PDB entry 4j8a

View 4j8a on RCSB PDB site
Description: Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145
Class: fluorescent protein
Keywords: beta-barrel, fluorescent protein, chromophore by cyclisation, p-azido-L-phenylalanine, cytosol
Deposited on 2013-02-14, released 2013-05-15
The last revision prior to the SCOPe 2.05 freeze date was dated 2013-06-26, with a file datestamp of 2013-06-21.
Experiment type: XRAY
Resolution: 1.26 Å
R-factor: 0.131
AEROSPACI score: 0.81 (click here for full SPACI score report)

Chains and heterogens:

  • Chain 'A':
    Compound: Green fluorescent protein
    Species: Aequorea victoria [TaxId:6100]
    Gene: GFP
    Database cross-references and differences (RAF-indexed):
    • Uniprot P42212 (2-End)
      • expression tag (0-1)
      • engineered mutation (30)
      • engineered mutation (39)
      • see remark 999 (66)
      • engineered mutation (80)
      • engineered mutation (99)
      • engineered mutation (105)
      • engineered mutation (145)
      • engineered mutation (153)
      • engineered mutation (163)
      • engineered mutation (171)
      • engineered mutation (206)
    Domains in SCOPe 2.05: d4j8aa_
  • Heterogens: SO4, EDO, TRS, HOH

PDB Chain Sequences:

  • Chain 'A':
    Sequence, based on SEQRES records: (download) 
    >4j8aA (A:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtl
vnrielkgidfkedgnilghkleynxnshnvyitadkqkngikanfkirhnvedgsvqla
dhyqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagithgmdelykg
shhhhhh

    Sequence, based on observed residues (ATOM records): (download) 
    >4j8aA (A:)
mvskgeelftgvvpilveldgdvnghkfsvrgegegdatngkltlkficttgklpvpwpt
lvttlgygvqcfsrypdhmkrhdffksampegyvqertisfkddgtyktraevkfegdtl
vnrielkgidfkedgnilghkleynxnshnvyitadkqkngikanfkirhnvedgsvqla
dhyqqntpigdgpvllpdnhylstqsvlskdpnekrdhmvllefvtaagit