PDB entry 4jad

View 4jad on RCSB PDB site
Description: STRUCTURAL DETERMINATION OF THE A50T:S279G:S280K:V281K:K282E:H283N VARIANT OF CITRATE SYNTHASE from E. COLI
Class: transferase
Keywords: citrate synthase, gram-negative bacteria, allostery, oxaloacetate, acetylcoa, nadh, allosteric enzyme, transferase, tricarboxylic acid cycle
Deposited on 2013-02-18, released 2013-07-17
The last revision prior to the SCOPe 2.08 freeze date was dated 2013-10-23, with a file datestamp of 2013-10-18.
Experiment type: XRAY
Resolution: 1.9 Å
R-factor: 0.194
AEROSPACI score: 0.38 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: citrate synthase
    Species: Escherichia coli [TaxId:83333]
    Gene: gltA, gluT, icdB, b0720, JW0710
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0ABH7
      • see remark 999 (9)
      • engineered mutation (49)
      • engineered mutation (278-282)
    Domains in SCOPe 2.08: d4jada_
  • Chain 'B':
    Compound: citrate synthase
    Species: Escherichia coli [TaxId:83333]
    Gene: gltA, gluT, icdB, b0720, JW0710
    Database cross-references and differences (RAF-indexed):
    • Uniprot P0ABH7 (0-425)
      • see remark 999 (9)
      • engineered mutation (49)
      • engineered mutation (278-282)
    Domains in SCOPe 2.08: d4jadb_
  • Heterogens: SO4, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4jadA (A:)
adtkakltldgdtaveldvlkgtlgqdvidirtlgskgvftfdpgftsttsceskitfid
gdegillhrgfpidqlatdsnylevcyillngekptqeqydefkttvtrhtmiheqitrl
fhafrrdshpmavmcgitgalaafyhdsldvnnprhreiaafrllskmptmaamcykysi
gqpfvyprndlsyagnflnmmfstpcepyevnpileramdrililhadheqnaststvrt
agssganpfaciaagiaslwgpahgganeaalkmleeigkkenipefvrrakdkndsfrl
mgfghrvyknydpratvmretchevlkelgtkddllevamelenialndpyfiekklypn
vdfysgiilkamgipssmftvifamartvgwiahwsemhsdgmkiarprqlytgyekrdf
ksdikr

  • Chain 'B':
    Sequence; same for both SEQRES and ATOM records: (download) 
    >4jadB (B:)
adtkakltldgdtaveldvlkgtlgqdvidirtlgskgvftfdpgftsttsceskitfid
gdegillhrgfpidqlatdsnylevcyillngekptqeqydefkttvtrhtmiheqitrl
fhafrrdshpmavmcgitgalaafyhdsldvnnprhreiaafrllskmptmaamcykysi
gqpfvyprndlsyagnflnmmfstpcepyevnpileramdrililhadheqnaststvrt
agssganpfaciaagiaslwgpahgganeaalkmleeigkkenipefvrrakdkndsfrl
mgfghrvyknydpratvmretchevlkelgtkddllevamelenialndpyfiekklypn
vdfysgiilkamgipssmftvifamartvgwiahwsemhsdgmkiarprqlytgyekrdf
ksdikr