdggvliivedngig
ipdhakdrifeqfyrvdssltyevpgtglglaitkeivelhggriwvesevgkgsrffvw
ipkd

  • Chain 'C':
    Sequence, based on SEQRES records: (download) 
    >4javC (C:)
mskkvllvddsapirkmvsfvlkkegyevieaengqialeklseftpdlivldimmpvmd
gftvlkklqekeewkripvivltakggeedeslalslgarkvmrkpfspsqfieevkhll
ne

    Sequence, based on observed residues (ATOM records): (download) 
    >4javC (C:)
skkvllvddsapirkmvsfvlkkegyevieaengqialeklseftpdlivldimmpvmdg
ftvlkklqekeewkripvivltakggeedeslalslgarkvmrkpfspsqfieevkhlln
e

  • Chain 'D':
    Sequence, based on SEQRES records: (download) 
    >4javD (D:)
mskkvllvddsapirkmvsfvlkkegyevieaengqialeklseftpdlivldimmpvmd
gftvlkklqekeewkripvivltakggeedeslalslgarkvmrkpfspsqfieevkhll
ne

    Sequence, based on observed residues (ATOM records): (download) 
    >4javD (D:)
skkvllvddsapirkmvsfvlkkegyevieaengqialeklseftpdlivldimmpvmdg
ftvlkklqekeewkripvivltakggeedeslalslgarkvmrkpfspsqfieevkhlln